Posted by admin on Jan 31, 2015 in |
The purpose of this study was to evaluate antimicrobial activity of Bursera penicillata leaf extract against human infected bacteria. Antimicrobial activity of Petrolium ether, Methanolic and Aqueous extracts from leaves of Bursera penicillata were tested against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Staphylococcus saprophyticus, Enterococcus faecalis and Enterobacter cloacae using Agar well diffusion method. Solvent extracts from the leaves of the plant shown a considerable antimicrobial activity against most tested microorganisms. The most active extract was Methanolic extract from the leaf against Klebsiella pneumoniae and significantly inhibited the bacterial activity. Minimum inhibitory concentration for aqueous and methanolic extracts of leaves ranged from 5.0–20.0 mg/ml and ethyl acetate and petroleum ether leaf extracts ranged from 25.0µg-55.0 µg and 45.0-85 µg/ml. It further reflects a hope for the development of many more novel chemotherapeutic agents or templates from such plants which in future may serve for the production of synthetically improved therapeutic...
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Posted by admin on Jan 31, 2015 in |
Actinomycetes are versatile and prolific source of microbial natural products with great advantage in medicine and agriculture. Recently there is growing interest in exploring biological active compounds from rare actinomycetes. Usually rare actinomycetes are regarded as strains of actinomycetes whose isolation frequency by conventional methods is much lower than that of Streptomycetes strains. Amycolatopsis orientalis JAR10 rare genera of actinomycetes which have been isolated, characterized and antimicrobial activity against various pathogens have been determined in the present work. The morphological, cultural, genotypical and physiological characteristics of strain JAR10 has been examined by International Streptomyces Project. The bioactive metabolites produced by strain JAR10 were extracted by solvent extraction method and the crude extract obtained was further analyzed against various pathogens. The minimum inhibitory concentration of crude extract obtained from Amycolatopsis orientalis JAR10 was analyzed by broth dilution method and was found to inhibit Staphylococcus aureus at 60 µg/ml indicating the presence of biological active...
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Posted by admin on Jan 31, 2015 in |
This study was carried out with the objective of the antibacterial and antifungal activity of chloroform, ethyl acetate, ethanol, methanol and aqueous extracts of Asafoetida. Antibacterial activity was carried out against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and the antifungal activity was evaluated against Aspergillus niger, Candida albicans. The testing was done by well diffusion method and evaluation was done by detecting zone of inhibition (in mm) and minimum inhibitory concentration (MIC). Studies were perfomed with two different concentrations of all extracts (2 mg/ml and 4 mg/ml). Zone of inhibition were compared with standards like Ciprofloxacin (0.1 mg/ml) and Fluconazole (0.1 mg/ml). The results showed that ethyl acetate, ethanol, and methanol extract has significant antimicrobial activity and highest activity was reported with methanolic extract. The minimum inhibitory concentration of methanolic extract, ethanolic extract and ethyl acetate extract against most of the test microorganisms were 1 mg/ml, 1 mg/ml, 2 mg/ml...
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Posted by admin on Jan 31, 2015 in |
The compound 3-chloro-2-hydrazine quinoxaline was condensed with 2-furaldehyde to derive a Schiff base 2-furaldehyde-2-(3-chloro-2-quinoxalinyl) hydrazone (FCCQH).The ligand and also its complexes with VO (IV), Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Zn (II), and Pd (II) have been synthesized and characterized by elemental analysis, magnetic studies, thermal analysis, infrared spectra, UV-visible spectroscopy and Electron Spin Resonance data. The ligand 2-furaldehyde-2-(3-chloro-2-quinoxalinyl) hydrazone [FCCQH] acts as a neutral, bidentate one towards VO(IV), Zn(II), and Pd(II) coordinating through free C=N nitrogen and oxygen of furan ring and as tridentate one towards Cr(III), Mn(II), Fe(III), Co(II), and Ni(II) complexes coordinating additionally through nitrogen of quinoxaline ring C=N. The complexes have been screened for antibacterial activity towards staphylococcus aureus (gram +ve), Escherichia coli (gram -ve) bacteria, antifungal activity towards fusarium oxysporum and anticancer activities of the compounds were evaluated by MTT assay on HeLa (cervical cancer) cell lines. While the antimicrobial activity profiles of the complexes are, by and large quite encouraging, the anticancer activity exhibited by Ni (II) and Pd...
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Posted by admin on Jan 30, 2015 in |
Objective: Antibiotics resistance patterns for many bacteria have changed significantly; causing poor clinical outcomes. The objective of the study was to measure the susceptibility of different antibiotics against specific clinical isolates for surveillance purpose. Method: Kirby-Bauer disc diffusion method was used to evaluate the susceptibility pattern of four antibiotics (commercially available disc) i.e., Amoxicillin, Cefixime, Ciprofloxacin and Levofloxacin against different clinical isolates collected from various hospitals. The clinical isolates selected for the proposed study were of Staphylococcus aureus, Streptococcus Pneumoniae, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli. This collection of antibiotics and isolates were due to common pathogens and highly prescribed Oral antibiotics. Result: Results show that amoxicillin and cefixime are highly resistant; cefixime is sensitive against Salmonella typhi and Streptococcus Pneumoniae but 50 % resistant to the microbes. In quinolones, ciprofloxacin and levofloxacin have good sensitivity; Levofloxacin is a better choice against aforementioned pathogens. Conclusion: It was concluded that bacterial resistance rates vary among species and also from hospital to hospital. Irrational uses of antibiotics cause...
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