Posted by admin on Feb 25, 2015 in |
A new simple stability indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous estimation of Paracetamol, Tramadol and Dicyclomine in combined pharmaceutical dosage form. An Agilent Zorbax SB C18 (250mmx4.6mm, 5microns) column with mobile phase containing ammonium acetate: acetonitrile (700:300 v/v) was used. The flow rate was maintained at 1.0 mL/min, column temperature was 30°C and effluents were monitored by using a photodiode array detector at 233 nm. The retention times of Paracetamol, Tramadol and Dicyclomine were found to be 2.331, 3.182 and 7.650 min, respectively. Correlation co-efficient for Paracetamol, Tramadol and Dicyclomine were found to be 0.99, 0.99 and 0.99 respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Paracetamol, Tramadol and Dicyclomine in formulations was found to be in a range of 98-102%, 98-102% and 98-102% respectively. Paracetamol, Tramadol and Dicyclomine dosage form is also subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved and...
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Posted by admin on Feb 25, 2015 in |
An Accurate, sensitive, precise and stability indicating high performance thin layer chromatographic method has been developed and validated for the estimation of Glimepiride and Metformin hydrochloride. The method was developed using TLC aluminium plates precoated with silica gel 60F254 as the stationary phase and 0.5% Ammonium Sulfate: Methanol (7.5:2.5 v/v) as mobile phase. Densitometric analysis of Glimepiride and Metformin hydrochloride was carried out in the absorbance mode at 228 nm. The two drugs were satisfactorily resolved with RF values 0.73 and 0.45 for Glimepiride and Metformin hydrochloride respectively. Linearity was found to be 600-2100 ng/band for Glimepiride and 200-700 ng/band for Metformin hydrochloride. The method was found to be accurate, precise, and robust according to acceptance criteria. The limit of detection (LOD) was found to be 0.05 ng/band and 0.32 ng/band for Glimepiride and Metformin hydrochloride respectively. The limit of quantification (LOQ) was found to be 0.16 ng/band and 0.96 ng/band for Glimepiride and Metformin hydrochloride respectively. The proposed HPTLC method was successfully applied for the forced degradation study...
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Posted by admin on Feb 25, 2015 in |
Lannea coromandelica (Anacardiaceae) is a tropical tree commonly called as Indian ash tree. Its various parts is said to possess medicinal property and used in Ayurveda and other ancient system of medicine for the treatment of various ailments including treatment of inflammation, gout, cholera, leprosy, vaginal troubles etc. Its Pharmacological study revealed anti-inflammatory, antimicrobial, hypotensive, anticancerous activities. Leaves of Lannea coromandelica were collected from Azhagar malai, Madurai. The sample specimen of Lannea coromandelica was identified and authenticated by Dr. Jaya Raman, Taxonomist, Chennai. The present work was carried out to identify some of the phytocomponents present in the ethanolic leaf extract of the L. coromandelica by GC-MS technique, to ascertain the medicinal properties of the plant and also to assess its anti-oxidant and anti-arthritic potential of ethanolic leaf extract of Lannea coromandelica. The results revealed that ethanolic extract is mainly composed of oxygenated hydrocarbons and predominantly phenolic hydrocarbons. These different active phytochemicals may be responsible for wide range of activities, which may help in the protection against incurable diseases. The future in vivo investigation...
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Posted by admin on Feb 25, 2015 in |
The antioxidant activity of cultured mycelia of ten species of the oyster mushroom, Pleurotus species namely P. ostreatus, P. flabellatus, P. sapidus, P. citrinopileatus, P.roseus, P.sajor-caju, P.florida, P.cystidouses, P.fossulatus, and P.eryngii was examined. The extracts were screened for their ability to scavenge DPPH, hydroxyl, superoxide radicals and also for ferric reducing power and lipid peroxidation inhibiting activity. Among the ten species studied, all were found to possess DPPH radical scavenging and lipid peroxidation inhibiting properties. P florida scavenged DPPH radicals (94.02%) and P.citrinopileatus inhibited the lipid peroxidation (76.65%) more efficiently than others. Five among the ten Pleurotus species showed ferric reducing power, and SOD activity. P.ostreatus efficiently reduced ferric radicals (51.06%) and scavenged super oxide radicals (74.61%) when compared to others. From the ten species studied all except P.roseus scavenged hydroxyl radicals and the maximum activity was showed by P. sajor-caju (76.17%). The present study thus reveals the profound free radical scavenging activity of cultured mycelia of oyster mushrooms. The findings suggest the potential use of cultured mycelia of...
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Posted by admin on Feb 25, 2015 in |
Oral route of drug administration have wide acceptance up to 50-60% of total dosage forms. Solid dosage forms are popular because of ease of administration, accurate dosage, self-medication, pain avoidance and most importantly the patient compliance. This study was aimed at development of Propranolol hydrochloride and flunarizine dihydrochloride conventional dissolving tablets in combined dosage form. Propranolol competes with sympathomimetic neurotransmitters such as catecholamines for binding at beta (1)- adrenergic receptors in the heart, inhibiting sympathetic stimulation and Flunarizine is a selective calcium entry blocker with calmodulin binding properties and histamine H1 blocking activity both are effective in the treatment of coexistence of hypertension &...
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