Posted by admin on Jun 30, 2015 in |
The present study has been designed to evaluate the bioactive compounds and cytotoxic activity from the n-hexane fraction of methanolic extract of Antigonon leptopus flowers. The n-hexane fraction was analysed by GC-MS (Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II) which revealed the presence of many diversified compounds including flavonoids, Vitamin D2 derivative, carbohydrates, amino acids, quinazolines etc. All the compounds were identified from Wiley spectral library. Mass spectral data of 16 compounds is presented. The in vitro cytotoxic activity of n-hexane fraction of the methanolic extract was performed by MTT assay method against CHOK 1cell line (Chinese Hamster Ovary cell line) and A-549 cell line (Human Lung adenocarcinoma epithelial cell line).Under the experimental conditions the cytotoxic property was not observed for n-hexane fraction. From the results it is evident that Antigonon leptopus flowers contain various medicinally active phytocomponents .The absence of cytotoxic activity against CHOK 1cell line and A-549 cell line recommends the safety of the Antigonon leptopus...
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Posted by admin on Jun 30, 2015 in |
The aim of the present work was to investigate the phytochemical components of an antidiabetic polyherbal formulation. The formulation was prepared using ingredients of Azadirachta indica A. Juss, Commiphora mukul, Curcuma longa L, Emblica officinalis Gaertn, Garcinia cambogia Gaertn, Gymnema sylvestre Retz, Momordica charantia L, Ocimum sanctum L and Trigonella foenum-graecum L. The formulation was evaluated for phytochemical properties by following standard protocols. The colour intensity or the precipitate formation was used as analytical responses to these tests. The formulation revealed the presence of active phytoconstituents such as carbohydrates, amino acid, proteins, phenols, flavonoids, tannins, terpenoids, steroids and insulin. The HPTLC fingerprint analysis revealed sixteen bioactive compounds when scanned at 520 nm and thirteen bioactive compounds at 254 nm. The study confirms that the formulation contains many phytoconstituents making it a prospective candidate for further...
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Posted by admin on Jun 30, 2015 in |
Strychnos potatorum Linn. is a medium sized deciduous tree belonging to the family Loganiaceae. The collected seeds of Strychnos potatorum were dried and powdered. Methanolic extract of these seeds was prepared by cold maceration. The preliminary phytochemical screening of the crude extract revealed the presence of flavonoids and phenolic compounds. The crude extract was partitioned (gradient) with different solvents like chloroform, ethyl acetate, acetone and water. After conducting preliminary in vitro DPPH free radical scavenging assay on these fractions, the one with maximum antioxidant activity (bioactive fraction) was selected and screened with different in vitro antioxidant methods. The different in vitro antioxidant methods include DPPH free radical scavenging assay, nitric oxide radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay and inhibition of peroxide formation method. The percentage inhibition of free radicals in each method was compared with standard drug. Ascorbic acid and α- tocopherol were used as standard drugs. The seeds of Strychnos potatorum have shown significant antioxidant...
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Posted by admin on Jun 30, 2015 in |
Pulsatile system is gaining a lot of interest as it increases patient compliance by means of providing time and site specific drug delivery system. This work will aim at the design of a sustained release formulation with potential use of metoprolol tartrate capsule which is used in the treatment of hypertension. The design involves the preparation of cross linked hard gelatin capsules by using formaldehyde, then the drug diluents mixture loaded in, which was separated by using hydrogel plugs of different polymers, such as, HPMC 15cps, HPMC 50cps, HPMC 100cps, HPMC K4M, HPMC K15M, HPMC K100M, xanthan gum, carbomer 940, carbopol 971, sodium CMC and sodium alginate of two different amounts (75mg and 100mg respectively). Prepared formulations were subjected to evaluation of various phycal parameters and in vitro drug release studies. From the in vitro dissolution studies it was found that by increasing the amount of polymer, release rate was decreased. 75mg of HPMC 15cps showed 91.23% drug release in 5hr whereas 100mg showed 96.68% drug release in 7hr....
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Posted by admin on Jun 30, 2015 in |
In present study the electro-oxidative behaviour and determination of meropenem at a glassy carbon electrode were investigated using cyclic, differential pulse anodic adsorptive stripping voltammetry (DPAAdSV) and square wave anodic adsorptive stripping voltammetric (SWAAdSV) techniques. The dependence of the peak current and peak potentials on pH, concentration and scan rates were examined. The oxidation of meropenem gave a well-defined single irreversible peak at glassy carbon electrode vs Ag/AgCl (3M KCl) reference electrode. The oxidation process was diffusion controlled. A linear response was obtained between 1.0 x10-6to 4.0x 10-5 M in aqueous media for differential pulse and square wave techniques. The recovery studies were also achieved to check sensitivity and selectivity of the proposed methods. The proposed methods were applied for the determination of meropenem from pharmaceutical dosage forms without any time-consuming extraction, separation, adsorption steps. The value of LOD was achieved 1.27×10-7 M and 2.24×10-7 M for DPAAdSV and SWAAdSV...
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