Posted by admin on Feb 28, 2022 in |
Background: Pomalidomide belongs to BCS class IV drug with low solubility and undergoes first-pass metabolism leads to reduced bioavailability of 15%. Objective: The current study aimed to develop pomalidomide Super saturable-SNEDDS to enhance solubility. Methods: Preliminary solubility studies were performed to identify oil, surfactant, and co-surfactant ratios. Pseudo tertiary phase diagram constructed to select the areas of nanoemulsion and based on monophasic region. Nineteen ratios were selected for drug loading study having oil: Smix ratio in 3:1, from which 15 formulations of pomalidomide SNEDDS were prepared with 4mg drug loading and screened for visual observation and turbidity measurement studies. These SNEDDS are further characterized for robustness, content of drug, entrapment efficiency, and in-vitro dissolution analysis. The optimized SNEDDS formulation was selected for screening of precipitation inhibitor by adding 2% precipitation inhibitors, and concentration-time profiles were studied under non-sink conditions from which best PI was selected and then in-vitro dissolution studies conducted for the super saturable SNEDDS (S-SNEDDS). The optimized final formulation S-SNEDDS with the highest drug release was analyzed...
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Posted by admin on Dec 31, 2021 in |
To study the hepatoprotective activity of ethanolic leaves extracts of Abroma augusta against non-alcoholic fatty liver disease to validate ethnobotanical claim regarding the plant used in the above mentioned disease. Materials and Methods: The hepatoprotective potential of Abroma augusta leaves extract (AALE) (250 and 500 mg/kg/ body weight) was studied on Methionine and Choline deficient diet (MCD diet), High Fat Diet (HFD), Cholesterol and Cholate diet (CCD) and streptozotocin + HFD induced non-alcoholic fatty liver disease. In the last treatment, blood was collected from direct cardiac puncture and analyzed for various parameters like alanine aminotransferase (ALT), aspartate transaminase (AST), triglycerides (TG), total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), free fatty acid (FFA) and malondialdehyde (MDA). Results: The present study showed for the first time that AALE reduced level of ALT, AST, TG, TC LDL, FFA, MDA and possessed hepatoprotective activity as evidenced by its significant inhibition in the formation of hepatoxicity induced by diet and chemical agents with maximum curation (500mg/kg/b.w.) against non-alcoholic fatty liver disease. It...
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Posted by admin on Jun 30, 2021 in |
Postoperative atrial fibrillation (POAF) is a frequent rhythm disturbance after cardiac surgery and is associated with poor cardiovascular outcomes. Beta-blocker therapy is an effective strategy to prevent or decrease the high incidence of POAF and β1-adrenergic receptor (β1AR) polymorphisms that affect the cardiac response to β1AR antagonists. The study objective is to analyze the association between genetic variations in the β1AR gene and the risk of POAF and the prophylactic effect of beta-blockers. Totally, 198 patients undergoing first-time cardiac surgery were examined. The patients after operation were grouped as either with POAF or without POAF according to their continuously electrocardiogram monitoring within ICU and hospital admission period. Subsequently, genotype frequencies of the β1AR gene were determined by polymerase chain reaction-restriction fragment length polymorphism. The results showed that 63 patients (31.8%) of the total study population developed POAF. After the application of a multivariate logistic regression model, the incidence of POAF was associated with Arg389Gly genotype (OR=2.48; 95% CI, 1.06-5.76; p=0.036), Arg389Arg genotype (OR=3.38; 95% CI, 1.28-9.33; p=0.018) and Arg389Ser49...
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Posted by admin on Feb 28, 2021 in |
This study reports the isolation and antioxidant activity of non-polar terpenes extracted from the leaves of Bougainvillea glabra choicy. To characterize the phytochemical constituents of Bougainvillea glabra leaves using the GC-MS method. The 95% ethanol extract was filtered, distilled, and concentrated to obtain the solid greenish residue. The residue was further fractioned by n-hexane, chloroform, ethyl acetate, acetone, ethanol, and methanol. The GC-MS analysis of Chloroform extract of Bougainvillea glabra leaves revealed the presence of thirteen compounds that could contribute to the medicinal quality of the plant. The first compound identified with less retention time (9.50 min) was 1-(1-methyl-decyl)-decahydro-napthalene. The isolation of organic compounds was done using column and thin layer chromatographic techniques. Compound characterization using various spectroscopic techniques identified the final isolated compound as 1-(1-methyl-decyl)-decahydronapthalene. Antioxidant activity was evaluated by the DPPH method. The method of isolation is simple, cost-effective, and...
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Posted by admin on Jan 31, 2021 in |
In recent years, the extensive use of antibiotics has resulted in their frequent detection in the effluent discharge of wastewater discharge plants (WWTPs). In this study, the feasibility of using Surfactant-Modified Kaolinite (SMK) to remove the antibiotic, Ciprofloxacin (CIP) from wastewater was evaluated from batch experiments. The batch adsorption system was utilized to evaluate the CIP removal efficiency at different contact times, SMK dosages, and CIP concentrations. The experimental data were analyzed by the Langmuir, Freundlich, Temkin, and Dubinin–Radushkevich (D–R) isotherm models. R2 values of Langmuir and D–R isotherm models are higher than that of other models, and maximum monolayer coverage (qmax) was calculated as 61.82 mg/g. The separation factor of 0.495 indicated favorable sorption. Also, from the Freundlich isotherm model, the intensity of adsorption (n) that indicated favorable sorption was 2.954. The kinetic study of sorption indicates that the pseudo-second-order model provides a better correlation of the sorption data than the pseudo-first-order, confirming the chemisorption of CIP ions on SMK. The results showed that a significant increase in...
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