Posted by admin on Mar 30, 2015 in |
In this study, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were performed on a series of 73 experimentally reported natural flavonoid derivatives as AMV and HIV Reverse transcriptase (RT) inhibitors with pIC50 values ranging from 4.00 to 5.92. Based on alignment, highly predictive CoMFA and CoMSIA were obtained with cross-validated q2value of 0.760 and 0.696 respectively, and non-cross-validated partial least-squares (PLS) analysis with the optimum components of five showed a conventional r2 of 0.964 and 0.977 respectively. The CoMFA and CoMSIA models have been further validated for their stability and robustness using group validation and boot-strapping techniques and for their predictive abilities. The analysis of CoMFA contour maps provided insight into the possible modification of the molecules for better...
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Posted by admin on Mar 30, 2015 in |
Low solubility and biovariability associated with cilostazol (CLZ) pose serious formulation problems despite several advancements in dosage form design. The present research work amalgamates two different solubility enhancement techniques, viz. spray drying and cyclodextrin inclusion complexation to produce amorphous composites of CLZ with increased solubility and dissolution characteristics. FTIR studies unveiled inclusion/ interaction of cyclohexane ring and tetrazole moiety of CLZ in the hydroxypropyl-beta-cyclodextrin (HP-β-CD) cavity. Thermal analysis by DSC showed a decrease in melting points of CLZ and HP-β-CD, indicating favorable complex formation. P-XRD studies revealed reduction in crystallinity and amorphization of CLZ in the complex. SEM indicated a drastic change in surface morphology compared to pure CLZ. These complexes were compressed into oral disintegrating tablets (ODTs) using direct compression technique. The ODTs were characterized for disintegration time, wetting time and in vitro dissolution. Remarkable improvement in solubility and in vitro dissolution characteristics was observed for ODTs in water and compendial dissolution media. Inclusion complexation with HP-β-CD, amorphous composites produced by spray drying technique and the use of...
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Posted by admin on Mar 30, 2015 in |
Present study was carried out to assess testicular toxicity of pyrethroid insecticide, Deltamethrin (DM) in male Swiss albino mice, Mus musculus and to evaluate protective role of Allium sativum (AS) in alleviating the detrimental effect of DM. Forty male mice were divided into five experimental groups (8 mice per group) and were categorized : Control- Given distilled water and food ad libitum, Vehicle Control (oil)- Given peanut oil, DM treated- Given 1/5th of LD50 (6 mg/kg body weight) of DM dissolved in peanut oil via oral gavage, AS-given Allium sativum only (200 mg/kg body weight), DM + AS- given Deltamethrin along with Allium sativum (6 mg/kg body wt. + 200 mg/kg body wt.). Experiment was conducted for a duration of 45 days. DM caused a significant reduction in body weight, testicular weight, Total Protein, Superoxide Dismutase (SOD), Catalase (CAT), Glutathione (GSH), Glutathione Peroxidase (GPx), Glutathione Reductase (GRx), Glutathione-S- Transferase (GST) and Glycogen content of testis. DM-treated group also showed a significant increase in testicular malondialdehyde (MDA) and Cholesterol concentrations....
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Posted by admin on Mar 30, 2015 in |
The main purpose of the study was to examine the effect of QoL of PHN patients after the treatment of tramadol (50mg-200mg), with topical cream containing 3.33% doxepin and 0.05% capsaicin and clinical correlations with CYP2D6 polymorphism. The study was prospective, non-responders versus responders, conducted in 246 outpatients of PHN undergoing tramadol treatment for 4 weeks. Rescue analgesia given in the form of the topical cream consisting of the combination of 3.33% doxepin and 0.05% capsaicin to the affected areas of patients along with tramadol therapy. The QoL as per WHO QoL BREF Questionnaire was evaluated in all the study participants. All samples were analyzed for CYP2D6 (*2,*4 and *10) polymorphism using PCR-RFLP method. Although the QoL score showed magnitude of improvement was higher in responders as compared to non-responders. Insignificant interactions were found in all domains of QoL with respect to CYP2D6*2 and CYP2D6*4 polymorphism. However, a significant (p<0.001) interaction was found in CYP2D6*10 polymorphism with respect to environmental domain of QoL. A significant interaction was found with...
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Posted by admin on Mar 30, 2015 in |
Cervical cancer (CaCx) is the third most common female cancer worldwide with an estimated 5, 27, 624 new cases and 2, 65, 653 deaths in 2014. We conducted a systematic review and meta- analysis to assess safety of prophylactic HPV vaccines compared against placebo. Four unique trials were included out of 607 publications. The total patient data evaluated was 1427(852 in vaccinated arm; 575 in the placebo arm). The pain was the major local side effects with 61% followed by swelling 22% and redness 17% in the vaccinated arm and similarly in the placebo arm pain 72% followed by swelling 18% and redness 10%. Headache was the most frequently experienced systemic side effects 42% in vaccine and placebo group. Unsolicited adverse event in vaccinated and placebo group were 39% and 37% respectively. New onset chronic disease in vaccinated and placebo arm were 3% and 2% respectively. Serious adverse event were 3% in vaccinated and 2% in placebo group. Undesirable pregnancy outcomes were same for both vaccinated and placebo arm...
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