Posted by admin on Jun 30, 2015 in |
A simple, sensitive, precise, accurate and cost effective reverse phase high performance liquid chromatographic method for the simultaneous estimation of bupropion HCl and naltrexone HCl from a single formulation was developed and validated in accordance to ICH guidelines. The two drugs were eluted under isocratic mode using a 250 x 4.6 mm i.d, 0.45 µm Phenomenex Chromosil C18 column. The mobile phase was composed of a mixture of methanol, acetonitrile and water in the ratio of 60:20:20 v/v (pH adjusted to 4.8) at a flow rate of 1.0mL/min. The limit of detection and limit of quantification were found to be 0.5μg/mL and 1.7μg/mL, for both the drugs. The label claim of the combined dosage form was in good agreement with the experimental assay amounts; 99.5% and 99.7% for bupropion HCl and naltrexone HCl, respectively. This method can be successfully applied for the simultaneous estimation of bupropion HCl and naltrexone HCl combination for routine quality control...
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Posted by admin on Jun 30, 2015 in |
Peroxidase enzyme plays an important role in pharmaceutical, Industrial and various research laboratories. This enzyme can be purified by various different method. Our research trend was to purify the enzyme in a convenient and easy way. For this purpose a new affinity column was prepare by immobilizing PABA to N-HydroxySuccinamaideagarose gel. The presence of peroxidase was investigated on various Bangladeshi vegetables, such as, daikon, cabbage, cauliflower, tomato and sweet potato. Based on a previous screening experiment, daikon contains the highest amount of peroxidase. The juice of daikon was first extracted by tincture press and subjected to ammonium sulphate precipitation. The precipitate was dissolved in 25 mM phosphate buffer, pH7.4 containing 500 mMNaCl, 1 mM MnCl2 and 1 mM CaCl2. The crude extract thus obtained was subjected to an inhibitor affinity chromatography (IAC) method. Here, we have taken advantage of the affinity of peroxidase toward p-aminobenzoic acid hydrazide (PABAH) and subsequent recovery of the peroxidase by a known destabilizing agent ascorbic acid. The partially purified sample was applied to the...
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Posted by admin on Jun 30, 2015 in |
The acaricidal activity of leaf extracts of Annona squamosa, Azadirachta indica and Calotropis procera were tested against the cattle ticks under in vivo condition. Plants were extracted with methanol and water and the extracts were tested against the cattle ticks under in vivo condition. The aqueous and alcoholic extracts of A. indica showed maximum mortality rate of ticks followed by A. squamosa and C. procera when tested individually. In combination of plant extracts, hot water extracts of dried leaf powder showed 100% mortality of ticks on 5th day whereas ethanol and methanol extracts showed 83% and 80% of mortality respectively. Based on the above experimental results, it is confirmed that the selected plant materials possess more acaricidal activity against cattle ticks. Plant extracts in combinations are more effective than single drug...
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Posted by admin on Jun 30, 2015 in |
The present study report deals with the medicinal plants used by the irular tribal people of Coimbatore against pyogenic infections. Five different plants like Abutilon indicum, Datura metel, Lantana Camaro, Tridax procumbens and Leucas aspera were collected and their antimicrobial activity were tested against bacteria such as Staphylococcus aureus, Streptococcus pyogenes, Bacillus anthracis, Pseudomonas aeruginosa, Escherichia coli, Shigella dysenteriae, Salmonella typhi, Klebsiella pneumonia, Proteus vulgaris and fungi such as Aspergillus niger, Mucor rouxii, Fusarium solani, and Penicillium chrysogenum. All the plants showed antimicrobial activity. Among these Abutilon indicum, Datura metel, Lantana Camaro showed good zone of inhibition against the test organisms. Datura metel showed high zone of inhibition. The extracts of Datura metel showed lowest Minimal inhibitory concentration. Phytochemical analysis showed the presence of alkaloids, flavonoids, saponins and...
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Posted by admin on Jun 30, 2015 in |
The present study a simple, accurate, precise and cost effective UV-Visible spectrophotometric method for development and validation of folic acid as vitamin. folic acid is a water soluble vitamin, so solvent used throughout the experiment was chloroform, butanol,0.1N NaoH, water .the absorption maxima of drug was found at 250nm . Beer’s law was obeyed in the range of 5ppm-30ppm. The developed method was successfully validated with respect to linearity, accuracy and precision. The method was validated and shown linearity in mentioned concentration. The percentage relative standard deviation of inter-day and intra-day precision range 45.15%. Hence proposed method was precise, accurate and cost effective, simple and rapid. This validated method can be applicable for quantitative determination of the titled drug with respect to assay for solid dosage...
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