Posted by admin on Jun 30, 2015 in |
The Human immunodeficiency virus type-1 protease is one of the most important target to highly active anti retrovirus therapy(HAART) for the treatment of all acquired immune deficiency syndrome(AIDS). Protease inhibitor “Darunavir” is most recently included as a PI in the list of HARRT(highly active anti retrovirus therapy), more effective against mutant and wild type simultaneously of Protease with increased no. of H bonding then precursors approved by FDA, So herein we have taken Darunavir as a base structure for virtually identification of more/similar efficient drug like leads then Darunavir using ten different PDB structures (3EM6, 3OXW, 3BVB, 3CYW, 3D1Y, 4DQB, 4DQH, 4DQE, 4DQF, 4DQC & 3EKT) of Protease from PDB database ‘RCSB’ versus chemical compounds database ‘ZINC’ using Schrodinger and Discovery Studio software. Using molecular constraint search with similarity coefficient ‘Tanimoto’, 1,65,000 ligands were extracted and docking analysis were resulted some efficient in docking and in other computational medicinal parameters, we are reporting such lead molecules, and they may further undergo through high end extensive virtual investigation and...
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Posted by admin on May 30, 2015 in |
The present study deals with the evaluation of the antimicrobial activity in methanolic extracts of in vivo (leaf, stem and seed) and in vitro (callus) plant parts of Merremia dissecta and Merremia aegyptia belonging to family Convolvulaceae. The antimicrobial activity against six different microbial strains, four bacterial viz. Bacillus thuringiensis, Bacillus subtilis, Eschrechia coli and Pseudomonas putida and two fungal strains viz. Candida albicans and Aspergillus niger was studied. Initially, plant extracts prepared in four different solvents (Aqueous, Methanol, Ethanol, and Petroleum Ether) were screened for their antimicrobial activity through TTZ test (Tetrazolium Chloride Test). It was assessed that alcoholic sample extracts possessed highest antimicrobial activity amongst all. Later the antimicrobial activity of the methanolic extracts was confirmed using Disc-Agar Diffusion technique. The inhibition zone observed was representative of the antimicrobial potency of that particular sample extract. Zones of inhibition ranging from 6mm to 14mm were observed. The largest zones of inhibition were observed from the leaf extracts of these plants and the leaf extract of Merremia aegyptia also...
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Posted by admin on May 30, 2015 in |
In the present study the anti-arthritic activity of ethanolic extracts of leaf and seed of Elaeocarpus serratus L. was evaluated in vitro using two models namely on the inhibition of protein denaturation and anti-proteinase activities. Indomethacin was used as a standard drug. Results revealed that the ethanolic leaf and seed extracts of E. serratus at two different concentrations (200 and 400 µg/ml) possessed notable activity as compared to the standard drug indomethacin, justifying its usefulness in the treatment of inflammation associated diseases like...
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Posted by admin on May 30, 2015 in |
Diaporthephaseolorum is endophytic fungi isolated from Jatitree (Tectonagrandis L.f). Endophytic microbes were microbes that spent part or all of their life live within plant tissue of a host plant. They produce secondary metabolites with potent anti microbial activity. Jatitree (Tectonagrandis L.f) was one of herbal plants that act as host plants for endophytic microbes that produce secondary metabolites with anti-microbial activity. The current study focussed on isolation of the endophytic microbes from branches of Jati tree and their secondary metabolites. Isolation of endophytes were performed in PDY (potato dextrose yeast) and further extracted using EtoAC. Four fractions (F I – F IV) was obtained from EtOAc extract through column chromatography (n-Hex: EtoAc, ratio 50:1). Further, agar diffusion method was performed to assess their anti-microbial activity. Results demonstrated that F IV has potent towards Staphylococcus aureus (11.10mm). Its GC-MS profile strongly indicated the presence of fatty acid and phenolic related compound. In conclusion, secondary metabolites of endohpytes isolated from Jati tree branches contained predominantly phenol and fatty acid related compound...
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Posted by admin on May 30, 2015 in |
In this present study, the leaf extracts of Citrus hystrix were subjected to a comparative evaluation of preliminary phytochemical screening, membrane stabilizing activity, thrombolytic activity and In-vitro antioxidant activity. The thrombolytic and membrane stabilizing activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK) and standard anti-inflammatory drug, acetyl salicylic acid (ASA), respectively. For thrombolytic activity and membrane stabilizing activity ethanolic leaf extract exhibited clot-lysis value of 13.69% against standard SK (37.43 %) and highest percentage of hemolytic value of 74.40% against standard ASA (93.24%) respectively. DPPH free radical scavenging activity, Cupric Reducing Antioxidant Capacity (CUPRAC), Total Phenolics Content, Total Flavonoids Content, Nitric oxide scavenging assay and total antioxidant capacity were done in the present study. In case of in-vitro antioxidant evaluation all extract forms showed a significant positive results. Phytochemical investigation suggested the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids and...
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