Posted by admin on Apr 30, 2015 in |
A very sensitive, rapid and simple Ultra High Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) method has been developed and validated for the simultaneous estimation of Metformin Hydrochloride and Sitagliptin Phosphate in standard and tablet formulation. The chromatographic separation of the drugs was achieved on a Hypersil Gold C18 analytical column (150x3mm, 5µ) with an isocratic elution of methanol and water with 0.2% formic acid (48:52 v/v) at a constant flow rate of 500µL/min on a UHPLC and the retention time of Metformin Hydrochloride and Sitagliptin Phosphate were 1.40±0.01 and 2.38±0.01 min respectively. Elutes were detected online on an Orbitrap mass spectrometer in ESI (+) mode. The method was validated according to ICH guidelines. The calibration plot was linear with correlation coefficient (r2) of 0.9998 for Metformin Hydrochloride in the concentration range of 0.04-0.28ng/mL and 0.9992 for Sitagliptin Phosphate in the concentration range 0.04-0.28 ng/mL respectively. The LLOD of Metformin Hydrochloride and Sitagliptin Phosphate were 0.2 and 0.02pg/mL respectively. This method was validated with respect to linearity, accuracy, precision, specificity and...
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Posted by admin on Apr 30, 2015 in |
Empirically dry fallen of Artocarpus altilis leaves are widely used as a traditional medicine, but the green leaves that have been widely studied. Therefore, this research was conducted to compare the metabolite profile of green leaf, attached yellow, fallen yellow, fallen dry, and fermented green leaves. Comparison of flavonoid content was conduted by HPLC. Extraction was performed using Soxhlet apparature, fractionation by VLC and setrifugal chromatography. HPLC analysis of various leaves showed that the fallen dry leaves contained the highest flavonoid compound. Three compounds were isolated, compound 1 characterized Uv-Vis, IR, 1H-NMR, 13C-NMR spectroscopies and identified as sitostenone. Compound 2 showed UV spectrum typical of flavonoid with M – 392 and tentatively identified as flavon with two prenyl substituents. Coumpound 3 was identified as b-caroten based on its UV spectrum and chromatographic...
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Posted by admin on Apr 30, 2015 in |
Background: Vitamin D3 deficiency continues to be an unrecognized epidemic in many populations around the world. Vitamin D is important for the absorption of calcium, and bone formation and maintenance. Objective: This study was carried out with an objective of evaluating the prevalence of low Vitamin D levels (insufficiency and deficiency) in female office workers and housewife’s in Geetanjali medical college and hospital, Udaipur. Material & method: This study was carried out in Geetanjali hospital and medical college, a retrospective study conducted on 50 working and 50 housewife’s subjects between age group 18-32 years. Body mass index (BMI) was calculated. Serum calcium, serum phosphorous, and alkaline phosphatase were determined by a fully-auto-analyzer. The serum level of Vitamin D3 was measured using the electro chemiluminescence immunoassay methodology. Results: The difference in the BMI between the office workers and housewife’s was statistically no significant. Vitamin D3 was 18.72 ± 6.97ng/ml (SEM0.987) in office worker’s and 9.94 ± 6.14 ng/ml (SEM 0.869) in housewife’s with a highly significant (p<0.0001) statistical difference. Conclusion: ...
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Posted by admin on Apr 30, 2015 in |
The electrochemical behavior of Ketamine is studied by employing D.C. Polorography (DCP), Cyclic Voltammetry (CV) and Differential Pulse Polarography (DPP) in universal buffers of pH ranging from 2.0-12.0. The kinetic parameters, such as transfer coefficients, diffusion coefficients and heterogeneous forward rate constant values were evaluated by employing these techniques. Differential Pulse Polarography was employed for the estimation of ketamine in selected pharmaceutical...
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Posted by admin on Apr 30, 2015 in |
Oral salbutamol sulphate has site-specific absorption in the stomach and upper part of the small intestine. Its bioavailability is about 40% due to several factors including narrow absorption window and extensive intestinal metabolism. The aim of this study was to formulate and optimize sustained release floating tablets of salbutamol sulphate in order to improve its bioavailability and reduce its dosing frequency. Accordingly, floating tablets were prepared by wet granulation technique and drug release analysis was performed by HPLC. The effects of polymer level, polymer type (XG or HPMC), polymer ratio (XG/HPMC; 1:1, 1:3, 3:1) and NaHCO3 level on floating lag time, floating duration, cumulative release within 1 hr, and release rate were investigated. From preliminary studies, the polymer with 1:3 (XG:HPMC) ratio and NaHCO3 were selected as significant factors and cumulative release at 1 hr and release rate were chosen as significant responses, respectively. Hence, the effect of these factors were further studied and optimized by central composite design. The most desirable representative optimal point was obtained at 24.79%...
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