Posted by admin on Jul 31, 2015 in |
L-asparaginase is responsible for the hydrolysis of L-asparagine into L-aspartic acid and ammonia. L-asparaginase is used as an effective therapeutic agent against lymphocytic leukemia. It also finds applications in food industry. Though much has been unraveled about LA, it may appears today that it is at the tip of iceberg and there is tremendous scope in screening of novel LA sources and studying their properties and applications. In the current study L-asparaginase producing bacteria were screened from forest soil of Vasai, MS, India. Modified M9 medium was used for screening the L-asparaginase producers. Out of the total 885 organisms screened for L-asparaginase production, one isolate was found to produce substantial L-asparaginase (15.94 IU/ml). This isolate was further identified by 16S ribosomal RNA gene sequencing as a novel strain Salinicoccus sp. M KJ997975. This orange pigment producing isolate was found to be moderately halophilic and alkaliphilic. Not much literature is available on L-asparaginase producing gram positive alkalophilic and halophilc bacteria and this is the first kind of report on L-asparaginase...
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Posted by admin on Jul 31, 2015 in |
The contents of Sodium lauryl sulphate (SLS) and Polysorbate (Tween 80) were studied in different formulations of Nebivolol film coated tablets. The contents were extracted using the diluent Methanol: Water in the ratio 60:40 v/v and analyzed in HPLC coupled with Evaporative light scattering detector (ELSD). SLS and Tween 80 are solubilizing agents and the amount of these contents are critical in formulations as they are play a major role in the release pattern of the drug for in-vivo and in-vitro dissolution profiles. A reverse phase liquid chromatographic (RP-LC) method was developed for the quantification of Sodium lauryl sulphate (SLS) and Polysorbate (Tween 80). The method was optimized using buffer (prepared by dissolving 0.77g of Ammonium acetate taken in 2000mL milli-Q-water and then pH was adjusted to 4.5with dilute acetic acid solution) as mobile phase-A and Acetonitrile mobile phase-B. The flow rate was set at 1.0 mL min-1 and the column temperature was maintained at 40°C. The capability of specific method developed was demonstrated by studying the validation parameters...
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Posted by admin on Jul 31, 2015 in |
Present aim to study the biochemical composition, i.e., protein, carbohydrate, lipid, ash, amino acids, fatty acids, vitamins, minerals and Carbon, Hydrogen, Nitrogen from Skeletonema costatum. The biomass of marine diatom (S.costatum) were collected from Parangipettai, Southeast cost of India. The lyophilized powder of S.costatum was used for biochemical analysis. The results of proximate composition showed that the percentage of protein was (12.12%), (carbohydrate 5.94%), (lipid 1.567%) and ash (2.96%). Totally 10 essential and non-essential amino acids were analyzed (24.49mg/g – 22.36 mg/g). The saturated mono unsaturated and poly unsaturated fatty acids was (14.86%), (4.25%) and (10.86%). The vitamins and mineral content was 384.19 mg/g – 459.64 mg/g respectively and 1.627 microgram dry powder of Skeletonema costatum contained the ratio of Carbon (15.04%), Hydrogen (2.59%) and Nitrogen (3.17%) were present. The results of this research showed that S.costatum contained valuable nutritional values and we suggest that in future research on S.costatum can be use food for...
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Posted by admin on Jul 31, 2015 in |
The aim of present work was to develop and evaluate solid lipid nanoparticles (SLNs) based gel for topical delivery of antifungal agent. Bifonazole (BFZ) is an imidazole antifungal agent having broad spectrum activity against dermatophytes, moulds, yeasts, fungi and some gram positive bacteria. BFZ SLNs systems were developed by melt emulsification followed by ultrasonication technique using Precirol ATO5 as a solid lipid and Tween 80 as a surfactant. Developed SLNs were evaluated for particle size, polydispersity index (PI), entrapment efficiency (EE) and drug release profiles. Process and formulation parameters were optimized. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) studies were carried out on SLNs to mark the changes in the drug and lipid modifications. The BFZ SLNs based gels were prepared using Carbopol 940 as a gelling agent. The SLNs based gels were evaluated for rheological parameters, in vitro drug release and permeation studies. In vitro antifungal study suggested that the SLNs based gel was more effective in inhibiting growth of Candida albicans. Thus the study concludes that...
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Posted by admin on Jul 31, 2015 in |
Aim: To comparatively evaluate the antihypertensive efficacy and tolerability of ramipril and telmisartan in stage 1 hypertensive diabetic patients. Material and Methods: This short term, prospective, randomized, interventional, comparative clinical study was conducted amongst 53 patients of stage 1 hypertension with type 2 diabetes (27 ramipril and 26 telmisartan patients), aged 30 years and above. Ramipril was administered 5 mg/day and telmisartan 40 mg/day respectively throughout the study period of 3 months. Blood pressure was measured using mercury sphygmomanometer. Paired and unpaired student t-test was applied. Results: Mean SBP was 153.2 and 154.52 mm Hg and mean DBP 87.6 and 87.57 mm Hg in ramipril and telmisartan group respectively. During follow-up the mean SBP and DBP consistently decreased. At 3 months the mean SBP was 124.72 and 123.83 mm Hg in ramipril and telmisartan group respectively. Similarly, the mean DBP at 3 months was 78.16 and 76.7 mm Hg in ramipril and telmisartan group respectively. Although the lowering of blood pressure (both systolic and diastolic) by ramipril or telmisartan...
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