Posted by admin on Feb 25, 2015 in |
In this investigation, Miconazole Nitrate topical gels were designed by employing various bioadhesive polymers like polyacrylates (carbopol 940), cellulose derivatives (hydroxyl propyl methyl cellulose and sodium carboxy methyl cellulose) and natural polymers (sodium alginate) alone and in combination. The prepared gels were also characterized for different parameters like visual appearance, pH, consistency, rheological studies, drug content, in vitro release studies, stability studies and antifungal activity. From the results it was found that all the formulations were free of gritty particles and were found to be uniform in consistency. The values of pH were within the range of the skin pH that is in between 5.9 and 6.8, which indicates these gels cannot cause any local irritation to the skin surface. The consistencies of the gels were found to be excellent. The viscosity values of the gel increased in the following manner: carbopol and HPMC >carbopol>carbopol and SCMC >carbopol and sodium alginate > Sodium alginate >SCMC: indicating that carbopol in combination with HPMC was found to be the most viscous...
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Posted by admin on Feb 25, 2015 in |
Complexes of Co(II) with macrocylic ligands i.e., 2, 3, 9, 10 tetraphenyl – 1, 4, 8, 11 – tetraazacyclotetradeca – 1, 3, 8, 10 tetraene (BDP), 2, 4, 10, 12 – tetramethyl – 1, 5, 9, 13 tetraazacyclohexadeca 1, 4, 9, 12 tetraene (ADP), 2, 3, 9, 10 tetramethyl 1, 4, 8, 11 tatraazatetredeca – 1, 3, 8, 10 tetraene (DDP) have been synthesized. All these complexes were characterized by elemental analysis, molar conductance magnetic susceptibility measurement, electronic and epr spectral studies. On the basis of elemental analysis the complexes were found to have composition CoLX2. Molar conductance measured in DMF solution indicates that complexes are non-electrolytic in nature except Co(BDP)SO4 which are 1 : 2 electrolytes. Thus the complexes may be formulated as [CoCLX2]. Magnetic moments and the electronic spectra indicate six coordinated distorted octahedral geometry for the complexes. The ligand field parameters were calculated using various energy level diagrams. In vitro synthesized compounds and metal salts have also been tested against same species of plant pathogenic fungi...
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Posted by admin on Feb 25, 2015 in |
Environmental pollution is one of the undesirable side effects of industrialization and found responsible factor for environmental degradation. Water samples and crabs Barytelphusa cunicularis were collected from Gadhinglaj tahsil and analyzed for Physico- chemical parameters and heavy metals respectively. The results showed high range of pH and high values of alkalinity, hardness, chloride, sulphate, TDS, COD. The heavy, metal contamination was in the order of sodium > iron >potassium. The results indicate altered physico-chemical parameters and heavy metals resulting in massive destruction of gill architecture which was concentration and time dependent. Fluctuations in physico-chemical parameters has influenced gill lammelles of crab Barytelphusa cunicularis. Histopathological studies revealed noticeable changes in the gills like clumping of cells, detachment of epithelial surface in primary lamella and destruction of mucosal cells. The gills showed vacuolization in the gill stem, ruptured gill lamellae, connective tissue cells was damaged, destructed with congestion of haemocytes in the gill lamellae are observed. The results obtained were discussed in relation to physico-chemical parameter over respiratory mechanism in crab...
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Posted by admin on Feb 25, 2015 in |
In this pilot study, Zarrouk medium was optimized in terms of biomass production and metabolites for the culture growth of, Spirulina species; Spirulina maxima and Spirulina platensis and their extract were tested against a nosocomial fungal species Candida albicans (C. albicans) to explore anticandidal activity. The corresponding growth and metabolite were measured in terms of turbidity, chlorophyll, carotenoids and protein contents. The culture was harvested by centrifugation and extracted with 75% methanol by freeze thaw method. Supernatant was collected, evaporated to dryness and stored at -20 0C. Anticandidal activities were assessed based on the agar-well diffusion method. The lawn of C. albicans was maintained at 1.5×105 CFU/ml on Sarbourd dextrose (SD) agar plates under sterile conditions. The plates were dried at 37 0C for 30 min. Wells of 6_mm diameter were created by using sterile agar borer. The dried supernatants were dissoluted in normal saline (0.8% NaCl) and poured in each well (100µl); Control well carried 100µl normal saline. Poured wells were incubated for 18 h at 37 0C...
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Posted by admin on Feb 25, 2015 in |
A new simple stability indicating reversed-phase liquid chromatographic method has been developed and validated for simultaneous estimation of Paracetamol, Tramadol and Dicyclomine in combined pharmaceutical dosage form. An Agilent Zorbax SB C18 (250mmx4.6mm, 5microns) column with mobile phase containing ammonium acetate: acetonitrile (700:300 v/v) was used. The flow rate was maintained at 1.0 mL/min, column temperature was 30°C and effluents were monitored by using a photodiode array detector at 233 nm. The retention times of Paracetamol, Tramadol and Dicyclomine were found to be 2.331, 3.182 and 7.650 min, respectively. Correlation co-efficient for Paracetamol, Tramadol and Dicyclomine were found to be 0.99, 0.99 and 0.99 respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. Recovery of Paracetamol, Tramadol and Dicyclomine in formulations was found to be in a range of 98-102%, 98-102% and 98-102% respectively. Paracetamol, Tramadol and Dicyclomine dosage form is also subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. The degradation products were well resolved and...
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