Posted by admin on May 30, 2015 in |
Many countries in Africa, Asia and Latin America use traditional medicine (TM) to meet some of their primary health care needs. In Ethiopia, Traditional Medicine (TM) is a part of the national heritage. Also Ethiopia is the home of many nationalities and remarkably diverse flora, including numerous endemic species that are utilized in the different traditional medical practices. Beyond the convenience, traditional home remedies have found favour with a public that wants to take a more holistic approach to its ailments. This study sought to assess the traditional home remedy usage among people in Harar, Ethiopia. The present survey covered the people residing in Kebele 6, Jugal, Harar, Ethiopia. A structured interview questionnaire based on traditional home remedy usage was distributed and face-to-face interviews were conducted. Collected data were tabulated. Among the 338 (100%) respondents, 150 (44%) were males and 188 (56%) were females. 63 (19%) respondents were between the age group of 20 – 25 years, 41 (12%) were between 26 – 30 years, 66 (19%) were between...
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Posted by admin on May 30, 2015 in |
The aim of this study was to determine the anti-microbial and anti-adhesive properties of a Lactobacillus and Bacillus, against several micro-organisms, including Gram-positive and Gram-negative bacteria. Biofilm producing micro- organisms were isolated from different samples. The isolated micro-organism were characterized and identified. The anti-microbial and anti-adhesive activities were determined. The bacteroicin production was quantified and was observed. The maximum bacteroicin production was observed among various isolated micoorganisms. The bacteroicin produced by the Lactobacilli and Bacilli showed antimicrobial activity against pathogenic micro-organisms (including Pseudomonas, Escherichia col, Staphylococcus, Aspergillus and Salmonella typhi), the minimum inhibitory concentration (MIC) were achieved for bacteroicins the most. The antagonistic characteristics of bacteriocin were studied by agar well diffusion method. Furthermore, the micro- organism showed anti-adhesive activity against most of the micro-organisms evaluated. Biofilm production was observed and quantified among various biofilm producing isolated micro-...
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Posted by admin on May 30, 2015 in |
The Ooctapéptido (LAN: 3-(2-Naphthalenyl)-D-alanyl-L-cysteinyl-L-tyrosyl-D-tryptophyl-L-lysyl-L-valyl-L-cysteinyl-L-threoninamide (2 → 7) disulphide acetate, angiopeptin acetate) cyclic covered gold nanoparticles (AuNPs) results in a complex (AuNPs-LAN) when reacted following the aqueous synthesis using Turkevich method for preparing colloidal gold nanoparticles followed by reaction of conjugation with the octapeptide LAN. The nanoconjugate was characterized by transmission electron microscopy high-resolution, UV-vis spectrophotometry, and by Zeta-potential. The objective of this study was to develop a method of capillary zone electrophoresis (CZE) to determine AuNPs and AuNPs-LAN in vitro. The reaction was I carried out by reduction of HAuCl4 with trisodium citrate, based on the synthesis of Turkevich-Frens-Kimling for colloidal Au at 90 °C where the plasmon resonance was detected at a wavelength of 526 nm, previously reported from 1954, 1976 and 2006, wavelength UV-vis nanoconjugate was 531 nm in which prolongation observed in migration times. We also describe part of the validation (linearity and precision) by capillary electrophoresis, arguing that it is a suitable method to quantify the gold nanoparticles and AuNPs-LAN complex. We conclude that the...
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Posted by admin on May 30, 2015 in |
Antioxidants prevent the damage done to the cells by free radicals that are released during the normal metabolic process of oxidation. Some of these free radicals include reactive oxygen species, hydroxyl, the superoxide anion, hydrogen peroxide and peroxyl which generate metabolic products that attack lipids in the cell membranes or DNA. These are associated with DNA damage, carcinogenesis and cellular degeneration related to aging and also contributes to heart diseases and arthritis. Researchers around the world have identified many natural constituents with antioxidant activity. Thus in the present work we have studied the synergistic antioxidant activity of lipoic, ferulic and ellagic acid which is naturally occurring compounds having medicinal properties by 1, 1-diphenyl-2-picrylhydrazyl assay. Highest antioxidant activity is reported for mixture of all the three compounds with 98% & 64% inhibition in both methanoilc and aqueous solutions followed by ferulic acid with 97% & 70% inhibition and mixture of ellagic and lipoic acid with 96% & 59% inhibition in both methanoilc and aqueous solutions. Thus from the results it...
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Posted by admin on May 30, 2015 in |
A simple, precise, specific and accurate high-performance thin-layer chromatographic method has been developed for the simultaneous determination of Emtricitabine (EMB) and Tenofovir (TEN) in pharmaceutical dosage form. The separation was carried out on Merck HPTLC aluminum plates of silica gel 60 F254, (20×10 cm) with 250 μm thickness using toluene: ethylacetate: methanol in the ratio (2:8:1 v/v/v) as mobile phase. HPTLC separation of the two drugs followed by densitometric measurement was carried out in the absorbance mode at 272 nm. The drugs were resolved satisfactorily with Rf values of 0.44 and 0.68 for EMB and TEN, respectively. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.9999 and 0.9995 for EMB and TEN, respectively, in the concentration range of 40–400ng spot−1 for EMB and 200–700ng spot−1 for TEN. The method was validated for precision, robustness, specificity and accuracy. The limits of detection and quantitation were 20 and 40ng spot−1, respectively, for EMB and 100 and 200ng spot−1, respectively, for TEN. The proposed...
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