Posted by admin on Feb 28, 2017 in |
Lithiasis is the process of stone formation in the kidney, bladder, and/or urethra. Herbal drugs are reported to be effective in the treatment of urolithiasis with no side effects. Grewia asiatica is a plant commonly used as a traditional herbal medicine and possesses the wide range of pharmacological applications. The present study investigated the antilithiatic activity of an ethanolic leaf extract of Grewia asiatica Linn (EEGA). Ethylene glycol (0.75% in water) feeding resulted in hyperoxaluria as well as increased urinary volume and its pH. EEGA (200 & 400 mg/kg) was given orally in curative and preventive regimens over a period of 28 days. Supplementation with EEGA significantly (p<0.05) restored urea, uric acid, creatinine, volume of urine and pH levels. The preventive regimen was found to be better than the curative regimen. The results were comparable with the standard drug, Cystone (750 mg/kg). Histopathological examination further corroborated the antilithiatic or nephoprotective activity of the extract. The presence of triterpenoids, flavonoids and saponins in extract might be responsible for significant antilithiatic...
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Posted by admin on Jan 31, 2017 in |
Gas Chromatography-Mass Spectrometry (GC-MS) studies play an important role in isolation and identification of phytocompounds. Since, the chemical composition of the plants usually determines its biological activity, phytochemicals of possible pharmaceutical importance can be subjected to a detailed analysis. The present study is concerned with the GC-MS analysis of chloroform extract of the bark of Terminalia travancorensis, an evergreen tree, endemic to the Western Ghats. Reports on the benefits and or medicinal properties of T. travancorensis are rare or even absent. In this context, the present study was carried out to determine the chemical constituents of the bark by GC-MS analysis. Twenty two components were identified by GC-MS analysis in the chloroform extract. The major components detected were Friedelan-3-one (15.93%), gamma-sitosterol (10.48%), alpha -octadecene (8.54%), n-Tetracosanol-1 (8.21%), Phenol, 2,4-bis(1,1-dimethylethyl) (7.77%), 1-Nonadecene (7.49%), Heptacosanol (6.16%), 1-Heptadecene (5.25%) and stigmast-4-en-3-one (5.46 %). Friedelan-3-one, the major compound is a triterpenoid possessing antifeedant, anti-inflammatory, anticancer, hepatoprotective, antimicrobial and anticandidal activities. The second major compound, gamma-sitosterol plays a vital role in the structure and...
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Posted by admin on Nov 30, 2016 in |
Objective: The purpose of this study was to investigate the prevalence of non-polio enteroviral infections by quantitative and qualitative PCR assays in neonates 3-30 days old with clinical diagnosis of sepsis. Methods: The patients included all the neonates aged 3-30 days admitted with diagnosis of neonatal sepsis. Blood specimens, throat swabs, and in 60 cases CSF specimens were tested for Enteroviruses (EVs), using a quantitative RT-PCR assay. Alternate RT-PCR assays separated polio and non-polio enteroviruses. Demographic data including age, sex, type of feeding and gestational age were obtained. Results: As PCR results revealed, of the 177 neonates with clinical diagnosis of sepsis, one patient (0.55%) was positive for non-polio EVs in all of throat, serum and CSF samples. In 3 patients (1.7%), the results were positive for non-polio EVs in both throat and serum, while in15 (8.45%) others only throat samples were positive. Sex, age and gestational age had no significant relationship with the prevalence of the infections statistically (P>0.05). The prevalence of non-polio EV infections ranged from 6.1%...
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Posted by admin on Oct 31, 2016 in |
The antidyslipidemic and antioxidant activities of Allium sativum (Garlic) and Aloe vera (Ghee- kuwar) extracts have been studied in two models of hyperlipidemia, triton and cholesterol rich high fat diet induced hyperlipidemia. A. sativum, A. vera and Gemfibrogil were macerated with 0.2% aqueous gum acacia and the suspension was fed orally to rats of group III, IV and V at a dose of 400mg, 1g/Kg, and 50 mg/Kg (b.w.p.o.) respectively with triton. Serum lipids were found to be lowered by Allium sativum and Aloe-vera in triton induced hyperlipidemia. On the other hand chronic feeding of these extracts to animals in cholesterol rich high fat diet induced hyperlipidemia for 30 days caused lowering in lipid and protein levels of β lipoproteins followed by an increase in α lipoproteins. The results of present study demonstrate that hypolipidemic activity of these extracts mediate through inhibition of hepatic lipid synthesis, increased faecal bile acid excretion and induced plasma LCAT activity in rats. Furthermore these extracts (100 and 200 µg/ml) inhibited the in vitro...
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Posted by admin on Sep 30, 2016 in |
The natural acid like lemon juice provides mild acidic state which used to catalyzed reaction of primary amine and aldehyde in green solvent like ethanol at room temperature reflux for 1.5 hour gives Schiff bases in good yield. Schiff bases have attracted considerable attention of us due to their significant biological activities 1 like anticancer, antitumor, anti inflammatory agents 4, insecticidal, antibacterial 5, antituberculosis, antimicrobial 2-3, antifungal 5, anti-HIV and metal corrosion inhibition activities of Schiff bases, besides wide range of pharmacological activities. Natural acid is non-polluting and does not employ any lethal materials, quantifying if as a green approach for the synthesis of Schiff bases. Schiff bases also have several types of biological actions such as analgesic and antimalerial 6. They are working as Ligands for complexes of metal ions. Organosilicon complexes with Schiff bases are found to be beneficial in various agricultural applications like fungicides, bactericides, herbicides, acaricides and antifeedants 7. The metal complex of Schiff bases are also of principal significance in analytical chemistry and chemotherapy...
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