Posted by admin on Nov 30, 2016 in |
The antimicrobial effects of I. balsamina seed hexane extract against Staphylococcus aureus, Klebsiella pneumonia, Proteus vulgaris, and Serratia marcescens were determined using the standard technique. As well as the phytochemical properties of the extract on the test isolates were also examined using the standard methods. The phytochemical components of the hexane extract of the I.balsamina seed include falvonoids, alkaloids, phenol, tannin, alkaloids, steroid, saponin, phenol, flavonoids, triterpenes glycosides and carbohydrate. All the test organisms were susceptible to 25mg/ml of the extract. The MIC and MBC of the hexane extract of the I.balsamina against S. aureus and E. coli was 50mg/ml, while that of K. pneumonia and S. marcescens were 75mg/ml and 100mg/ml of the extract respectively. The result of this study suggests that the hexane extracts of I. balsamina could be suitable for the treatment of diseases infections caused by S. aureus, K.pneumonia, P. vulgaris and S....
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Posted by admin on Oct 31, 2016 in |
The preliminary phytochemical evaluation and effect of Honey on Hematological parameters were investigated. Preliminary phytochemical screening was carried out to determine the presence of various bioactive constituents through different Tests. The Antimicrobial study of different Honey in combination with synergistics was carried out on selective microorganisms through well diffusion method. The effect of Honey on hemoglobin, WBC, Blood Sugar and weight loss was studied on swiss albino male mice under controlled conditions for 21 days. The Highest percentage of coumarins (+++), Resins (+++) were found in all the types of tested Honey among the different phytochemicals Screened. However the absence of carboxylic carbon and tannins were noticed during the study. The antimicrobial studies along with different synergistics shown significant results like Natural Omani Honey exerted 36mm zone of inhibition against S.aureus, while Turkish honey found to be more antimicrobial against P. aeruginosa (40mm). In the present study on Hematological and physiological parameters it was observed that increase in WBC % with greatest significance, among all the tested Honey with...
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Posted by admin on Oct 31, 2016 in |
Development of microbial resistance leads new drug discovery, modification of antibiotics, administration of two or more antibiotics, use of traditional medicinal plants and their combinations with antibiotics. In the present study, 72 medicinal plants extracts were screened to potentiate the antimicrobial with erythromycin and amoxyclave. Among all the medicinal plant, Colebrookea oppositifolia was selected to analyze the synergistic activity with erythromycin and amoxyclave. The class I synergism (increase in the zone of inhibition) was observed in petroleum ether extract of leaves and inflorescence of C. oppositifolia in combination with amoxyclave with the increased zone from 6±0.2 mm to 8±0.2 mm and 6±0.2 mm to 10±0.2 mm, respectively. The class I synergisms also exhibited in methanol leaf extract of C. oppositifolia in combination with erythromycin and amoxyclave against S. aureus. Interestingly, the methanolic leaf extract (alone) did not show any antibacterial effect against S. aureus, but in combination with erythromycin/amoxyclave enhanced the zone of inhibition from 3±0.1mm to 9±0.2 mm and 5±0.2mm to 9±0.2mm, respectively. Class II synergism (making drug...
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Posted by admin on Sep 30, 2016 in |
Biofunctionalization of nanoparticles is an advancement of current nanotechnology and biotechnology fields. Developing biocompatible progressive functional materials possess antimicrobial properties could be hopeful for environmentally benevolent applications. Biofunctionalization of nanomaterials is one such topic to produce the non-toxic and more efficient antimicrobial agents. The present study describes a synthesis method of bioactive curcuminaniline functionalized zinc oxide nanoparticles in the eco-friendly methods and comparative studies of those were undertaken against non-functionalized zinc oxide nanoparticles. The synthesis process was carried out through a simple green methodology. Synthesized nanoparticles were analyzed for antibacterial activity by using disc diffusion method against two gram-positive bacteria and two gram-negative bacteria and antifungal activity by using agar well diffusion method against four funguses. Synthesized materials were characterized by UV- Vis, FT-IR, SEM and TEM techniques. The synthesized zinc oxide nanoparticles were in the range of 46 nm and the biofunctonalized nanoparticles were about 100 nm. The antimicrobial activities of those were showed appreciable inhibition zones against S.typhi, B.subtilis, A.niger and T.simii...
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Posted by admin on Jul 31, 2016 in |
Aims and Objectives: To evaluate the use and the impact of interventions on the use of antimicrobial agents (AMAs) at Primary Health Centres (PHCs) and Urban Health Centres (UHCs) in Ahmedabad district. Materials and methods: This was an interventional and prospective study carried out at 5 PHCs and 5 UHCs of ahmedabad district. In pre-intervention phase, 30 adult patients from each centre who were prescribed AMAs were included. Educational interventions were done for the prescribers by giving them booklet and by PowerPoint presentation about the use of AMAs for common diseases observed in the pre-intervention phase. In post-intervention phase, similar to pre-intervention phase, 30 adult patients from each centre i.e. 5 PHCs and 5 UHCs who were prescribed AMA were included. The impact of intervention and appropriateness of AMA use was measured using Modified Kunin’s Criteria. Results: Amongst a total of 300 patients, i.e. 150 each from PHCs and UHCs, the most commonly AMAs used were metronidazole and doxycycline respectively in pre-intervention phase. According to modified Kunin’s criteria, appropriate...
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