Posted by admin on Mar 30, 2015 in |
We identified genes in Fouquieria splendens ssp. breviflora (Fouquieriaceae) that were expressed due to water deficits. RNA was extracted from dehydrated stem samples that were collected directly from Camargo, Querétaro following hydration for two weeks. The plants were grown in two states, drought and hydrated. We then performed subtractive hybridization. The obtained sequences were inserted into a plasmid and sequenced. Analyses were performed using the Blastx and Expasy ScanProsite database. These results revealed the expression of genes that corresponded to the RNA polymerase II β-subunit of chloroplast, rpoC2; the cell binding site known as tripeptide RGD; catalase; and chitinase class I and III. These expressed genes are important because they are involved in the response to oxidative stress and the defense response and development in Fouquieria splendens ssp....
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Posted by admin on Mar 30, 2015 in |
The aim of this study was to determine principle phytochemical classes and total phenolic contents and to investigate antioxidant, anthelmintic and antimicrobial activities of ethanolic extract of Crataeva nurvala leaves. Standard test methodology suitable for each chemical class was followed to screen out major chemical classes preliminarily. The total phenolic content of ethanolic leaf extract was determined using Folin-Ciocalteu colorimetric assay. DPPH free radical scavenging assay and total reducing power test were carried out to investigate the antioxidant capacity. The conventional earthworm method and the Kirby-Bauer disc diffusion test were also performed to assess anthelmintic and antimicrobial activities of the extracts, respectively. Phytochemical screening of the ethanolic leaf extract showed the presence of significant amount of flavonoids and steroids along with other phyto-constituents in minor amount. Folin-Ciocalteu assay showed that 45.53 mg gallic acid equivalent was present per gram of the dried extract of the leaf. Leaf extract exhibited noticeable antioxidant activity in both of the DPPH free radical scavenging assay and the total reducing power test where ascorbic...
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Posted by admin on Mar 30, 2015 in |
An efficient and sensitive RP-HPLC method was developed and validated for the estimation of related substances in Eletriptan hydrobromide drug substance which were identified and characterized by LCMS, FTIR, 1HNMR, 13CNMR techniques. The method was carried out on a Purosphere STAR RP-8e column at temperature 20°C using a phosphate buffer at pH 4.0 and solvent mixture consists of acetonitrile, water and tetrahydrofuran in the ratio of 70:30:1v/v/v in gradient mode of pump. The flow rate is 1.5 ml/min and detection was done at 225nm.The developed RP HPLC method was validated by testing specificity, precision, detection limit, quantification limit, linearity, accuracy, robustness and range. The linearity of the method was confirmed over the range of 0.09 to1.4µg/ml for related substances with correlation coefficients greater than r = 0.999. The accuracy of the method was found to be 98.6 to 102.3% and RSD as found to be less than 2.0% indicating high degree of accuracy and precision for the proposed method. The effective recovery and lower %RSD prove the highness of...
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Posted by admin on Mar 30, 2015 in |
The main objective of present investigation is to formulate the sustained release tablet of lamotrigine using 32 factorial design. Lamotrigine, a weakly basic and anticonvulsant drug belongs to BCS Class II, having high permeability and poor solubility. The sustained release tablets of lamotrigine were prepared employing different concentrations of HPMC K4M and HPMC K100M in different combinations as a rate retarding polymer by wet granulation technique using 32 factorial design. The quantity of polymers, HPMC K4M and HPMC K100M required to achieve the desired drug release was selected as independent variables, X1 and X2 respectively whereas, time required for 10% of drug dissolution (t10%) and 50% (t50%) were selected as dependent variables. Totally nine formulations were designed and are evaluated for hardness, friability, diameter, thickness, % drug content, In-vitro drug release. From the Results it was concluded that all the formulation were found to be within the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept...
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Posted by admin on Mar 30, 2015 in |
Diabetes mellitus (DM) is a complex metabolic disease characterized by chronic hyperglycaemia commonly associated with oxidative stress. The current study aims at assessing the antioxidant and antidiabetic potential of various concentrations of hydroalcoholic extract of Centratherum anthelminticum. The in vitro antioxidant activity was assessed using 1,1-Diphenyl-2-picrilhydrazyl (DPPH), nitric oxide, hydroxyl ion and hydrogen peroxide for free radical scavenging properties of the extracts, and determining their phenol and flavanoid contents. The antidiabetic activity was tested in rats following streptozotocin diabetes induction. The in vitro antioxidant activity using DPPH, nitric oxide radical, hydroxyl radical and H2O2 radical showed IC50 value 68.27 ± 0.34, 82.05 ± 0.46, 113.49 ± 0.86 and 94.71 ± 0.78 μg/ml respectively. The hydroethanol extract of plant seed (250, 500 and 750 mg/Kg) exhibited significant antidiabetic activities with 40.46%, 56.37% and 52.15% glycaemia reduction, respectively. These findings suggest that C. anthelminticum seeds possess both antidiabetic and antioxidant properties, and therefore could be used as starting point for the development of herbal medicines and/or source of new drug molecules...
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