Posted by admin on Nov 30, 2019 in |
In this study we aimed at isolation, identification, and quantification of two phenolic compounds reported for the first time in leaves of Amaranthus hypochondriacus. A non targeted GC-MS metabolite profiling of hexane extracts of amaranth leaves was done which indicated the presence of major phenolic compounds. Two alkylated phenols namely 2,4-di-tert-butyl phenol (A) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) were isolated for the first time from the hexane extract of A. hypochondriacus leaves. Structure elucidation of both the compounds was done using 1H NMR and purity was checked by GC-MS. Quantitative estimation of purified compounds in leaves extract was carried out using RP-analytical HPLC. The antioxidant potential of the isolated compounds (A), (B) and the hexane extract of leaves were investigated using in-vitro 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radicals scavenging bioassay. Hexane leaf extract (IC50 = 385.34 ± 37.11 µg/mL), 2,4-di-tert-butyl phenol (A) (IC50 = 253.76 ± 24.67 µg ml-1) and 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propanoic acid (B) (IC50 = 174.09 ± 16.89 µg ml-1) exhibited significant free radicals scavenging activity. Thus, our study reports...
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Posted by admin on Oct 31, 2018 in |
Bromelain is a protease present in pineapple fruit and stem. It is reported that commercially available pure bromelain has anticancer, anti-inflammatory and other therapeutic effects. But there are few reports available on the biological activity of bromelain along with peroxidase which is present in pineapple fruit extract. The aim of the present study was to investigate the effect of bromelain with peroxidase from pineapple fruit against lymphoma cell line in in-vitro. Pineapple was purchased from the local market from Tripura. Bromelain isolated along with peroxidase and enzyme activity tested with the substrate. In-vitro cytotoxicity was determined by MTT assay, and apoptosis was determined by fluorescence microscopy, DNA fragmentation assay and by western blot analysis. Fluorescence microscopy analysis and the MTT assay showed significant apoptosis of lymphoma cell after treated with bromelain along with peroxidase in a dose- and time-dependent manner and was more apoptotic than bromelain alone. This was further confirmed by DNA fragmentation assay. Furthermore, western blotting analysis confirmed the mitochondrial pathway is involved in apoptosis of lymphoma...
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Posted by admin on Sep 30, 2018 in |
Naturally occurring non-starch polysaccharides are known for their therapeutic value. This paper describes isolation, characterization and evaluation of glucose lowering potential of one such plant polysaccharide isolated from the leaves of the plant Vitex negundo. It was isolated and characterized using various analytical techniques and biochemical tests which confirmed the presence of carbohydrates and elements such as C, H and O. It demonstrated a reduction in glucose concentration in-vitro, however non-significant in comparison to standards. In-vivo, it was given per orally in fasting male C57 mice at 10, 30 and 100 mg/kg single dose post 30 min of which serum glucose was recorded. Glucose load was given 30 min after administering crude polysaccharide after which serum glucose concentrations were estimated at 15, 30, 60 and 120 min. Obtained results suggest a non-significant reduction in AUC as well as individual glucose levels at all the time points in crude polysaccharide treated animals in comparison to vehicle control in fasting as well as glucose loaded conditions. Thus, the crude polysaccharide isolated...
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Posted by admin on Sep 30, 2018 in |
Polyalthia korinti (Dunal) Benth. & Hook. F. is a rare Indo – Sri Lankan species of Annonaceae family and are traditionally used by tribal people of Ghats region, India for treating several ailments. The number of mature plants and the area of its distribution are too low and this species is placed under the vulnerable critically endangered category. The present study deals with the pharmacognostic and physicochemical evaluations of the leafy part of this plant and was undertaken as an initial step to aid in documentation, quality assurance, proper identification etc. and appropriately to investigate the plant material for its ethanomedicinal claims. Macro and micro morphological parameters of leaf and dried leaves powder were set down following standard procedures recommended by Ayurvedic Pharmacopoeia of India. Macroscopic study of leaf revealed some of the characteristic features like size, shape and color and in microscopic study internal characteristic features like epidermis, cortex, xylem, phloem, trichomes, and stomata were noted. Physicochemical parameters like total ash value, water soluble ash, acid insoluble ash,...
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Posted by admin on Aug 31, 2018 in |
Intravenous administration of 5-Fluorouracil (5FU) produces severe systemic, dose-related side effects due to its cytotoxic nature, additionally, 5FU is rapidly absorbed from the stomach after oral administration. Therefore a combined floating-bioadhesive tablet (FBT) of 5FU has been developed in order to reduce the dose and associated side effects in other sites of the body. The tablets were prepared by direct compression technique. Hydroxy propyl methyl cellulose (HPMC) K15M was used as a release retardant polymer while carbopol-971P, guar gum and sodium alginate were used as bioadhesive polymer. The pre-compression characteristics shown fair flow behaviour and core tablet properties were within the Pharmacopoeia limit. All formulation batches reported satisfactory floating lag time in a range of 125 – 500 sec and remained buoyant for more than 6 h. Kinetics of dissolution data demonstrated that formulation batch FB4, FB9 and FB10 follow Korsmeyer-Peppas model, FB11 follows first-order kinetics; and FB1, FB2, FB3, FB5, FB6, FB7, FB8, FB12 and FB13 follows Higuchi Matrix model. Analysis of the data revealed that formulation batch...
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