Posted by admin on May 30, 2015 in |
Cissampelos pariera, an important medicinal plant was subjected to elicitation and bioconversion of precursors for the formation of Berberine, an important medicinal component. In vitro cultures of Cissampelos pariera, was successfully established using stem and leaf explants. MS Media fortified with IAA (2 mg/l) alone and MS media enriched with IAA (2mg/l) showed profuse callusing from leaf and stem explants respectively. Elicitation with yeast resulted in the highest percentage of berberine yield from leaf callus (0.055%). Elicitation with Bacillus subtilis showed the presence of 0.0294% berberine in leaf callus. Elicitation with Aspergillus niger showed the highest yield of 0.0019% from stem callus. Addition of precursor components Tryptophan and Phenylalanine did not increased the berberine from leaf callus. But addition of precursor components Tryptophan and Phenylalanine showed the bioconversion to 0.0019% and 0.0022% of berberine in stem callus. The study reports a standardized protocol for the efficient conversion of precursors to breberine and also to elicit the production using...
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Posted by admin on May 30, 2015 in |
Objective: To evaluate the antidepressant activity of chronic administration of Tramadol alone and when combined with Fluoxetine in low doses in albino mice. Methods: The antidepressant effect was determined by recording the immobility time in Despair Swim Test. The mice were randomized into seven groups, with six mice in each group. Group 1 mice were given normal saline (10 ml/kg) which served as control. Mice of groups 2, 3 and 4 received Tramadol in graded doses (10, 20 and 40 mg/kg). Fluoxetine (standard drug) was given to mice of groups 5 and 6. Tramadol and Fluoxetine in low doses (10ml/kg each) were co-administered to mice of group 7. All drugs were given intraperitoneally for 7 days and were subjected to despair swim test on the day 7 of drug administration. Results: Tramadol and Fluoxetine have shown significant antidepressant activity when compared to the control. There is dose dependant increase in antidepressant activity of Tramadol. The antidepressant of Tramadol 20 mg/kg was comparable to Fluoxetine 20 mg/kg. Tramadol potentiated the...
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Posted by admin on May 30, 2015 in |
Molecular effects of androgens on cardiovascular system are variable in nature and are concentration dependent. In spite of the evidences favoring possible involvement of reduced endogenous androgens in the pathogenesis of atherosclerotic vascular disease, there is no certain role of exogenous testosterone supplementation in normal or hypogonadal males to slow down the progression of atherosclerosis. Replacement of testosterone in certain trials resulted in significant reduction in the levels of inflammatory mediators which are considered as important biomarkers in atherosclerosis. Present study aimed mainly to configure firstly the onset of inflammatory markers, dyslipidemia, insulin resistance, endothelial dysfunction (cardiovascular risk factors) as developed in castrated male rats. Secondly the role of testosterone replacement using various dose levels, expressed as low, medium and high respectively. Certain Biomarkers were selected for evaluation and are mainly serum glucose, insulin, lipogram pattern, CRP, IL-6, Endothelin-1 and plasma fibrinogen. Castration of rats and almost testosterone depletion has led to disturbance in glucose metabolism, lipogram pattern, increased inflammatory markers and plasma fibrinogen. Testosterone administration induced evident improvement,...
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Posted by admin on May 30, 2015 in |
The present investigation deals with preliminary phytochemical analysis and in vitro antibacterial potentials of different solvent extracts of Gymnema sylvestre were demonstrated. The phytochemical tests of the extracts have revealed the presence of alkaloids, flavonoids, phenols, tannins, terpenoids carbohydrate, tannin, and saponin. Five gram-positive (Bacillus cereus, Bacillus subtilis, Streptococcus cremoris, Streptococcus fecalis, Staphylococcus aureus) and five gram-negative (Klebsiella pneumoniae, Proteus vulgaris, Salmonella paratyphi, Shigella boydi, Shigella dysentriae) bacterial strains were tested. The ethanol extracts have been showed encouraging results. The maximum inhibition were recorded 42.3mm in Bacillus cereus at 250µl concentration followed by E. coli (38.4mm), Streptococcus fecalis (37.2mm), Klebsiella pneumonia (35.6mm), S. aureus (33.3mm), P. aeruginosa (30.5mm), S. cremoris (28.1mm) Proteus vulgaris (26.9mm) B. subtilis (23.5mm) and least inhibition was observed in S. typhi (21.7mm). Moderate activity was observed in chloroform extract. Minimum activity was observed in hexane at different concentration tested. Compared to synthetic antibiotic Ampicillin (50mg), solvent extracts showed significant antibacterial activity. The present findings support to the traditional knowledge of the medicinal plants to the...
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Posted by admin on May 30, 2015 in |
The Turmeric (Curcuma Longa.L) plant, a perennial herb belonging to the ginger family is cultivated extensively in South East Asian countries. The polyphenols Curcumin, De methoxy curcumin and Bisdemethoxy curcumin collectively known as curcuminoids ,which aid in the treatment of degenerative diseases like cancer, alzheimer’s, cardio vascular diseases, arthritis, diabetes and immune deficiencies .Curcuminoids are generally present in the rhizome in the ratio Curcumin : DMC : BDMC 55-60% : 19 -21 % :20–24% respectively. When the rhizhome is extracted and the curcuminoids isolated from the extract,there is a composition change from what is naturally found in the rhizhome. The present study gives a comparative data of bio-absorption of two turmeric formulations containing curcuminoids in a varied composition compared to regular turmeric extract. Regular Turmeric extract with 96.5% curcuminoids and two different formulation containing 7.8% curcuminoids and 51% curcuminoids were orally administered to adult albino rats which were divided into 10 groups based on different doses of test item (50 mg/kg, 150mg/kg, 300mg/kg).The absorption of curcuminoids was studied by...
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