Posted by admin on Mar 31, 2024 in |
Aim and Objective: The main aim of the research work is to formulate and evaluate fast disintegrating tablets of Loratadine by incorporating Ispaghula Mucilage as a Superdisintegrant. Materials and Methods: Tablets were produced utilizing the direct compression technique, incorporating three distinct synthetic superdisintegrants and a natural superdisintegrant (Ispaghula mucilage) at varying concentrations. The primary objective was to reduce disintegration time and enhance the bioavailability of Loratadine. Result: The pre-compression characteristics suggested that the compressibility and flow qualities were favourable. After compression, the tablets exhibited acceptable values for hardness (ranging from 3.4 ± 0.1 to 3.5 ± 0.1 kg/cm2), wetting time (ranging from 15.3 ± 0.5 to 45.6 ± 1.5 seconds), and water absorption ratio, all well within the specified limits. Given that the tablets are meant for oral disintegration, our primary focus was on in-vitro dissolution studies and disintegration time. Among all the formulations, F12 shows shortest disintegration time, merely 8 seconds, and the highest cumulative percent of drug release, reaching 98.67%, to its content of 30mg of Ispaghula...
Read More
Posted by admin on Mar 31, 2024 in |
Tridax procumbens L. is an annual herb that has been used in Ayurveda, Unani, and traditional medicine in India to cure various microbial infections, skin diseases, bronchial disorders, and wound healing. The current study aims to investigate the chemical composition, antioxidant, and antibacterial activities of isolated oil (denoted as TPMG oil) from methanol extract using column chromatography. GC-MS analysis identified a total of six compounds among which 2-pyrrolidinone, 1-methyl (48.68 %), 2, (4H)- benzofuranozone, 5, 6,7, 7a-tetrahydro-4, 4-dimethyl (31.94 %), 2, 2-Dimethoxybutane (8.46%). The antioxidant capacity of TPMG oil with the IC50 value 18.34 μg/mL and significance at P < 0.05 was investigated. The oil was found to be potent bactericidal activity at MIC of 125 µg/mL and 250 µg/mL for S. aureus and P. aeruginosa respectively. Subsequently, the MIC of methanol extract was varied between 125-62.5 µg/mL against selected microorganisms. The ZOI 16.2 ± 0.8366 mm and 15.8 ± 0.8360 mm against P. mirabilis and A.niger were observed. It could be used as an alternative source of antimicrobial...
Read More
Posted by admin on Mar 31, 2024 in |
Microbial biosurfactants are surface-active amphiphilic molecules produced by bacteria, yeast and fungi. Biosurfactant-producing bacteria, often recovered from oil-polluted sites, have significant role in microbial enhanced oil recovery (MEOR), environmental sustainability and pharmaceutical applications. The objective of the present study includes isolation, screening and selection of biosurfactant-producing bacteria from oil polluted sites and characterization of their secreted biosurfactant. To this end, nine bacterial strains were isolated from oil spilled soil in a motor garage and subjected to blood hemolysis test as the preliminary screening for biosurfactant-producing strains wherein six isolates tested positive. Upon subsequent analysis by the oil spreading assay and emulsification index, two potent biosurfactant-producing strains were selected for further characterization. An extensive biochemical, cultural and morphological investigations identified the biosurfactant producing isolates as Bacillus species strain JR3 and Acinetobacter sp. strain JR7. Culture conditions of the two strains were optimized for maximum biosurfactant production. Their secreted biosurfactants were extracted from cell-free culture supernatant using chloroform-methanol precipitation, and characterized by thin layer chromatography which indicated that the biosurfactants were...
Read More
Posted by admin on Mar 31, 2024 in |
Tabernaemontana crassa Benth, synonym Tabernaemontana jollyana Pietre ex Stapf, is a Central African pharmacopoeia plant whose stem bark is widely used in traditional medicine. The aim of this study was to evaluate the acute toxicity of aqueous extracts of this species. Preliminary phytochemical tests carried out on the different crude extracts of T. crassa revealed the following chemical groups: alkaloids, quinones, triterpenes and sterols. Quantitative analysis showed that the hydro-methanolic and methanolic extracts of T. crassa had a high content of total polyphenols and flavonoids (247,33 ± 2,05 mg Eq GA/g Ms and 1543,93 ± 10,15mg Eq Rut/g Ms, respectively). Evaluation of the free radical scavenging capacity of T. crassa extracts (methanolic, hydro-methanolic and aqueous) using the DPPH free radical scavenging method on 96-well microplates showed moderate IC50 values (0,2714 ± 0,0020, 0,2480 ± 0,0006 and 0,3129 ± 0,0005 mg/mL), but still low compared to the reference molecule, ascorbic acid (0,064 ± 0,000 mg/mL). The acute toxicity study of the aqueous extract showed no clinical signs of toxicity at...
Read More
Posted by admin on Mar 31, 2024 in |
Introduction: Clomiphene citrate (CC) is the primary drug of choice for ovulation induction in PCOS but resistance to ovarian stimulation is frequent observation with CC. Letrozole (Let), an Aromatase Inhibitor (AI) induces folliculogenesis by releasing hypothalamo-pituitary axis from tonic inhibitory effect of estrogen. Aim: To compare the rate of folliculogenesis, number of follicle count, ovulation rate, conception rate and pregnancy outcome. Materials & Methods: This prospective comparative study was conducted in RGKMCH & NKIIC, Kolkata from July 2018 to June 2019. In this study 200 anovulatory PCO matched women of 20-40 years were divided into two groups, Group A-100 patients receiving CC 100 mg daily and Group B-100 patients receiving Letrozole 5 mg daily from Day 2 for 5 days. Follicular study started from D9 of cycle and ovulation triggering by using Injection hCG was done as per standard protocol. Patients were evaluated in respect of terminal endocrinological profile, number of follicles, ovulation rate, pregnancy rate & outcome. Results: In Gr A & Gr B, mean no of follicles...
Read More
