Posted by admin on Feb 25, 2015 in |
The Antimicrobial (antifungal and antibacterial) properties of the different solvents extracts (benzene, pet ether, acetone, chloroform, methanol and aqueous) of Rumex vesicarius Linn plant plants, on four bacterial (Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) and four fungal isolates (Aspergillus niger, Tricoderma reesei, Penicillium funiculosum and Fusarium oxysporum) were investigated by well diffusion assay. The minimum inhibitory concentration (MIC) of these solvents extracts evaluated at different concentration (3.25 mg/ml, 6.25 mg/ml, 12.5 mg/ml, 25 mg/ml and 50 mg/ml) for the tested organisms. The widest spectrum of antimicrobial activity was recorded for pet ether extract of leaf against P. funiculosum (30±1.2mm), F. oxysporum (22±0.8mm), E. coli(20±0.9mm) and P. Aeruginosa (10±0.7mm). This was followed by the benzene extract of leaf, which inhibits P. funiculosume t4l(20±0.8mm), F. oxysporum (18±0.4mm), E.coli(10±0.7mm). Acetone extract of flower work against P. funiculosum (30±1.4mm), A. niger, (12±0.7mm), E. coli(20±0.6mm) and S.aureus (18±0.8mm). The inhibitory effect of chloroform extract of flower against A.niger, (18±0.3mm) and F. oxysporum (16±0.3mm). The antimicrobial activity of methanolic and aqueous extract...
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Posted by admin on Feb 25, 2015 in |
Habenaria plantaginea Lindl. (Orchidaceae) is used as a medicinal plant in several countries. In this study, ethanol, chloroform and water extracts of Habenaria plantaginea were evaluated for antibacterial activity against bacterial (MTCC) strains. Antibacterial activity of this plants extracts were performed using agar disc diffusion method. The antibacterial activity of ethanol extracts showed more effective followed by water and chloroform extracts against all the bacterial strains. Preliminary phytochemical studies were performed for the presence and absence of secondary metabolites in the whole plant. The Minimum Inhibitory concentration (MIC) exhibited by H. plantaginea extract against the bacterial strains ranged between 0.125 mg/ml to 6 mg/ml. The presence of bioactive constituents is associated with the antimicrobial activity of various solvent extracts of this plant was carried in attempt to support the use by medicinal practitioner for the treatment of various diseases. Medicinal importance of this orchid will target to meet the therapeutic...
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Posted by admin on Feb 25, 2015 in |
A new RP-HPLC method was developed and validated for the simultaneous determination of amprolium hydrochloride and sulfaquinoxaline sodium in a powder dosage form. The chromatographic separation was achieved by isocratic mode with Phosphate buffer and Acetonitrile (30:70; v/v), as mobile phase and using C-18 RP-Amide (25.0cm × 4.6 mmi.d, 5µ particle size) as stationary phase, with a flow rate of 1.0 ml/min at ambient temperature. The quantification of the analytes was monitored by UV detection at a wavelength of 263 nm. The optimized method was subjected to validation for specificity, accuracy, precision, linearity, range, ruggedness and robustness. The calibration graphs were linear in the concentration range of 0.08 – 0.12 mg/ml. The results were evaluated as per the International Conference on Harmonization (ICH) guidelines. The precision of the method in terms of RSD is not more than 2% for both the analytes. The method is sensitive, specific, fast and accurate with minimum sample...
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Posted by admin on Feb 25, 2015 in |
Lyophillized floating beads have been investigated as a possible gastro-retentive dosage forms containing Ginger (Zingiber Officinalis) extract ( ZOE) and Nigella Sativa oil (NSO). Emulsion gelation method was used in preparation of ZOE-NSO entrapped floating alginate beads. Drug content and floating properties of the beads were found to be satisfactory. The results revealed that lyophillized beads showed better floating behaviour (> 8 hrs). The formulations containing sodium alginate at 3% w/v concentration with 30 %w/v calcium chloride for cross-linking and 30 min for curing time presented with optimum drug release (8 hrs) and floating time (>24 hrs). The optimized formulation was subjected to storage at 40 and 50º C / relative humidity of 60% for a period of three months. In vitro release data of the optimized batch presented Higuchi-diffusion and Korsmeyer Peppas models with Non-Fickian release. The data proposed that the prepared lyophilized NSO and ZOE hydrogel beads delivered their active ingredients by diffusion and erosion ...
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Posted by admin on Feb 25, 2015 in |
Lansoprazole, a proton pump inhibitor, is used to treat ulcers in the stomach and duodenum. In order to protect this acid labile drug from being degraded in the gastric medium, the marketed oral dosage forms are formulated into delayed release pellets and tablets. The aims of this research were (1) to investigate the principal site of drug release and absorption rate between an over-the-counter (OTC) product and a prescription brand, and (2) to explore the effect of ethanol on the in vitro release profile of the OTC product. For aim No. 1, the in vitro release profile conducted in our lab was convoluted with the lansoprazole plasma concentration time profile of an intravenous bolus reported by Gerloff, et al. For aim No. 2, the dissolution of OTC capsules was studied with 250 mL of 0.1 N HCl combined with equal volume of laboratory simulated beer and wine, which contained 5 and 11.5 % alcohol respectively to compare to its reference, 0% (n=6). Furthermore, the dissolution of the OTC formulation...
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