Posted by admin on Feb 26, 2015 in |
In present study to evaluate the mechanisms behind the antioxidant and neuro-protective potential of Orthosiphon staminus methanol bio active guided fraction (OMF) using SH-SY5Y human neuroblastoma cell culture model was carried out. In in-vitro studies, mechanisms of neutralization of H2O2 induced toxicity by OMF using MTT, LDH, ROS, MMP, MDA, Comet assay was performed. Regulation of anti-oxidant enzyme levels by western blot analysis and neuronal biomarker genes (BDNF, TH and AADC genes) at genetic level by quantitative RTPCR was performed. Cells pretreated with OMF effectively prevents the H2O2 induced anti cell proliferation (cell viability), oxidative stress, genotoxicity and significantly enhance (~ 6 fold, p < 0.01) the expression of antioxidant enzymes and increase in (~ 4 fold, p < 0.01) neuronal biomarker gene expression. Present study demonstrated that, significant neuro-protective activity of O. staminus methanol bioactive guided fraction against H2O2 induced oxidative stress by antioxidant mechanisms and enhancement of neuronal biomarker gene expression. This study validate its use by folk medicinal practitioners in India. Therefore, OMF of O. staminus...
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Posted by admin on Feb 25, 2015 in |
Ulcerative colitis is gaining more prominence these days because of its complications (both local and systemic), impairing the quality of life of patient and increased morbidity and mortality rate. For instance, a case was reviewed which proved the role of clinical pharmacist in the treatment plan and in providing a cost effective therapy. The review of the case resulted in a dosage error and prescribing an unnecessary drug that lead to socioeconomic burden on the patient. Hence changes need to be made where a clinical pharmacist plays a key role in providing a rational therapy and better patient...
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Posted by admin on Feb 25, 2015 in |
Cinnarizine was microencapsulated with Eudragit RS100 using an o/o and o/w emulsion solvent evaporation technique. The effects of three formulation variables including the drug: polymer ratio, emulsifier (polyvinyl alcohol) concentration and volume of external medium on the entrapment efficiency and microspheres size distribution were examined. The drug release rate from prepared microspheres and the release kinetics were also studied. The results demonstrated that microspheres with good range of particle size can be prepared, depending on the formulation components. The drug: polymer ratio had a considerable effect on the entrapment efficiency. However, particle size distribution of microspheres was more dependent on the volume polyvinyl alcohol concentration rather than the drug: polymer ratio. The release kinetics was also studied and it was shown that the release profiles of all formulations showed good correlation with the korsmeyer model of...
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Posted by admin on Feb 25, 2015 in |
Depression produces serious emotional and psychological disorders and has severe consequences if not managed at proper time .With the progression of emotional load of depression one is unable to cope up with the extreme negative feelings and tend to create the world of their own thoughts which may end with the end of their life. Whatever forms of symptoms may be related to the grief, depression is far different from normal sadness in that it engulfs our day-to-day life interfering with ability to work, study, eat, sleep, and having fun. World Health Organization W.H.O. (Mental Health and Substance Abuse; Facts & Figures) reported that 15% of depressed persons end their lives in the form of suicide at younger age. Tendency of developing suicidal behavior among the depressed persons is very lethal entangled condition. People who have an impulsive desire to die or perceive suicidal thoughts are very risky. Simultaneously there are many depressed persons who do not have suicidal plan but they prefer to die through some sudden severely...
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Posted by admin on Feb 25, 2015 in |
Fish makes a vital contribution to the survival and health of a significant portion of the world’s population. Upeneus vittatus, a marine species found in the coast of Bay of Bengal, Visakhapatnam was selected for the present study. An attempt is made to know the distribution of Protein, lipids and carbohydrates in muscle and liver of infected and normal Upeneus vittatus. It is observed that a significant difference between infected and normal fish in relation with Lipid content. Lipid levels were observed to be more in the fish with...
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