Posted by admin on Aug 31, 2015 in |
The study was carried out to investigate the essential oil composition and antibacterial effects of Eugenia cotinifolia ssp. codyensis leaves. The fresh leaf essential oil was extracted hydrodistillation process using Clevanger apparatus. The compounds of the essential oil were analyzed using Gas Chromatography Mass Spectrometry (GC-MS) technique identified a total of 84 numbers of chemical constituents and resulted 99.99%. The essential oil was characterized by high content of sesquiterpene compounds (79.23%) and the major constituent being Germacrene D (17.95%). The leaf oil exhibited higher antibacterial effects against gram negative bacteria compare to gram positive bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the oil against the organism ranged between 0.25 – 0.75 mg/ml and 0.5 – 2.5 mg/ml respectively. The essential oil exhibited maximum inhibition at 0.25 mg/ml against Proteus vulgaris and the same inhibited Bacillus subtilis and Staphylococcus aureus at 0.75 mg/ml. The result of essential oil chemical constituents and antibacterial activity showed useful for preparation of commercial...
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Posted by admin on Aug 31, 2015 in |
The aim of the present study was to investigate the antifungal potential of trypsin inhibitors (AMTI-I and AMTI-II) isolated from the seeds of Abelmoschus moschatus on selected pathogenic fungal strains. The purified inhibitors have been found to be homogenous by the criteria of native PAGE and gel filtration with apparent molecular weights of 22.4kDa and 21.2 kDa as judged by SDS-PAGE. While both the inhibitors were strongly active against bovine trypsin, they showed moderate effect on porcine elastase. AMTI-I and AMTI-II significantly affected the growth of Candida albicans, Candida tropicalis, Asperigillus flavus, Saccharomyces cerevisiae, Candida glabrata and Asperigillus niger with notable zones of inhibition. The inhibitors, however, did not show any inhibitory effect on the growth of other fungal strains- Fusarium oxysporum, Alternaria alternate, Mucor indicus and Penicillium chrysogenum. The fungicides, Flucanazole and Ketoconazole were used as positive controls in this study. Results obtained suggest that AMTI-I and AMTI-II may be regarded as excellent candidates for the development of novel antimicrobial agents against human pathogenic...
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Posted by admin on Aug 31, 2015 in |
Cichorium intybus (Asteraceae) is used as traditional medicine in India for various liver related disorders. The present study evaluates the hepatoprotective potential of leaf extract on N- nitrosodiethylamine induced hepatotoxicity, which is commonly present in foods, beverages, tobacco smoke, herbicides, pesticides, drinking water, and industrial pollution. The leaves were sorted as per their size (short, medium, large) and subjected to extraction with ethanol, water and ethanol: water (1:1 w/w) by cold maceration and hot soxlation. The extract having the highest extractive value 80.7%w/w was selected for animal studies. Group I, II, III, served as control, toxic and standard. Group IV and V were post treatment receiving 400 mg/kg body weight and 800 mg/kg body weight respectively and group VI as pre treatment group receiving 800 mg/kg body weight of the extract before the induction of toxicity. The level of serum markers such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) were significantly suppressed (P<0.0001) in both the groups receiving 400 mg/kg body weight and 800 mg/kg body...
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Posted by admin on Aug 31, 2015 in |
Objective: To evaluate hypolipidemic potential of Kasni (Cichorium intybus) seed preparations combined with oral hypoglycemic agent in treatment of patients of newly diagnosed type 2 DM. Methods: Newly diagnosed 90 patients of Type2DM, age 35-65years, of either sex were divided into 3 groups. Each group having 30 patients (19 male and 11 females) matched with each other in terms of age and sex. In group I only Glycomet SR once a day and in group II/III 6 grams crude seed powder or 50 ml decoction of crude seed powder twice a day in combination with Glycomet SR once in a day, was given for 90 days. Serum cholesterol, triglyceride, LDL and HDL levels were measured at zero, 30th, 60th and 90th day. Results: All the three groups showed a significant reduction in cholesterol, triglyceride and LDL across the four time periods. Post hoc Tukey HSD test shown that there was a significant difference between group I & II (p=0.045) and group I & III (p=0.000) for cholesterol; group I...
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Posted by admin on Aug 31, 2015 in |
The solid microemulsion of Darunavir, a promising antiretroviral drug, is attempted in the present work for enhancing the solubility and dissolution rate. For this purpose, solubility of Darunavir was determined in various oils, surfactant and cosurfactants. Pseudo-ternary phase diagrams were constructed to identify the microemulsion existing zone. The optimized formulation consisted of 65% of Capmul MCM, 20% of Cremophore RH 40: Transcutol P (1:1) and distilled water. The optimized microemulsion formulation was characterized for its refractive index, % transmittance, pH, viscosity, drug content and particle size. Particle size of the optimized microemulsion formulation was found to be 40.68 nm. Various adsorbents were incorporated in the optimized liquid microemulsion to get solid microemulsion. The solid microemulsion with aerosil 200 was optimized because of very low concentration (1:1) of aerosil was incorporated in microemulsion as compare to other adsorbent. The prepared solid microemulsion was subjected to characterization for angle of repose, bulk density, tapped density, Hausner’s Ratio, Carr’s index, drug content, ex-vivo drug release study and stability studies. The solid microemulsion...
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