Posted by admin on May 30, 2015 in |
A simple, sensitive, highly accurate method has been developed for the determination of Lafutidine in bulk sample and dosage forms. This method is based on the oxidative coupling reaction of drug with 3-methyl-2-benzothialinone hydrazine hydrochloride (MBTH) reagent in the presence of ferric chloride to form green colored chromogen exhibiting maximum absorption at 630 nm. Beer’s law was obeyed and quantitative results were obtained in the range of 1-18µg/mL. The results of the analysis for the methods have been validated...
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Posted by admin on May 30, 2015 in |
The present study deals with the efficacy of Tribulus terrestris (TT) on aluminium chloride (AlCl3) – induced alterations in the reproductive organs and fertility of the male laboratory mouse. Thirty adult male mice were divided into six groups of five each. Group I served as control while that of II, III and IV received various doses of AlCl3 (25, 50, 100 mg/kgBW/day, respectively), for 30 days. The mice of groups V and VI were administered with TT (100mg/kgBW/day) only and AlCl3 (100mg/kgBW/day) along with TT (100mg/kgBW/day) respectively, for the same duration. Only high dose of AlCl3 (100mg/kgBW/day) – treated mice showed significant reductions in the body weight, sex organs relative weight, sperm count, motility, viability, epididymal sialic acid, seminal vesicular fructose, serum testosterone, antioxidant enzymes (SOD, Catalase, GPx), mating ability and fertility. However percentage of abnormal spermatozoa in the epididymis and level of testicular cholesterol and MDA were significantly increased. Dose-dependent regressive histological changes in the seminiferous tubules and the Leydig cells were also noticed. Supplementation with TT in...
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Posted by admin on May 30, 2015 in |
As a result of tremendous efforts in past few decades, various techniques have been developed in order to resolve solubility issues. However, majority of these techniques offer benefits associated with certain drawbacks; majorly including low drug loading, physical instability on storage and excessive use of environmentally challenging organic solvents. Hence, current effort was to develop an eco-friendly technique using liquid salt, which can offer improvement in dissolution while maintaining long term stability. The liquid salt formulations of poorly soluble model drugs ibuprofen, gemfibrozil and indomethacin were developed using 1-Ethyl-3-methylimidazolium ethyl sulfate (EMIM ES) as a non-toxic and environmentally friendly alternate to organic solvents. Liquid medications containing clear solutions of drug, EMIM ES and polysorbate 20, were adsorbed onto porous carrier Neusilin US2 to form free flowing powder. Liquid loading as high as 70% w/w was achieved while maintaining good flowability and compressibility. The liquid salt based formulations (LSF) demonstrated greater rate and extent of dissolution compared to crystalline drugs. The LSF samples exposed to 40°C/80% RH for 8 weeks,...
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Posted by admin on May 30, 2015 in |
In the present work a simple, accurate and precise method has been developed and validated for the simultaneous estimation of Perindopril Erbumine (PDE), Amlodipine Besylate (AMD)and in their combined dosage form by UV Spectrophotometric Methods. The Method A employs estimation of drugs by simultaneous equation method (SEM) using 298.3 nm and 231.9nm i.e. λmax values of PDE and AMD respectively. Method B employs the estimation of drugs by Absorption Correction method (ACM) at 250.4nm for PDE where absorbance of AMD is zero. Absorbance correction method was based on the property of additivity of absorbances. At 250nm, Perindopril erbumine showed some absorbance while Amlodipine besylate showed zero absorbance. The method involved measurement of absorbance at two wavelengths 250 and 231 nm. For estimation of AMD, corrected absorbance was calculated at 231 nm due to the interference of PDE at this wavelength. Calibration curves were linear with correlation coefficient between 0.999 over the concentration range of 10-150μg/mL and 0.5-10 μg/mL for PDE and AMD respectively. The percent recoveries of the drugs...
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Posted by admin on May 30, 2015 in |
Armodafinil is an enantiopure of the wakefulness -promoting drug Modafinil, unique psycho stimulant recently approved by the US Food and Drug Administration for the treatment of narcolepsy; it is useful for treating excessive day time sleepiness. A simple, sensitive and accurate and economical spectrophotometric method has been developed for the estimation of Armodafinil in bulk and pharmaceutical dosage forms. This method is based on oxidative coupling reaction of 3-methyl-2-benzathiazoline hydrazone (MBTH) in the presence of ferric chloride (Fecl3). An absorption maxima was found to be at 596nm with the solvent system methanol: water (3:97). The drug follows Beer-Lambert law in the range of 10- 50µg/ml with correlation coefficient of 0.999. The percentage recovery of Armodafinil in pharmaceutical dosage form is in between 96- 106. Results of the analysis were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. The proposed method is simple, rapid and suitable for the routine quality control...
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