Posted by admin on Apr 30, 2015 in |
Spices are the potential source of natural products and naturally derived compounds. These compounds show antioxidant, antimicrobial properties. The aim of this study is to evaluate the antimicrobial properties of Cinnamomum zeylanicum (Cinnamon). In this study, antimicrobial activities of 70% ethanolic extracts of Cinnamomum zeylanicum were evaluated against different bacterial strains (E.coli, Pseudomonas aeruginosa, Candida albicans) by agar well diffusion method & MIC determination by broth dilution method. The extract showed a broad spectrum of antimicrobial activities by inhibiting the growth of respective microorganism in Agar well diffusion assay. The present study supports the immense medicinal properties of Cinnamomum zeylanicum. It may be insight for future researches in area of new drug development of herbal...
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Posted by admin on Apr 30, 2015 in |
The objective of present study was to formulate and evaluate Tariquidar bimesylate subcutaneous in situ implants in order to minimize the frequency of doses and toxicity and to improve the therapeutic efficacy. The anthranilic acid derivative, tariquidar (XR9576), is a potent and selective P-gp inhibitor being developed clinically for the treatment of multidrug resistant tumors. Tariquidar bimesylate in situ implants were prepared by polymer precipitation method using two different grades of polymer poly (Lactide -co-glycolide) (PLGA) with three different concentrations. The preparation involves dissolving the biodegradable polymer poly (Lactide -co-glycolide) PLGA 85:15 and PLGA 75:25 in N-methyl-2- pyrrolidone. To this solution drug was added and the polymer drug solution was injected in to the aqueous mediato forma solid implant. Based on initial burst release, optimized formulations were selected and evaluated for surface morphology, in vitro drug release and accelerated stability studies. It was observed that the formulations with more polymer concentration and in combination with polyethylene glycol(PEG) and Benzy l benzoate (BB) showed increased entrapment efficiency and exhibited moderate...
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Posted by admin on Apr 30, 2015 in |
The objective of present study is to develop and validate a method for the estimation of sucralfate and oxetacaine in suspension dosage form. An isocratic reversed phase high performance liquid chromatographic method was developed to estimate sucralfate and oxetacaine using thermohypersil BDS (4.6 x 150mm, 5). The mobile phase was prepared by mixing ammonium acetate (pH- 4.4) and methanol in the ratio of (60:40 v/v) which is run isocratically at the flow rate of 0.8ml/min. the wavelength at which detector (PDA) was set is 263nm. The retention time of oxetacaine and sucralfate is 2.08 and 3.4 minutes. Linearity was observed in the concentration range of 20-60g/ml for oxetacaine and 1000-3000g/ml for sucralfate. The percent recovery of sucralfate and oxetacaine was found to be 100. The limit of detection of oxetacaine and sucralfate is 0.178 g/ml and 2.93 g/ml and the limit of quantification of oxetacaine and sucralfate is 0.541 g/ml and 8.879 g/ml. The formulation was subjected to acid, base, peroxide, sunlight and heat and stressed samples were analysed...
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Posted by admin on Apr 30, 2015 in |
Eurycoma longifolia has been widely used by society as an aphrodisiac especially in Indonesia, Vietnam and Malaysia. However, only few studies have been published related to the herb-drug interaction. Previous study showed that the standardized extract of E. longifolia affected phase I rosiglitazone metabolism especially in the old male rat hepatocytes for both diabetic and normal rats. The present study evaluated the possible mechanism of E. longifolia extract in affecting of phase I rosiglitazone metabolism in the old normal male rat hepatocytes. Experiment was performed used ten cellular stimulants/inhibitors. Old normal male rats were divided in ten groups (n = 6) and the hepatocytes were isolated using perfusion technique. Each group had a negative control (in the presence of vehicle), a positive control (in the presence of cellular inhibitors/stimulants) and 6 test subgroups (in the presence of cellular inhibitors/stimulants and 0.001-100µg/mL of E. longifolia extract). Effect of E. longifolia extract on phase I rosiglitazone metabolism was determined by colourimetric method of Nash at 415nm. The results showed the significant...
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Posted by admin on Apr 30, 2015 in |
Studies on plant secondary metabolites have been increasing over the last 70 years. These molecules are known to play a major role in the adaptation of plants to their environment, but also represent an important source of active pharmaceuticals. Role of primary metabolites in basic life functions such as cell division and growth, respiration, storage, and reproduction have been described in modern chemistry and biology. In biology, the concept of secondary metabolite can be attributed to Kossel. He was the first to define these metabolites as opposed to primary ones. In the present study, the bioactive secondary metabolite myricetin was identified and isolated from in vivo and in vitro tissue of Cochlospermum religiosum a critically endangered medicinal plant. Myricetin is a naturally occurring flavonol found in many plants like grapes, berries fruits, vegetable, herbs as well as other plants. Myricetin has wide array of biochemical properties, such as anti-neoplastic, anti-carcinogenic antioxidant activity, anti-inflammatory effects. Myricetin was isolated and identified from leaf and callus by using different techniques such as...
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