Posted by admin on Apr 30, 2015 in |
Tithonia diversifolia was collected from 27 localities of Côte d’Ivoire. The oils of the leaves and stems obtained by hydrodistillation using Clevenger-type apparatus have been studied by GC and GC-MS. The identified components accounted for 91.4% – 96.9% of leaves and 93% – 95.6% of stems. The content of the main components varied from sample to sample. α-pinene (0.9 – 47.4%), limonene (0.5 – 65.5%), (Z)-β-ocimene (0.0 – 35.9%) and 3-methyl-2 (2-methylbuthenyl) furan (0 – 94%) were noted as major components of the oil from the leaves. Whereas, the stem oil was rich in α-pinene (5.8 – 89.8%), sabinene (0. – 5.2%), β-pinene (0.3 – 10.5%), limonene (1.3 – 36.5%), (Z)-β-ocimene (0 – 39.3%), thymol (0 – 4%) and spathulenol (0.1 – 11.8%). Due to the chemical polymorphisme, the results of oil obtained from leaves and stems were submitted to hierarchical cluster, principal components analysis and discriminant factoriel analysis which allowed the distinction of two groups within the oil samples of each organ. According to the essential oil from...
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Posted by admin on Apr 30, 2015 in |
A simple, rapid, specific, precise and accurate UV Spectrophotometric method for simultaneous estimation of Serratiopeptidase and Diclofenac sodium in bulk and tablet formulation has been developed. UV Spectrophotometric method was developed for the simultaneous estimation of Serratiopeptidase and Diclofenac sodium in tablet formulation. In present work, simple, sensitive, accurate and economical spectroscopic method has been developed for the estimation of Serratiopeptidase and Diclofenac sodium in bulk and its pharmaceutical dosage forms. An absorption maximum was found to be at 275nm for Diclofenac sodium and 264nm for serratipeptidase with the solvent Ethanol 99.99%. Diclofenac sodium follows beer’s law in the range of 5-50μg/ml with correlation coefficient of 0.998 and Serratiopeptidase in the range of 5-50μg/ml with correlation coefficient of 0.995. Results of the analysis were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. The proposed method is simple, rapid and suitable for the routine quality control...
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Posted by admin on Apr 30, 2015 in |
Warfarin and coumarin containing drugs act on Vitamin K epoxide reductase to show its anti-coagulant activity. In this study, three different series of 5- hydroxy coumarin containing phenyl, furan and pyran substituents at third position were designed. Molecular docking studies were performed to determine binding modes and affinities of ligand molecule towards Vitamin K epoxide reductase. In docking studies pair wise linear potential (PLP score) was used as a scoring function and systematic search method to find out bio active conformer. Ligand 2B, 2C, 2D, 2E, 3C, 3D has shown more negative binding energies, which reflects its affinity towards a vitamin K epoxide reductase. Ligand 1A, 1E, 3D has shown maximum hydrogen bond interactions as compared to warfarin. Tyr178, Thr72, Met111, Glu115amino acid residues were involved in the ligand-protein interactions. Hydroxy, methoxy, ethoxy and methyl substitutions has played major roles in the ligand-protein interaction. Therefore, 3-substituted -5-hydroxy coumarins could be served as good drug candidates for anticoagulant activity and additional experimental studies are warranted to locate their importance as...
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Posted by admin on Apr 3, 2015 in |
Honey possesses a wide range of medicinal properties due to presence of phytochemicals including flavonoids and phenolic acids. Free radicals and process of angiogenesis are one of the major contributors to cancer development and the use of phytoconstituents as reactive oxygen species scavengers and as an antiangiogenic moiety has clinical potential and may be having less negative side effects than the existing drugs. This study has explored the phyto-constituents as well as anti-angiogenic and anti-oxidant activity of Indian honey (Kashmir honey and dhanwantari honey) in comparison with manuka honey. The total phenolic content was measured by folin ciocalteau method and antioxidant activity was estimated by DPPH scavenging assay. Anti-angiogenic activity of honey samples were evaluated by chorioallantoic membrane (CAM) assay. Drabkin’s assay was performed in order to check the hemoglobin content of the chorioallantoic membrane sections. Presence of flavonoids and tannins were observed in all honey samples. Higher total phenolic content was found in kashmir honey (6662.67 mg GAE /100 gm honey) than manuka honey (6078.67 mg GAE /100...
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Posted by admin on Mar 30, 2015 in |
In the present study, a new series of 2, 4, 5- trisubstituted imidazoles derivatives were synthesized taking different aldehydes as substitutions. The preliminary characterization of synthesized compounds identified by physical constants determination and TLC. The chemical structures were confirmed by means of IR, 1H-NMR and Mass spectral data. The synthesized compounds were screened for their anti-microbial and antifungal activity using standard methods. Both gram positive and gram negative organisms such as E.coli, B.subtilis, S.aureus, A. niger, C.albicans were used. The compounds screened for their antibacterial and antifungal activities A1, A2, A4 have shown promising antibacterial and antifungal activity against as compared to standard drugs Ciprofloxacin and Griseofulvin. The compounds were also screened for their antidepressant activities using forced swimming test in mice in which A1, A2 and A6 are found to be most significant while compounds A4 and A5 showed moderate activity as compared to standard drug Fluoxetin. The compound A4, 4-(4, 5-diphenyl-1H-imidazole-2-yl) phenol exhibited excellent...
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