Posted by admin on Dec 31, 2016 in |
The pharmacognostic evaluation, anatomy and antimicrobial potentials of the leaf, stem and root of Moringa oleifera Lam were evaluated. Powdered extracts obtained from the various plant organs were used for the analytical standardization. The methanolic plant extracts were assayed for antimicrobial activities using the Agar well diffusion method. This was compared with standard antibiotics, Gentamicin and ketoconazole. The Minimum Inhibitory Concentrations (MIC) of the methanolic plant extracts against the sensitive microorganisms was determined via the turbidity method. The results of the analytical standardization of the powdered leaf, stem and root of Moringa oleifera were found to be within pharmacopoeial standards. The results of the anatomical studies showed normal tissue distribution with distinctive features and structures, which could be instrumental for delimiting other closely related species. The phytochemical analysis revealed varying concentrations of alkaloids, tannins, flavonoids, saponins, steroids, terpenoids, resins, proteins and carbohydrates. The plants extracts exhibited antibacterial activities against all test bacteria. The stem extract particularly was better effective against Salmonella typhi than Standard antibiotic Gentamicin, with an MIC...
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Posted by admin on Dec 31, 2016 in |
To determine the Antimicrobial activity of fruit juices on the bacterial flora of mouth. The study is done to analyze whether the fruit juices helps in reducing the oral bacterial count which is constantly increasing. Fruits and vegetables are the main sources for essential nutrients. They also have a series of bio active components which have multiple effect on human general health. One such effect is, its Antimicrobial activity. More than 700 species of bacteria, fungi and parasites are present in the oral cavity as commensals. Among the fruit juices which are frequently consumed are apple, pomegranate and guava exhibit have an inhibitory effect on the oral bacterial flora and prevent them from causing dental caries and other oral lesions. The study is done using natural crude extracts of the fruit juices. After extraction they were sterilized by autoclaving at 1210c for 30 minutes. This is to mimic the natural environment of the mouth when the fruit juices are consumed. The study helps in better understanding the antimicrobial effect...
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Posted by admin on Dec 31, 2016 in |
Although there are numerous studies on antimicrobial activities of mushrooms against many common pathogenic microorganisms, there is hardly any study against Brucella spp., responsible for brucellosis in animals and human beings throughout the globe. The present study was aimed to evaluate the in vitro antimicrobial activities of ethanol extracts of five edible mushrooms namely, Pleurotus ostreatus, Pleurotus florida, Pleurotus eous, Pleurotus sajor-caju and Calocybe indica against four reference strains of Brucella spp.: B. abortus S19, B. abortus S99, B. melitensis M16 and B. suis 1330. Methodologies adopted were disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts. Evaluation of the antioxidant capacity of the extracts of the mushrooms by DPPH free radical scavenging activity was also aimed in this study. The total phenol content of the mushrooms showed significant positive Pearson correlation with the antioxidant capacity of the extracts. The results obtained revealed the antimicrobial potency of all the screened mushrooms against Brucella spp. P. sajor-caju had highest phenolic content, which may be...
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Posted by admin on Dec 31, 2016 in |
Background: Vidang (Embelia ribes Burm. f.) is a very famous plant for its anthelmintic activity. In Ayurveda, it is a first choice of drug for its krimighna action or to kill foreign pathogens. Embeliaro busta auct. Nonroxb., the other species of vidang is adulterated in Embelia ribes Burm. f. Therefore shastrokta or original vidang and its adulterant species were selected for the comparative study. Methods: Both aqueous and alcoholic extracts of fruits of both species of Embelia at 10, 20, 30% concentrations were tested for antimicrobial activity by agar well diffusion method against a range of gram-positive and gram-negative bacteria. Zone of inhibition of extracts were determined, then activity index and IC50 value was calculated. Results: Both ethanol and Aqueous extracts of Embelia ribes Burm. f. and Embeliaro busta auct. Nonroxb. inhibited the growth of all the tested strains of bacteria. Aqueous extract Embelia ribes Burm. f. has more potent action against microorganism Staphylococcus aureus, Escherichia coli, Klebsiellaaerogenes, ZOI (16mm,14mm,19mm respectively), AI(0.64, 0.78, 0.70 resp.), IC50 (1.751, 1.166, 1.641...
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Posted by admin on Nov 30, 2016 in |
Synthesis of 6-Methyl-2, 4-dihydro-3H-pyrazol-3-one- 4- (4-substituted) Benzylpiperazine derivatives IVP a-e was carried out by bromination of Ethyl aceto acetate (I) with KBr. The reaction was carried out in the presence of Hydrochloric acid and tolune to produce Bromo-ethyl aceto acetate (II), it is further condensed with substituted Benzylpiperazines in presence of ethanol to obtain condensed compound (III). This upon cyclization with excess of hydrazine hydrate will produce title compounds. All the title compounds IVP a-e were screened for possible antibacterial activity against P. Vulgaris, S. Aureas, E. Coli, B. Subtillus and antifungal activity against Altenaria, Culvalaria, C. Albicans and A. Niger. Among the compounds synthesized IVPb and IVPc demonstrated good antibacterial activity, IVb, IVc, and IVe showed good antifungal activity. The activities of the synthesized compounds are compared with the standard and other test compounds. The structures of synthesized compounds were established by elemental analysis, IR, H NMR and Mass spectral...
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