Posted by admin on Oct 31, 2015 in |
Di-butyl phthalate (DBP) is an environmental contaminant used in the production of plastics, cosmetics and medical devices. In the present study, the effects of exposure to DBP on reproduction of female mice were investigated. Adult female mice given DBP through gastric intubation at 0 (control), 250 (low dose) and 1000 (high dose) mg / Kg BW/ day for 10 days. After treatment, body weight and ovarian weight of DBP treated mice decreased significantly at high dose while uterine weight decreased significantly both low and high dose as compared to control. Gonadosomatic index (GSI) and uterosomatic index (USI) of these mice were found significantly lower at both doses of DBP. DBP treated mice showed altered estrous cyclicity at both DBP doses as compared to control. Serum estradiol level was found significantly lower but the progesterone level was normal in DBP treated mice as compared to control. In ovary total cholesterol level was almost same in all three groups but activity of 3-β hydroxysteroid dehydrogenase (3-β HSD) was found significantly lower...
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Posted by admin on Oct 31, 2015 in |
This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of Acetylcysteine and Cefixime in pharmaceutical formulation in the presence of degradation products. The separation was achieved using a Kromasil C8 reverse phase column (25X 0.46mm I.D, 5µ particle size) at room temperature with an isocratic mixture of methanol:KH2PO4 (50mM, pH 3.0) (30:70) at a flow rate of 1ml/min and detection at 228 nm. To establish stability indicating capability of the method, drug product was subjected to the stress conditions of acid, base, oxidative, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved from Acetylcysteine and Cefixime. The developed method was validated as per ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of Acetylcysteine and Cefixime in pharmaceutical preparation. It can be readily utilized for Quality Assurance (Q.A) and Research and Development (R&D) laboratories of pharmaceutical...
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Posted by admin on Oct 31, 2015 in |
Cancer is a common and widespread cause of human death in the world. Treatments like chemotherapy and radiotherapy facilitate in prolonging the patient’s life however in some cases cancer can be prevented. Cancers like breast, prostate, colon, renal cell carcinoma are caused by the overexpression of an enzyme termed Carbonic Anhydrase IX (CAIX). In 2015, 60,290 new cases of breast cancer alone are estimated. If this overexpression is controlled using an inhibitor, then the mortality rates due to cancer can be brought down by a significant margin. The primary objective of this research is to find an inhibitor that would reduce or down regulate the overexpression of CAIX in the human body. The inhibitor would specifically target CAIX. CAIX has a region which is unique and its cavities were identified. These were docked with the chosen inhibitor. Numerous acetazolamides and sulfanilamides were tested as inhibitors. Various bioinformatics tools such as Maestro and Hex were used for docking and grid generation. Docking scores were obtained and studied. The inhibitors with...
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Posted by admin on Oct 31, 2015 in |
Objectives: The present study was conducted to evaluate and compare the effect of vanadium pentoxide and chromium oxide in normal and streptozotocin induced diabetic albino rats. Methods: Diabetes was experimentally induced by injecting intraperitoneally with a single dose of 60mg/kg. The animals were considered as diabetic, if their blood glucose values were above 300 mg/dl on the 10th day after injection. The blood glucose estimation was done by glucose oxidase method. Glibenclamide was taken as standard drug. The one-way ANOVA followed by Dunnett’s ‘t’ test was used for statistical analysis. Results: The blood glucose levels were found to be significantly (p<0.05) decreased in vanadium pentoxide and chromium oxide treated groups. Conclusion: This study suggests that vanadium pentoxide and chromium oxide have antidiabetic effect in a dose dependent...
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Posted by admin on Oct 31, 2015 in |
Sophisticated analytical methods like HPLC and Mass spectroscopic methods which being employed for analysis, are relatively expensive and hence there is a need for developing simple analytical methods. In the proposed work, such methods have been developed and applied for routine determination of ketorolac in pharmaceutical formulations and bulk dosage forms. These methods were based on the formation of colored species on binding of ferric chloride and Hydrochloric acid for method A and binding of drug and Folin Cioclataeu reagent in alkaline conditions for method B, and the colored chromogen obtained in each method was finally treated with the drug ketorolac to produce yellow colored for method A and blue colored chromogen for method B with λmax at 430 nm and 627 nm respectively. Statistical analysis of these methods exhibited Sandell’s Sensitivity of 0.8 and 0.0617 (Method A and B) respectively, and the relative standard deviation (RSD) of these methods were found equal to 0.69 and 1.90 respectively, indicating that these methods are highly reproducible, based on the principle...
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