Posted by admin on Apr 30, 2015 in |
Over production of oxygen free radicals, which is mainly mediated by super oxide, occurs in human atherosclerosis. Metoprolol and atorvastatin are the drugs which found to be more prescribed drugs for cardiovascular patients. In the present study, an attempt has been made to evaluate the levels of serum Glutathione, Total anti-oxidant status, lipid peroxidation (malondialdehide) and liver function parameters like serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase. Total 68 subjects were selected for study. Out of 68 subjects 26 were normal healthy volunteers and 42 subjects were patients who are untreated with cardiovascular diseases. The patients both male and female within the age group of 20-70 years were selected. Glutathione, total anti oxidant status, lipid peroxidation, serum glutamate oxaloacetate transaminase and serum glutamate pyruvate transaminase levels in plasma were estimated by using reported methods. Individual methods were standardized first and standard graphs were plotted and the parameters were measured significantly decreased values of glutathione, total anti oxidant status and malondialdehide were observed (p<0.001) in untreated cardiovascular patients as compared with normal...
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Posted by admin on Apr 30, 2015 in |
The antitumor property of the successive, standardized methanolic (MeOH) extract of Aegle marmelos roots (MAM) was investigated in Dalton’s ascites lymphoma mice model. Materials and methods: Flavonoid present in MAM was quantitatively estimated using high performance thin layer chromatography. MAM (100, 300 mg/kg) was administered orally for 15 days to assess antitumor activity. The tumor was induced by DAL cell lines. The mean survival time (MST) and status of the antioxidant enzymes were analysed, 5 fluoro uracil (5FU) was used as standard drug. Results and discussion: Quercetin content in MAM was found to be as 208 mg/gm with the linearity range 200-800 ng. MAM treated tumor bearing mice exhibited significant (p<0.05) increase in the mean survival time. There was a significant improvement in the super oxide dismutase (SOD), catalase levels and (p<0.01) reduction in lipid peroxidation and nitrous oxide (p<0.01) levels were observed with the MAM treatment in comparison to vehicle treated tumor bearing...
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Posted by admin on Apr 30, 2015 in |
Simplify and improve conditions that have been used traditionally to carry out the conventional Biginelli reactioninvolves three-component one-pot condensation of an aldehyde, β-ketoester and urea or thiourea in ethanol under strong acidic condensation HCl for the synthesis of dihydropyrimidinone(DHPMs) and their sulphur analogue dihydropyrimidinthione derivatives which are act as (antibacterial, anti-inflamematory, antiviral, antitumor, antimalarial agents, hypnotics…. ect) by green chemistry approach. These reactions were performed by three-component condensation of different types of an aldehyde (benzaldehyde, acetaldehyde, furfural, cinnamaldehyde, and salicaldehyde), ethyl acetoacetate, and urea or thiourea at reflux temperature under solvent-free conditions without catalyst to afford the corresponding dihydropyrimidinones and dihydropyrimidinthione in excellent yields (76–96%). The advantages of this green chemistry approach the excellent yield, operational simplicity, short time, and the avoidance of the use of organic solvents and friendly preparation. Products were identified using physical and spectroscopic data (IR, 1HNMR, GC. Mass,...
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Posted by admin on Apr 30, 2015 in |
There are some locally available domestic material in our country that can be used in poultry feed. The uses of local material will decrease the feed cost as well as ensure the availability of raw materials. In this investigation we have selected twelve locally available domestic materials that are low cost and nutritious for poultry health. These raw materials play an important role on poultry health. For crude protein estimation we have used manual micro Kjeldahl method. We have applied also micro Kjeldahl automated Foss instrument to measure and compare results of crude protein content of our selected raw materials. Moisture content of these raw materials is also estimated by three different methods, because moisture plays an important role during storing and formulation of poultry feed. In this study it was found that all of the local raw materials contain considerable amount of crude protein. Though all the selected raw materials contain considerable amount of crude protein, among them soya bean meal (SBM) contains higher amount of crude protein...
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Posted by admin on Apr 30, 2015 in |
Enzymes are identified as key catalysts in various processes occurring within the living cells. The role of various enzymes is constantly investigated to find their novel applications. Nitrilase is one such enzyme which carries out the hydrolysis of carbon-nitrogen bonds except for amides. This catalytic property of nitrilase is exploited to generate corresponding chiral carboxylic acids. These chiral molecules can be employed in various drug synthesis schemes. Nitrilase have been reported from various microorganisms with variable catalytic properties. With an objective to explore microorganisms which produce nitrilases and able to catalyze aromatic nitriles, screening of microorganisms from soil was carried out. Out of five soil sampling sites, screening from the soil sample taken from benzonitrile manufacturing industry (Anami Organics Ltd. Valsad, Gujarat-India) resulted in isolation of microorganism which was able to use benzonitrile as substrate and was positive for nitrilase enzyme assay which was done by modified indophenol method. The microorganism was later identified to be Bacillus thuringiensis using 16s rRNA and biochemical analysis. Nitrilase is present as an...
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