Posted by admin on Jan 31, 2015 in |
Raise in the multidrug resistance gave challenge to the treatment of tuberculosis with first line medication due to less response. Nanoparticle drug delivery has proven efficiency in improving the therapeutic efficacy of many drugs. Reduction of dosing frequency was achieved by applying drug delivery using Nano medicine and Green Nanotechnology. The present study aimed in the production of such drug conjugates of Isoniazid using interdisciplinary technology of nano medicine and green nanotechnology. Isoniazid Nano-conjugates were produced by simple incubation and formed conjugates were analyzed for the efficiency of the plant extract to conjugate the drug. The therapeutic efficacy of the conjugated drug was evaluated using luciferase Reporter phage assay against M.tuberculosis H37RV. High drug entrapment efficiency of plant extract was achieved, ranging from 85-95% and effective particle sized drug-conjugates, ranging from 246-356nm were formed. The relative light units were also less indicating the persistence of activity of the Isoniazid and the inhibition was 93%. Hence Isoniazid Nano-conjugates have the potential for TB Chemotherapy which could be a cost-effective method...
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Posted by admin on Jan 31, 2015 in |
It is estimated that more than 170 million people are suffering from diabetes globally and this number is expected to double by 2030, and the greatest increase in prevalence is, however, expected to occur in Asia and Africa. Diabetes is the most common endocrine disease and its prevalence is reaching epidemic proportion worldwide. Tamarindus indica is a slow growing tree that is resistant to strong winds and perennial. The stem-bark extract of the plant is used locally for the management of diabetes. The saponin-rich portion of the stem-bark extract of Tamarindus indica L. was investigated for its hypoglycaemic action on experimentally induced hyperglycaemic Wistar rats. The oral LD50 of the extract was found to be 1,265 mg/kg. The extract lowered the Blood Glucose Level (BGL) in the three doses used (100, 200 and 400 mg/kg) and was significant at 400 mg/kg dose after the 8th and 16th hours. The 200 mg/kg dose significantly lowered the BGL at 24 hours p < 0.02. The saponin-rich portion of Tamarindus indica Linn...
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Posted by admin on Jan 31, 2015 in |
Cerium oxide is an oxide of the rare earth metal which is widely used for several disorders. However, there are no complete toxicity studies done on it. The purpose of present study is to evaluate acute and Anti-hyper lipidemic activity of cerium oxide nano particles on Wistar albino rats. In this study, Cerium oxide was administered to six rats in different groups in different concentrations (100mg/kg and 200mg/kg) observed for 14 days. After the course, blood was collected and it was calculated for biochemical parameters like serum triglycerides, serum cholesterol, Very low density lipoprotein cholesterol (VLDL), Serum HDL cholesterol, Serum LDL cholesterol levels in the test and standard group of animals which were compared with ccl4 induced group of animals. The results showed that the mechanism of action of anti-hyper lipidemic activity of Cerium oxide nano particles on dose dependent...
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Posted by admin on Jan 31, 2015 in |
This study was carried out with the objective of the antibacterial and antifungal activity of chloroform, ethyl acetate, ethanol, methanol and aqueous extracts of Asafoetida. Antibacterial activity was carried out against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and the antifungal activity was evaluated against Aspergillus niger, Candida albicans. The testing was done by well diffusion method and evaluation was done by detecting zone of inhibition (in mm) and minimum inhibitory concentration (MIC). Studies were perfomed with two different concentrations of all extracts (2 mg/ml and 4 mg/ml). Zone of inhibition were compared with standards like Ciprofloxacin (0.1 mg/ml) and Fluconazole (0.1 mg/ml). The results showed that ethyl acetate, ethanol, and methanol extract has significant antimicrobial activity and highest activity was reported with methanolic extract. The minimum inhibitory concentration of methanolic extract, ethanolic extract and ethyl acetate extract against most of the test microorganisms were 1 mg/ml, 1 mg/ml, 2 mg/ml...
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Posted by admin on Jan 31, 2015 in |
Two methods for simultaneous estimation of Pracetamol and Zaltoprofen in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 245 nm and 227 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 237.5 nm (isobestic point) and at 227 nm the maximum absorption of Zaltoprofen. The linearity ranges for Paracetamol and Zaltoprofen were 2 – 18 μg/ml and 2 – 18 μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 100.02 ± 0.467 and 99.87 ± 0.532 for simultaneous equation method and 99.82 ± 0.483 and 99.84 ± 0.512 for Q analysis (absorption ratio) method for Paracetamol and Zaltoprofen respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control...
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