Posted by admin on Jun 30, 2015 in |
Lysosomes of mammalian tissues contain a number of cysteine proteinases such as Cathepsins B, H and L, which participate in intracellular protein degradation and are involved in various pathological disorders. These proteinases have been purified to ~ 1600 fold with ~25% yields from goat liver. These Cathepsins having similar molecular weight (~25,000 Da) were obtained as a single pool after initial purification steps i.e. homogenization of acetone powder, acid autolysis at pH 4.0, 30-70 % (NH4)2SO4 fractionation and molecular sieve chromatography on Sephadex G-100, were simultaneously separated and purified on exchange chromatographies. Cathepsin L was completely separated and purified from Cathepsin B & H at CM- Sephadex C-50 column at pH 5.6 and was eluted as bound protein at 0.62 M NaCl with a purification fold ~1629 and 22 % yield. Cathepsin B was eluted as bound protein in ~1545 fold with ~45% yield at 0.40 M concentration. Complete separation of Cathepsin H from Cathepsin B was achieved on DEAE-Sephadex A-50 at pH 6.0 where the former was obtained...
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Posted by admin on Jun 30, 2015 in |
Present era meant for utilization of natural resources in the development of dosage forms. In the present investigation natural biodegradable polymer olibanum resin is employed to develop matrix tablets of ambroxol hydrochloride for sustained release. Various Ratios of resins is study its ability to retard the early release of the drug from formulation. The tablets were prepared by wet granulation technique and were subjected for their thickness, weight variation test, drug content, hardness and friability. The in vitro dissolution studies were carried out using USP apparatus type II (paddle) at 100 rpm. Drug content of the formulations ranged between 97.78±0.08 to 100.12, where as hardness varies from 5.9-7.6kg/cm2. From the in vitro release studies it was found that F5 formulation was similar to marketed sample hence it was selected as a optimized formulation. Higuchi release kinetics shows r2=0.9298, from Korsemeyer- Peppas plot n value >0.89 indicates super case II type of release indicating that the drug release from these formulations is by erosion of polymer. The optimized formulation of...
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Posted by admin on Jun 30, 2015 in |
A brief study to analyze oxidative stress and antioxidant response of cadmium stress was carried out. The plant chosen Vigna radiata (var. SML-668) was hydroponically grown in 0, 50µM, 100µM and 150µM, cadmium chloride. The roots were taken from hydroponic culture at 6 days of growth for various enzymatic and metabolic estimations. Among the antioxidant enzymes, root catalase and ascorbate peroxidase decreased while guaiacol peroxidase and superoxide dismutase increased in response to cadmium. The lipid peroxidation and protein oxidation too increased probably due to production of active oxygen species by heavy metal stress which causes lipid peroxidation. So, Cd was definitely toxic to root system of V. radiata plants as indicated by consistent increase in peroxidation and protein oxidation. However, the plant was able to counter this partially by concomitant increase in SOD and guaiacol peroxidase. But, the other two antioxidant enzymes catalase and ascorbate peroxidase could not counter oxidative stress generated by Cd as they were...
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Posted by admin on Jun 30, 2015 in |
Phytochemicals from several herbaceous and spice plants have been reported to have defense properties against various diseases. Potential of some of such plants like Allium sativum, Curcuma longa, Boerhaavia diffusa, Pterocarpus marsupium and Zingiber officinale in cancer therapy has already been proved. The state of Kerala in southern India hosts large number of medicinal herbs and is also referred as a hotspot of biodiversity. The current study aimed at computational screening of potent lead molecules from ten selected medicinal plants and five spices widely used in Kerala, which are rich in biological diversity and its traditional values. Even though, the anticancer properties of all these plants and spices were reported, their mode of action and target molecules is not well explained. Hence, the interaction of these compounds with anti-apoptotic proteins Bcl-2 and Bcl-XL are studied and discussed in this paper. The -CDOCKER energy, -CDOCKER interaction energy, Hydrogen bonds, binding energy, and complex energy were predicted through molecular docking studies. Based on binding energy and CDOCKER scores, the active components...
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Posted by admin on Jun 30, 2015 in |
Larrea divaricata Cav. (Zygophyllaceae) is an autochthonous plant of South America, widely distributed in Argentina. It is used in folk medicine as an anti-inflammatory drug. By other way, presents well documented antitumoral and immunomodulatory effects. Nordihydroguaiaretic acid (NDGA), an antioxidant compound, had been previously described in this plant. Nowadays a formulation for hair growth based on an extract of L divaricata is commercialized in Argentina. Taken these into account, the objective of this work was to determine the systemic and local toxicities of the aqueous extract of L divaricata, by giving it under oral and topical administration. Also, it was proposed to analyze the participation of NDGA in the systemic toxicity of the plant extract. The extract presented low acute toxicity, and the histopathological examination of the tissues did not show any signal of toxicity at the same time that did not affect biochemical parameters except eosinophils which were decreased by the treatment. Which is more; the extract did not exert dermatological or ocular irritation. It is important to...
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