Posted by admin on Dec 25, 2014 in |
Two simple, precise, accurate and economical visible spectrophotometric methods were developed for the determination of faropenem in bulk and in tablet dosage forms have been described. The method A was based on the formation of green color complex with Potassium ferri-cyanide in the presence of Ferric chloride, which show maximum absorbance (λ max) at 745nm.The method B is based on the reduction of ferric ion into ferrous ion by the drug in the presence of Ferric ammonium sulphate-1, 10-phenanthroline to form a highly stable Orange red colourferrion complex measured at 511 nm. Mean assays of method A and B was found to be 99.9% and 99.4 % respectively. LOD and LOQ of method A was found to be 2.22 μg/ml and 7.44 μg/ml respectively and for method B it was 7.88 and 26.11 μg/ml.The results of analysis for both the methods have been validated statistically as per ICH guidelines. The proposed methods were simple, sensitive and economical for the quantitative determination of faropenem and were successfully employed for the...
Read More
Posted by admin on Dec 25, 2014 in |
In recent years, synthesis and biological evaluation of novel 4H-1, 4-benzothiazines and their sulfone analogues have gained momentum due to their medicinal and industrial importance. The present article describes the synthesis of new 4H-1, 4-benzothiazines via condensation and oxidative cyclization of substituted 2-aminobenzenethiols with compounds containing active methylene groups. It is believed that the reaction proceeds via intermediary of the enaminoketone system. The sulfone derivatives were synthesized by oxidation of 4H-1, 4-benzothiazines using 30% hydrogen peroxide in glacial acetic acid. The structures of the compounds have been confirmed by spectral and chemical analysis.The synthesized compounds were screened for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS●+) radical cation decolorization assay. The in vitro antibacterial activity was tested against S. aureus, B. subtilis (Gram positive) and E. coli, Pseudomonas aeruginosa (Gram negative) microorganisms using paper disc diffusion method using nutrient agar medium and the antifungal activity of synthesized compounds was tested on fungal strains: A. niger, C. albicans using disc diffusion...
Read More
Posted by admin on Dec 25, 2014 in |
The main aim of the present study was to synthesize new leads with potential antibacterial and antioxidant activities. As a part of systematic investigation of synthesis and biological activity, some of the new thiazoldinedione compounds 5a-e were prepared and screened for their antibacterial and antioxidant activities. The antibacterial evaluation of newly synthesized compounds was carried out by cup-plate method. Antimicrobial activity results revealed that compound 5a showed promising activity against bacteria Staphylococcus aureus, Klebsiella pneumonia, and Pseudomonas aeruginosa, 5e was found to be more active aganist Proteus vulgaris. The antioxidant activity was performed by using free radical scavenging activity by DPPH (1, 1-diphenyl-2-picryl-hydrazil) assay method and Ferric ion reduction method, ascorbic acid was used as reference standard. All the tested derivatives show antioxidant activity but compound 5c and 5d show promising antioxidant activity concentration at 50μg /ml and can be further studied with modifications. Some novel thiazolidinediones derivatives 5(a–e) were synthesized by reacting hydrazine hydrate in ethanol to form hydrazinecarboxylicacid [4-(2, 4-Dioxo-thiazolidin-5-ylidenemethyl)-phenyl-esterand substituted derivatives were formed by further reaction....
Read More
Posted by admin on Dec 25, 2014 in |
The objective of this study was to adopt the analytical method. This adoption procedure was carried out by validating the method parameters and conducting the forced degradation studies to check the interference of the chromatographic response of the degradation products with the drug. In the present study an attempt was made to studies related to system suitability, linearity, accuracy, precision, and assay content, forced degradation, acid and base degradation, hydrolysis degradation and photo stability studies were conducted as per the standard procedures. System suitability conditions meet the recommended criterion. Linearity of the solution was demonstrated between 0.0002577 mg/ml and 0.51540 mg/ml concentration range. Recovery and % RSD are within the recommended limits. Injection reproducibility was demonstrated and the % relative standard deviation for retention time and area were within the limits. Limit of detection value is at 0.00004165mg/ml and limit of quantisation is at 0.00012621mg/ml. Solution was stable up to 38hrs at 5°C temperature. Precision was demonstrated and the percentage relative standard deviation for assay was within the limits....
Read More
Posted by admin on Dec 25, 2014 in |
In an effort to increase yields from a limited area, farmers tend to indiscriminately use more chemical fertilizers and pesticides. The brewery sludge can be directly applied to the soil for the agricultural purposes or it can be composted before utilizing for plant growth. The main objective of the present study is to test the efficacy of the bio-fertilizer in the growth and yield of Chilly Plant. A potted experiment was conducted to evaluate the potential of brewery sludge on different soil chemical properties and growth in chilly plant. The sun dried Brewery sludge was mixed with three different types of soils viz., clay loamy, river sand and red sandy loamy soil in different ratios. Chilly seeds pre incubated in water were sown in earthen pots. There was a significant increase in nitrogen and Potassium concentration in all the three amended soil samples (90%) where as decrease in Phosphate concentration in the amended soils. There was a realistic increase in plant growth in river sand as compared to red...
Read More
