Posted by admin on Mar 30, 2015 in |
The main purpose of the study was to examine the effect of QoL of PHN patients after the treatment of tramadol (50mg-200mg), with topical cream containing 3.33% doxepin and 0.05% capsaicin and clinical correlations with CYP2D6 polymorphism. The study was prospective, non-responders versus responders, conducted in 246 outpatients of PHN undergoing tramadol treatment for 4 weeks. Rescue analgesia given in the form of the topical cream consisting of the combination of 3.33% doxepin and 0.05% capsaicin to the affected areas of patients along with tramadol therapy. The QoL as per WHO QoL BREF Questionnaire was evaluated in all the study participants. All samples were analyzed for CYP2D6 (*2,*4 and *10) polymorphism using PCR-RFLP method. Although the QoL score showed magnitude of improvement was higher in responders as compared to non-responders. Insignificant interactions were found in all domains of QoL with respect to CYP2D6*2 and CYP2D6*4 polymorphism. However, a significant (p<0.001) interaction was found in CYP2D6*10 polymorphism with respect to environmental domain of QoL. A significant interaction was found with...
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Posted by admin on Mar 30, 2015 in |
Cervical cancer (CaCx) is the third most common female cancer worldwide with an estimated 5, 27, 624 new cases and 2, 65, 653 deaths in 2014. We conducted a systematic review and meta- analysis to assess safety of prophylactic HPV vaccines compared against placebo. Four unique trials were included out of 607 publications. The total patient data evaluated was 1427(852 in vaccinated arm; 575 in the placebo arm). The pain was the major local side effects with 61% followed by swelling 22% and redness 17% in the vaccinated arm and similarly in the placebo arm pain 72% followed by swelling 18% and redness 10%. Headache was the most frequently experienced systemic side effects 42% in vaccine and placebo group. Unsolicited adverse event in vaccinated and placebo group were 39% and 37% respectively. New onset chronic disease in vaccinated and placebo arm were 3% and 2% respectively. Serious adverse event were 3% in vaccinated and 2% in placebo group. Undesirable pregnancy outcomes were same for both vaccinated and placebo arm...
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Posted by admin on Mar 30, 2015 in |
We have evaluated the efficacy of methanolic extract of Vernonia cinerea (MEVC) in selenite induced cataract using Sprague Dawley rats. Previous reports suggest that phytochemicals or natural plant products retard the process of cataractogenesis by scavenging free oxygen radicals. Hence, the present study sought to assess the potential of MEVC on in vivo selenite induced cataract models. The antioxidant activity was comparatively studied using DPPH radical scavenging and FRAP assay. MEVC exhibited higher DPPH radical scavenging activity as well as reducing power assay. In this study, cataract was induced by a single subcutaneous injection of sodium selenite (4µg/ g body weight) on rat pups. MEVC was administered orally from 8th day upto 21st day at a concentration 5 µg/g body weight. Cataract was visualized on 16th day with the help of an ophthalmoscope and later on with the naked eye. On the 30th day, rats were euthanized by sodium pentothal injection, lenses were excised and the biochemical parameters such as activity of catalase (CAT), Ca2+ ATPase and the content...
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Posted by admin on Mar 30, 2015 in |
Tinidazole is a synthetic antiprotozoal and antibacterial agent which is practically insoluble in water.Oral dose of tinidazole is very high (2gm) owing to its limited solubility. Present research focuses on enhancement of solubility of tinidazole using the technique of solid dispersion which is reported to be one of the simple technique for solubility enhancement using various grades of Gelucire and PEG 6000. The ratio of drug and Carrier was decided based on phase solubility study. Solid dispersions were prepared by different techniques such as physical mixture, kneading, co-precipitation and closed melting method.Compatability between drug and carrier was checked by FTIR studies. Efficiency of solid dispersions was checked by conducting saturation solubility testing in water. Multimedia dissolution was carried in different media’s such as Water, 0.1 N HCl and Phosphate Buffer after checking drug content. The formulations were found to enhanced solubility and dissolution as compared to pure...
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Posted by admin on Mar 30, 2015 in |
An HPTLC method was developed for qualitative and quantitative estimation of catechin, quercetin and β-carotene in the marketed and in house polyherbal formulation Maharishi Amrit Kalash (MAK-5). The method was developed using precoated HPTLC silica gel 60 F254 as stationary phase and toluene: ethyl acetate: formic acid (12:12:2, v/v/v) as mobile phase for catechin and quercetin and petroleum ether (40-600C): acetone (17:3, v/v) for β-carotene. Detection and quantification were performed densitometrically at 200nm for catechin and quercetin and 430 nm for β-carotene. The Rf values of catechin, quercetin and β-carotene are 0.29 ± 0.01, 0.49 ± 0.01 and 0.70 ± 0.01 respectively. The percentage content of catechin, quercetin and β-carotene in marketed and in house polyherbal formulation are 0.223%w/w, 0.099%w/w and 2.313%w/w and 0.114%w/w, 0.141%w/w and 2.486%w/w respectively. Linearity was observed in the concentration range of 100-800 ng/spot for catechin and quercetin and that of 100-600ng/spot for β-carotene. Quantification of catechin, quercetin and β-carotene was found to be achieved by HPTLC fingerprinting from marketed and inhouse polyherbal...
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