Posted by admin on Jul 31, 2015 in |
Each company is expected to develop specifications for their products to be accommodated with the inspection guide of pharmaceutical quality control. After product analysis for (X) pharmaceutical company it was noticed that 84.5% of defects were attributed to tablet department while 14.1% and 1.4% for ampoule and syrup departments respectively so it was valuable to solve the tablet department problems. Data was collected for three months for two types of tablets which were analgesic tablet (AT) and digestive enzyme tablet (D.E.T). D.E.T was chosen for further study due to its high percent of defect. By using six sigma methodology to analyze the collected data it was found that the major problem was in tablet microbiological test 85.9% rather than the physical problem 14.1%. D.E.T was subjected to study the variables affecting the bacterial count like raw material, process environment and microbiological analysis accuracy. It was found 66.7 of the problem caused by the variation in process environment. By using factorial design for three variables it revealed that the cleaning...
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Posted by admin on Jul 31, 2015 in |
The present study aimed to evaluate the antitumor efficacy of Snake venom (SV), bee venom (BV), combinations of their components and their apoptotic, cell death mechanisms in liver (HepG2) and breast (MCF7) cell lines. Cytotoxic effects of venoms, L-Amino acid oxidase (svLAAO), phospholipase A2(svPLA2), Melittin ( MEL) were tested against HepG2 and MCF-7 cell lines and IC50 was calculated . mRNA expression of p53, Bax and Bcl-2 was carried out. All the tested compounds had anti proliferative effects on the tumor cell lines with different potency. BV had a higher cytotoxicity against liver and breast cells (IC50=1.26 and 2.85 µg/ml) than SV (IC50=5.86, 13.05µg /ml). The cytotoxicity of svLAAO was much higher than SV with IC50 = 3.65 and 0.48 µg /ml. MEL showed a higher cytotoxic effect than BV against MCF7 cells (IC50=1.14 µg /ml). MEL when combined with svPLA2 gave a synergistic effect on the expression of P53 and Bax in the two cancer cells. Venoms combination had the least synergistic effect relative to other combinations. From...
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Posted by admin on Jul 31, 2015 in |
The bioactive components of Bryonopsis laciniosa fruits have been evaluated using GC/MS. The chemical compositions of the methanolic extract of Bryonopsis laciniosa fruits were investigated using Perkin-Elmer Gas Chromatography –Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis shows that the most prevailing compounds were identified as Oleic Acid, Hexanoic acid, 2-Ethylcyclohexanone, 2-Methyltetracosane, 2-Undecenal, 1,2-Benzenedicarboxylic acid, Ascorbic acid 2,6-dihexadecanoate, Octadecanoic acid, (2E)-2-Decenal.The compounds like Sulfurous acid, n-Nonaldehyde, 2-Hepten-3-ol, Decadienal, 3-Octenoic acid, 1-[2-(acetyloxy)ethyl]-3-oxooctyl acetate $$ acetic acid 3-acetoxy-5-oxo-decyl ester, 9-Octadecenoic acid, 2H-Pyran-2-one, Z,Z-4,15-Octadecadien-1-ol acetate were moderately present. The results confirm the presence of bioactive components which are known to exhibit medicinal value as well as pharmacological...
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Posted by admin on Jul 31, 2015 in |
Larrea divaricata Cav. commonly known as “jarilla hembra” is used in folklore medicine as anti-inflammatory. There was no scientific evidence justifying the medicinal use of the aqueous extract for inflammation diseases. Nowadays, there are two principal types of anti-inflammatory drugs: Steroidal anti-inflammatory drugs (corticosteroids) and non-steroidal anti-inflammatory drugs (NSAIDs), which exert gastrointestinal ulceration and bleeding. In this work the preventive anti-inflammatory activity of L. divaricata aqueous extract (AE) in C3H/He mice was determined by carrageenan- induced inflammation and ear edema tests, also its toxicity on gastric tissues and on blood cells was studied. The chromatographic profile showed: nordihydroguaiaretic acid (NDGA) as majority compound. AE exerted anti-inflammatory action without inducing gastric or blood cell toxicities. Also, it could modulate anti-inflammatory and pro-inflammatory cytokines. NDGA was not the compound responsible of the anti-inflammatory action. This work rescues the potentiality of AE as a gastric and blood cells innocuous anti-inflammatory agent and confirms its ethno- pharmacological...
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Posted by admin on Jul 31, 2015 in |
In today’s hectic lifestyle, such dosage form as plaster, attracts more attention of scientists and gives new opportunities to use it in medicine. The purpose of our study was to investigate consumer properties of transdermal patches, hold the commodity analysis of the dosage form and determine the prospectiveness of their use in modern pharmacotherapy. Market review has been conducted. Analyzed the producing countries of transdermal patches reported on the pharmaceutical market of Ukraine. An analysis of the active ingredients used in the TTS has been carried out. Modern classifications of transdermal therapeutic systems have been given. The approaches to creation of TTS have been investigated. The most important consumer properties of TTS have been...
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