Posted by admin on Dec 25, 2014 in |
Members of the genus Ipomoea of family Convolvulaceae besides being some of the most beautiful ornamental plants cultivated for their bell shaped flowers, are also of medicinal value, with some of them reported as cytotoxic agents against some cancer cell lines. The present study evaluates the anticancer activity of the leaf extracts of ten Ipomoea Jacq. Species growing in and around Mumbai, India, on the A 549 cellline and Hep-G2 cell line evaluated by the SRB assay. The crude aqueous extracts of leaves of Ipomoea species studied had significant cytotoxic effect on HEP G2 Cell Line and showed significant inhibitory effect on A549 cell line. , I. Cairica was the best growth inhibitor (GI50at 30.6µg/ml) for HEP G2 cell line followed by I. Hederifolia (GI50 at 38.1 µg/ml), I. Carnea (GI50 at 40.0 µg/ml). Considering the A549 cell line, none of the crude extracts showed GI50 inhibition upto80 µg/ml with the exception of I. aquatic which showed GI50at 75.6...
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Posted by admin on Dec 25, 2014 in |
Objective: Pseudomonas aeruginosa has emerged as an important cause of serious wound infection and death in burn patients. There are a limited numbers of antimicrobial agents affecting the bacterium due to its inherent resistance. Kefir is a natural probiotic which contains diverse spectrum of bacteria, yeasts and their metabolites with potential antimicrobial activity. Current study was designed to investigate the antimicrobial activity of Kefir extract against P. aeruginosa (ATCC 27853) as well as burn clinical isolates. Materials and Methods: In this descriptive study, the Kefir fermented in MRS Broth medium for 24 hours (hrs), 48 hrs and 96 hrs and their extract were used for evaluation of antibacterial effect. The antibacterial effect of different kefir extract were compared based on measurement of diameter of inhibition zone in diffusion plate method, and assessment of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The density of bacteria and percentage of organic acids (lactic and acetic acids) were also tested. Kefir gels were prepared by mixing of equal portions of gel...
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Posted by admin on Dec 25, 2014 in |
The rates of oxidation of uracil and chlorogenic acid (CGA) by tert-butoxyl radicals have been studied by measuring the absorbance of uracil at 258 nm and CGA at 328 nm spectrophotometrically. tert-butoxyl radicals are generated by the photolysis of tert-butyl hydroperoxide in presence of tert-butyl alcohol to scavenge ·OH radicals. The rates and the quantum yields (f) of oxidation of CGA by t-BuO·radicals have been determined in the absence and presence of varying concentrations of uracil. An increase in the concentration of uracil has been found to decrease the rate of oxidation of CGA suggesting that uracil and CGA compete for t-BuO·radicals. Using competitive kinetic studies, the rate constant for the reaction of t-BuO· radical with uracil has been found to be 4.43´ 108 dm3 mol-1 s-1. The quantum yields (fexpt) have been calculated from the experimentally determined rates of oxidation of CGA under different experimental conditions. Assuming that CGA acts as a scavenger of t-BuO·radicals only, the quantum yields (fcal) have been theoretically calculated. fexpt and fcal values...
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Posted by admin on Dec 25, 2014 in |
Madhucalongifolia is an economically important medicinal tree growing throughout the subtropical region. This research article deals with the antioxidant and antimicrobial properties of M. longifolia. The antioxidant properties of the seed were determined by hydrogen peroxide scavenging activity and reducing power assay. The methanol extract exhibited more antioxidant activity than water extract. The fungal and bacterial pathogens used for the study were isolated from the skin scraping of infected animals. The methanol and water extracts of M. longifolia seedswere tested for their antimicrobial efficacy against the pathogens. Methanol extract exhibited good antimicrobial property than water extract. From the above findings it is confirmed that the seeds of M. longifolia posses good antioxidant and antimicrobial...
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Posted by admin on Dec 25, 2014 in |
Objectives: The study aimed to isolate coumarin compound from Bauhinia purpurea bark. Method: The bark was extracted successively with methanol in a soxhlet apparatus. The crude mass was subjected to HPLC, as well as column chromatography and eluted with methylene dichloride – methanol system with increasing methanol ratio to isolate compound BP. The structure of compound BP was identified by various spectrophotometric techniques (UV, IR, 1HNMR, 13CNMR and Mass Spectrometry). Key Findings: Compound BP was obtained as a white crystalline solid with melting point 197oC. It responded positively to the test of flavonoids. It showed λmax at 256 and 356 nm, IR bands at 3258.93, 1343.97, 1655.57, 1165, 1198.52 cm-1, NMR spectra confirmed the presence of phenolic OH and coumarin ring and [ESI-MS] m/z 462.9 [M+– 1]. Conclusion: Concluding from the interpretations of all the spectra, the compound BP was identified as 3, 5, 7- trihydroxy – 2 – (3, 4, 5- trihydroxy phenyl) – 4H-1 – benzopyran – 4- one. This compound had been found worthy for pharmacological...
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