Posted by admin on Jul 31, 2015 in |
The present study was done to evaluate in-vitro membrane stabilizing activity with human RBC, in-vivo neuropharmacological activity including open field and swimming test, gastrointestinal motility and acute toxicity of different leaf extracts of Spondias pinnata in Swiss albino mice following oral administration. In-vivo GI motility test was done by charcoal induced anti motility test, acute toxicity study was done by investigating mortality/morbidity status of test animal and in-vitro membrane stabilizing activity was done with human RBC in both hypotonic and heat induced method. In case of in-vitro membrane stabilizing activity for hypotonic solution induced haemolysis, the ethanol extract inhibited 76.92% haemolysis of RBCs as compared to 85.42% produced by acetyl salicylic acid and during heat induced condition different organic soluble materials of S. pinnata demonstrated 76.68%, 77.19% and 74.83% inhibition of haemolysis of RBCs respectively whereas ASA inhibited 78.04%. In-vivo neuropharmacological activity showed significant (p<0.05) and (p<0.001) results when methanol 200 mg/kg, ethanol 200 mg/kg, chloroform 100 & 200 mg/kg extracts were administered to evaluate the rate of movement...
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Posted by admin on Jul 31, 2015 in |
Background: Sepsis is a significant public health concern and is the main cause of death in surgical intensive care units (ICUs). Patients with sepsis have features consistent with immunosuppression. Taurine is rapidly emerging as one of the more interesting amines which has been reported to have immune-modulatory effect through its action on cytokines. Purpose: To determine the effect of using Immune-enhancing enteral nutritional feed containing taurine compared to Standard enteral nutritional feed on clinical outcomes of ICU septic population. Methods: This was a prospective, randomized, controlled study. A total of 45 patients were randomly divided into 3 groups: Group 1 (n=15) received Standard Enteral Nutrition feed for two weeks, Group 2(n=15) received Immune-Enhancing Enteral Nutrition feed containing 10 mg/kg/day of Taurine for two weeks and Group 3(n=15) received Immune-Enhancing Enteral Nutrition feed containing 30 mg/kg/day of Taurine for two weeks. Parameters measured were serum levels of Interleukin-6, Interleukin-10, C-reactive protein and Total leukocyte count. Results: The current study showed that Taurine had immune-modulatory effect by significantly decreasing the level...
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Posted by admin on Jul 31, 2015 in |
In this study, the possible influence of the model, cationic drug chlorpromazine hydrochloride (CPZ-HCl) concentration on the micro-electrophoretic mobility of neutral large multi-lamellar dimyrstoylphosphatidylcholine (DMPC) liposomes was investigated in various media at both 25oC and 37oC. In addition the effect CPZ-HCl concentration on the micro-electrophoretic mobility of both positively charged DMPC/stearyl amine (ST) liposomes and negatively charged DMPC/dicetyl phosphate (DCP) liposomes were also investigated. The effect of pH on the micro-electrophoretic mobility of neutral positively charged and negatively charged liposomes in the absence and presence of CPZ-HCl in water and in 0.1M KCl at 25oCwere also studied. Lastly, the influence of CPZ-HCl at concentrations known to cause anesthesia, on the micro-electrophoretic mobility of neutral, positively charged and negatively charged liposomes in phosphate buffer (pH 7.4) at 37oC was also investigated. Present study indicates that DMPC liposomes in water exhibited zero electrophoretic mobility over a wide pH range. DMPC liposomes acquired negative charges in 0.1M KCl, phosphate buffer (pH 6.0 and 7.4) and in human plasma (pH 7.4). The addition...
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Posted by admin on Jul 31, 2015 in |
The oil polluted soil has many hydrocarbons which can be degraded by microbes. These oil degrading microbes were isolated from the oil contaminated soil through plate and dilution technique. The oil contaminated soils were collected from the oil spilled places of Vaniyambadi and Ambur areas of Vellore District, Tamil nadu, India. Through the biochemical tests, the isolated bacterial strains were identified as Pseudomonas aeruginosa and Bacillus subtilis, the strains were further confirmed by starch hydrolysis test and by growing on cetrimide agar. It is screened for the biosurfactant production through drop collapsing test and Emulsification Index (E24) using diesel, petrol, kerosene and tween 20. Biosurfactants are amphiphilic compounds which reduce surface and interfacial tension. Here, Pseudomonas aeruginosa had shown higher biosurfactant activity, when compared to Bacillus...
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Posted by admin on Jul 31, 2015 in |
Four simple, rapid, inexpensive, precise and accurate UV spectrophotometric methods have been developed for simultaneous estimation of Aspirin (ASP) and Lansoprazole (LANSO). Method A was Simultaneous equation method (Vierodt’s method) which applies measurement of absorptivities at two wavelengths, 276.00 nm, (λmax of Aspirin) and 284.00 nm, (λmax of Lansoprazole) in zero order spectra. The concentrations were calculated from the derived equations. Method B was based on zero crossing first Derivative (D1) spectrophotometry where Aspirin showed zero crossing point at 303nm and Lansoprazole showed zero crossing point at 244.5nm.Method C was Dual wavelength technique, in which absorbance difference between two points on the mixture was measured where difference for one drug is zero and amplitude of other drug was directly proportional to the concentration. Analytical wavelengths for Aspirin were 262nm and 295.7nm; while for Lansoprazole 270nm and 282.5nm were selected. Method D was based on Multicomponent mode technique, in which sampling wavelengths selected were 276 and 284 nm. Linearity for Aspirin was between 26-130 µg/mL and Lansoprazole was 4-20 µg/mL. ...
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