Posted by admin on May 30, 2015 in |
A simple, precise, specific and accurate high-performance thin-layer chromatographic method has been developed for the simultaneous determination of Emtricitabine (EMB) and Tenofovir (TEN) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica gel 60 F254, (20×10 cm) with 250 μm thickness using toluene: ethylacetate: methanol in the ratio (2:8:1 v/v/v) as mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 272 nm. The drugs were resolved satisfactorily with Rf values of 0.44 and 0.68 for EMB and TEN, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9999 and 0.9995 for EMB and TEN, respectively, in the concentration range of 40–400ng spot−1 for EMB and 200–700ng spot−1 for TEN. The method was validated for precision, robustness, specificity and accuracy. The limits of detection and quantitation were 20 and 40ng spot−1, respectively, for EMB and 100 and 200ng spot−1, respectively, for TEN. The proposed...
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Posted by admin on May 30, 2015 in |
In the present study, high performance thin layer chromatographic method was developed and quantification of Diosgenin from Albizia lebbeck. TLC auminium precoated with silica gel were used as the stationary phase in a twin see through glass chamber saturated with n-butanol: glacial acetic acid: water (4:1:1) mobile phase, performed at room temperature (25ºC ± 2). The Rf value of diosgenin was found to be 0.8. Linearity was found to be in the concentration range 2 to 10 µg/spot. The linearity regression analysis of calibration plots showed good linear relationship between peak area and peak height (r2= 0.996). The result showed that the chromatogram of Diosgenin was performed in 3D display of diosgenin and Overlay of diosgenin and ethanolic bark extract of Albizia...
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Posted by admin on May 30, 2015 in |
The aim of the present study is to identify the volatile, bioactive phytoconstituents present in the ethanolic extract of the leaves of Glochidion ellipticum Wt. (Euphorbiaceae) was carried out by using GC-MS analysis, while mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology and Wiley library. Twenty seven components from leaves of the above said plant were identified. The active principles with their retention time, molecular formula, molecular weight and concentration (%) in the ethanol extract of leaf of G. ellipticum are obtained. This is the first report of documentation of active constituents from leaves of Glochidion ellipticum. The research reveals the potential of G. ellipticum leaves as a good source of bioactive compounds such as esters, ethers, alkaloids, alkenes, fatty alcohols, terpenoids, amines, porphyrin and silicon compounds that justify the use of this plant for its various ailments by traditional...
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Posted by admin on May 30, 2015 in |
The ligands bis-(benzimidazolyl)pyridine (BBIP) and bis-(benzimidazolyl)benzene (BBIB) have been used to synthesize Copper(II) complexes [Cu(BBIP)2]Cl2 and [Cu(BBIB)2]Cl2 respectively. The complexes are characterized by analytical and spectral (FTIR, 1H NMR and electronic) techniques. IR spectra of complexes show that the tertiary nitrogens in the ligand are involved in the co-ordination to the metal ion. An octahedral geometry has been suggested for the complexes and the molar conductance values reveal them to be 1:2 electrolytes. The TGA curves of the complexes[Cu(BBIP)2]Cl2 and [Cu(BBIB)2]Cl2 show no mass loss upto 200°C which indicates the absence of lattice as well as co-ordinated water. The ligand BBIP and [Cu(BBIP)2]Cl2complex exhibit good antifungal and antibacterial activity against Candida albicans and Staphylococcus aureus (gram positive), Escherichia coli (gram negative) respectively, by using modified Kirby-Bauer disc diffusion method. The screening results for anti-proliferative activity for the MCF 7 breast cancerous cells show promising activity. The [Cu(BBIP)2]Cl2 complex has enhanced growth inhibition in a dose-dependent...
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Posted by admin on May 30, 2015 in |
In order to protect from UV radiations, naturally occurring safe and broad spectrum components are desired. The chemical sun blockers viz. ZnO and TiO2 gives broad spectrum UV protection but, its safety is doubted. Aim of present communication was to study UV protective activity of water immiscible pigments of fruit rinds of Garcinia indica. Out of various extracts used for sun protective activity n-butanol, and ethyl acetate extracts of fruit rinds were shortlisted. Both the ethyl acetate and butanolic extracts were fractionated with water to remove the Hydroxy citric acid present in the extract. The ethyl acetate extract after water fractionation exhibited three-fold increase in UV absorbance in both UV A and UV B regions. Fractionated ethyl acetate extract was incorporated in sun protective cream formulation and the Sun protection factor was found to be 3.43 with boot star rating of five. The fractionated ethyl acetate extract of G. indica can be used as natural component for...
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