Posted by admin on Nov 30, 2016 in |
Adenosine has been proposed as a key factor regulating the metabolic balance between energy supply and demand in skeletal muscle. Adenosine A2B receptors are expressed across multiple tissues including skeletal muscle. Previously, it has been identified the robust (in vitro) induction of the nuclear receptors (NR4A) subgroup following adenosine A2B receptors stimulation in rat skeletal muscle cells. This may be concomitant with changes in the expression of genes involved in the regulation of nutrient metabolism. Considering that skeletal muscle is a primary tissue responsible for glucose disposal, glucose oxidation and insulin resistance, this study investigated a potential functional role for adenosine A2B receptors in rat L6 skeletal muscle myotubes in terms of glycolysis (HKII and PFK), and insulin resistance/glucose oxidation (PDK4) using a transcriptional approach (QRT-PCR (Probe-based)). For the first time in rat skeletal muscle cells, the results of the present study have shown that stable adenosine analogue NECA alters the expression of a number of genes (HKII, and PFK). Moreover, this is the first study demonstrating that selective...
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Posted by admin on Nov 30, 2016 in |
Adenosine is a strong modulator of gene expression, which underpins some of its potential expected effects in skeletal muscle in terms of fatty acid metabolism. Accumulating evidence highlights a critical role for the adenosine system in the regulation of fatty acid metabolism and obesity. However, its molecular signaling role in fatty acid transport and oxidation in skeletal muscle is less clear. The aim of the present study was to examine whether acute and chronic treatment of skeletal muscle cells in vitro with stable adenosine analogue NECA, selective adenosine A2B receptors antagonist/inverse agonist PSB 603, and/or selective adenosine A2A receptors agonist CGS 21680 affects mRNA expression of key metabolic genes in skeletal muscle. Rat L6 skeletal muscle cells were treated with NECA, PSB 603 and CGS 21680 for determination the mRNA expression of genes involved in fatty acid transport and oxidation (CPT1B, ACCβ and LCAD) using QRT-PCR (Probe-based). The results of the present study have shown for the first time that NECA alters the expression of a number of genes...
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Posted by admin on Nov 30, 2016 in |
Blepharis molluginifolia (Acanthaceae), is a threatened medicinal herb. The methanolic flower extract of Blepharis molluginifolia was subjected to Silica Gel Column Chromatography for purification of bioactive compounds and structure elucidation of active compounds were studied. DPPH and antimicrobial activities of two isolated compounds were studied. From the analysed data, the compounds identified were 5,7-Dihydroxy-3-(4-methoxyphenyl)chromen-4-one (BiochaninA) C16H12O5 and 5,7-Dihydroxy-3-(4-hydroxyphenyl) chromen-4-one (Genistein) C15H10O5. These compounds are first time reported in this plant. Another fraction GF1 was subjected to GCMS analysis and obtained 12 compounds (aminoacid, gamma lactone, sugars and fatty acids).As GCMS profile contained majority of sugars antiglycation was done against arbutrin and found >70% inhibition. The present investigation revealed that the methanolic flower extract contained phytoestrogens like Biochanin A and Genistein, the rare aminoacid pyroglutamic acid, gamma lactones, sugars and fatty...
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Posted by admin on Jul 31, 2016 in |
The brachial plexus block(BPB) has gained importance as regional anaesthetic technique for upper limb surgery. The primary aim of our study was to evaluate the addition of intravenous dexmedetomidine before BPB on overall patient satisfaction and sucess with objective of comparing the onset and duration of block, haemodynamic stability and sedation score. A randomized double blind prospective study was carried out on seventy patients of ASA grade I to III of either sex aged between 18 – 65 years scheduled for upper limb orthopaedic surgeries under BPB Who were randomly allocated into two groups: Group D (Dexmedetomedine) received Inj dexmedetomidine 1 µg/kg in intravenous 100 ml saline and Group P (Placebo) received intravenous 100 ml saline started 10 min before block. Onset and duration of block, quality of block, Haemodynamic variables, time to first rescue analgesics in post-op period, Sedation score, VAS score and Incidence of side effects and complications were recorded. Statistical analysis was done using Graph pad software. Hemodynamic variables, sedation score and satisfaction scores were analyzed...
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Posted by admin on May 31, 2016 in |
In the present investigation phenolic contents and antioxidant potential of Barleria prionitis L. leaf and stem extracts were analyzed by in vitro antioxidant assays and RP-HPLC method. The results indicated that high amount of total phenolics were present in acetone extracts of leaf (20.22±0.57 mg of GAE/g) and stem (21.19±1.25 mg of GAE/g). Highest flavonoid content was observed in ethanolic (44.23±2.82 mg of RE/g) and acetone extracts (44.94±0.85 mg of RE/g) of leaf, while stem extracts were poor in flavonoid contents. RP-HPLC method for determination of specific phenolic compounds was reported for the first time in B. prionitis L. Out of the phenolic compounds tested, Gallic acid was most common and more abundantly present in both leaf and stem of B. prionitis L. Aqueous extract of leaf showed highest extraction capacity for Gallic acid (211.70 μg/g) and Catechol (112.57 μg/g), while Caffeic acid (37.74 μg/g) and p-Coumaric acid (48.66 μg/g) were highest in methanolic extract. The present study indicated strong influence of extraction solvents and plant parts on antioxidant...
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