Posted by admin on Mar 30, 2015 in |
In the present work, we have synthesized a new analoges of monocyclic β-lactam (2-(3-(2, 4-dichlorophenoxy)-2-(substituted aryl) – 4-oxoazetidin – 1 – ylamino) – N – (pyridin-2-yl) acetamide) derivatives in the presence of triethyl amine (TEA) and phosphorus oxychloride (POCl3) under classical method by using Dichloromethane (DCM) as a solvent. The designed compounds 4(a-l) were prepared by Staudinger reaction ([2+2] ketene-imine cycloaddition reactions). In which an azetidin-2-one motif connects with pyridine-2-acetamide nucleus with two aromatic rings. The target compounds were screened for in vitro antibacterial activity against clinically relevant Gram-negative (Escherichia coli and Klebsiella pneumonia) and Gram-positive species (Bacillus subtilis, Proteus vulgaris and Staphylococcus aureus). The obtained results have demonstrated that all the synthesized imidazole-conjugated monocyclic β-lactam derivatives showed good antibacterial activity. Particularly the compounds 4e and 4l found to be effective in P.vulgaris as equal to reference ampicillin and other compounds showed moderate to good activity against five human bacterial pathogens. All these compounds have been characterized by IR, 1H-NMR, 13C-NMR, Mass spectrometry and Elemental...
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Posted by admin on Mar 30, 2015 in |
The methanolic extract of whole plant Coldenia procumbens was screened for its analgesic activity. The crude extract was assessed for central analgesic effect using in-vivo methods like hot plate method, tail-flick method, and tail-immersion method at two different doses. The extract at the dose of 200mg/kg body weight showed significant (p<0.001) analgesic effect comparable to the standard drug Pentazocine at the dose of 10mg/kg body weight. The peripheral analgesic was assessed by acetic acid-induced writhing test in mice. The methanolic extract at a dose of 200 & 400mg/kg body weight found to show significant (p< 0.001) analgesic effect comparable to the standard drug Aspirin at the dose of 20mg/kg body...
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Posted by admin on Mar 30, 2015 in |
The aim of the present study was to isolate, identify and to analyze the phylogenetic characteristics of the potent actinobacterial strain VL-RK_05 isolated from Mango orchards of Vissannapet, Krishna Dist, Andhra Pradesh. Soil samples collected were pre treated with calcium carbonate and used for isolation of potent Actinobacterial strain designated as VL-RK_05. Identification of the strain was carried out by studying the micro morphological, cultural, biochemical and physiological methods. The Phylogenetic study of the strain was carried out by employing 16S r RNA sequence based analysis. Phylogenetic tree was constructed using the Molecular Evolutionary Genetic analysis version 5. Phylogenetic analysis of 16S r RNA sequencing revealed that the strain is closely related to Rhodococcus erythropolis and the bioactive metabolites produced by the strain inhibited Gram positive bacteria (Staphylococcus aureus, Bacillus megaterium), Gram negative bacteria (Shigella flexneri, Xanthomonas campestris, Proteus vulgaris, Pseudomonas aeruginosa and Escherichia coli) and fungi Aspergillus niger, Botrytis cinerea, Fusarium solani F. oxysporum and Candida...
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Posted by admin on Mar 30, 2015 in |
Leaves and bark preparations of Ficus dalhousiae Miq (Moraceae) are used in folklore and Siddha system of medicine for liver and skin ailments. So the intent of the present study was to evaluate the Hepatoprotective effect of Ficus dalhousiae Miq leaves ethanolic extract (FDLEE) by means of Carbon tetrachloride and Paracetamol induced hepatotoxicity model in Albino rats. Administration of carbon tetrachloride suspended in liquid paraffin 1ml/kg body weight i.p, and Paracetamol in distilled water (2 gm/kg) body weight; p.o. to albino Wistar rats resulted in hepatotoxicity. Biochemical parameters [Alkaline Transaminase (ALT/SGPT), Aspartate Transaminase (AST/SGOT), alkaline phosphatase (ALP), Total Bilirubin (TB), Direct Bilirubin (DB), Albumin (ALB), Total Protein (TP)] and liver weight were measured to determine the degree of protection at the end of duration of treatment in both the models. CCl4 and Paracetamol markedly elevated the levels of SGPT, SGOT, ALP, TB and DB. Treatment with the standard drug Silymarin (25mg/kg) reversed all the parameters and restored them to the optimum levels. The test extract FDLEE produced a significant...
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Posted by admin on Mar 30, 2015 in |
The aim of the present study is an attempt to evaluate the antimicrobial activity of the methanolic extract of the ink of cuttlefish, Sepia pharaonis on bacterial pathogens. The methanolic extract of the ink of cuttlefish, Sepia pharaonis were prepared and the antibacterial activity was determined by agar well diffusion method on different bacterial strains (Bacillus sp., Pseudomonas sp., E coli., Staphylococcus sp. and Klebsiella sp.). Isolation and the purification of the antibacterial principle were carried out by using Thin Layer Chromatography (TLC). The thermal stability of the extract was determined by incubating the extract at different temperatures. The methanolic extract of the ink of cuttlefish showed antibacterial activity on different bacterial strains. Of the five different species used, the bacterial species Bacillus were found to be more sensitive than all the other species. The bacterial strains Pseudomonas sp., Staphylococcus sp. and Klebsiella sp. were found to be moderately sensitive to the methanolic extract of the ink of cuttlefish. The species E coli is found to be least sensitive...
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