Posted by admin on May 30, 2015 in |
Diaporthephaseolorum is endophytic fungi isolated from Jatitree (Tectonagrandis L.f). Endophytic microbes were microbes that spent part or all of their life live within plant tissue of a host plant. They produce secondary metabolites with potent anti microbial activity. Jatitree (Tectonagrandis L.f) was one of herbal plants that act as host plants for endophytic microbes that produce secondary metabolites with anti-microbial activity. The current study focussed on isolation of the endophytic microbes from branches of Jati tree and their secondary metabolites. Isolation of endophytes were performed in PDY (potato dextrose yeast) and further extracted using EtoAC. Four fractions (F I – F IV) was obtained from EtOAc extract through column chromatography (n-Hex: EtoAc, ratio 50:1). Further, agar diffusion method was performed to assess their anti-microbial activity. Results demonstrated that F IV has potent towards Staphylococcus aureus (11.10mm). Its GC-MS profile strongly indicated the presence of fatty acid and phenolic related compound. In conclusion, secondary metabolites of endohpytes isolated from Jati tree branches contained predominantly phenol and fatty acid related compound...
Read More
Posted by admin on May 30, 2015 in |
In the present study, the hydro-alcoholic and hexane extracts of the plant, Valeriana jatamansi (Family: Valerianaceae) were screened for antimicrobial activity against pathogenic and drug resistant strains. The non polar extracts did not showed any activity against any of the pathogenic strains. Amongst these extracts the hydro-alcoholic extracts of the plant, Valeriana jatamansi, showed potent antimicrobial activity against almost all the pathogens studied. These extracts also showed potent antibacterial activity against multidrug resistant Staphylococcus aureus and Pseudomonas aeruginosa in the ranges from 0.3 mg/ml to 0.7 mg/ml. The potent hydro-alcoholic extract was further subjected for isolation and characterization of potent antimicrobial compound via chromatographic and spectroscopic techniques. The pure compound isolated, Valerenic acid was obtained as colorless liquid with a penetrating aroma; slightly soluble in water, soluble in alcohol, and ether. It melts at -34 0C and boils at 186 0C. The compound showed Rf value 0.37 similar to that of the standard compound, Valerenic acid as detected by TLC. The retention time of the pure compound purified was...
Read More
Posted by admin on Apr 30, 2015 in |
Spices are the potential source of natural products and naturally derived compounds. These compounds show antioxidant, antimicrobial properties. The aim of this study is to evaluate the antimicrobial properties of Cinnamomum zeylanicum (Cinnamon). In this study, antimicrobial activities of 70% ethanolic extracts of Cinnamomum zeylanicum were evaluated against different bacterial strains (E.coli, Pseudomonas aeruginosa, Candida albicans) by agar well diffusion method & MIC determination by broth dilution method. The extract showed a broad spectrum of antimicrobial activities by inhibiting the growth of respective microorganism in Agar well diffusion assay. The present study supports the immense medicinal properties of Cinnamomum zeylanicum. It may be insight for future researches in area of new drug development of herbal...
Read More
Posted by admin on Mar 30, 2015 in |
The aim of the present study was to isolate, identify and to analyze the phylogenetic characteristics of the potent actinobacterial strain VL-RK_05 isolated from Mango orchards of Vissannapet, Krishna Dist, Andhra Pradesh. Soil samples collected were pre treated with calcium carbonate and used for isolation of potent Actinobacterial strain designated as VL-RK_05. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S r RNA sequence based analysis. Phylogenetic tree was constructed using the Molecular Evolutionary Genetic analysis version 5. Phylogenetic analysis of 16S r RNA sequencing revealed that the strain is closely related to Rhodococcus erythropolis and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Shigella flexneri, Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida...
Read More
Posted by admin on Mar 30, 2015 in |
Ailanthus excelsa leaves were evaluated for antimicrobial activity against clinical isolates obtained from patients with eye and skin infections and also for its antioxidant potential. The bacterial isolates obtained were identified to be S. aureus, P. aeruginosa, E. coli and the fungal isolates were identified as Aspergillus niger and Penicillium. The extract showed a maximum zone of inhibition against A. niger followed by P. aeruginosa. But it did not exhibit any inhibitory activity against S. aureus. There was a remarkable inhibitory activity recorded against E. coli and Penicillium. The extracts were found to possess 0.7954 µg/g of total Tannins and 1.41µg/g of total Phenols. The extract also exhibited 12.5% radical scavenging activity in FRAP assay and 8.5% activity in CUPRAC...
Read More
