Posted by admin on Dec 25, 2014 in |
Species of Geum L. genus (avens) of Rosaсeae L. family belong to perspective sources of medicinal plant raw material for obtaining medicines with the anti-microbic and anti-inflammatory action. They are characterized by such an important feature as the presence of biologically active substances, particularly the polyphenol compounds. According to the aim of present work, morphologic and anatomic investigation of leaves, stems, rhisomes with roots of Geum rivale L. of Rosaсeae L. family has been carried out. In order to obtain the data by scientific investigation, the morphologic and anatomic methods of analysis of medicinal plants have been used. In the process of our investigation the diagnostic features of vegetative organs have been determined. The results of morpho-anatomic features can be used in developing analytic and regulatory records intended for incorporation of the additional sources of medicinal herbal raw materials in the practical field. Research of anatomical structure of aboveground and underground parts of G. rivale L with the identification of diagnostic features may be needed during the development of the relevant sections of...
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Posted by admin on Dec 25, 2014 in |
A rapid and sensitive spectrophotometric method is proposed for the determination of Metronidazole and Secnidazole drugs. The method depends on the reduction of Metronidazole and Secnidazole molecules with zinc dust and hydrochloric acid followed by diazotization and coupling with α-naphthylamine to give red colored chromogens easily measured spectrophotometrically at λmax = 510 nm or coupling with 2,5- dihydroxy benzoic acid at λmax = 518nm. The Sandell’s sensitivity, the molar absorptivity, correlation coefficient and the regression equation were calculated. Under optimized experimental conditions Beer’s law is obeyed in the concentration range 3-30 and 2-12 µg/ml for Secnidazole and Metronidazole; respectively with α-naphthyl amine as a coupling agent. Also, by using 2, 5-dihydroxybenzoic acid as a coupling agent, the concentrations obtained were 5-50 µg/ml for both Secnidazole and Metronidazole. Both techniques were applied successfully for the analysis of Metronidazole and Secnidazole in tablets form. These methods are recommended for quality control and routine analysis where time, cost effectiveness and high specificity of analytical techniques are of great...
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Posted by admin on Dec 25, 2014 in |
In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantification of ibuprofen in rat plasma. The mobile phase consisted of a mixture of acetonitrile and water that was adjusted to pH 2.5 using orthophosphoric acid (70:30). The flow rate was set at 0.5 ml/min and effluent was monitored by using UV detector at a wavelength of 223 nm with retention time of 6.1 min. The chromatographic separation was carried out using a C18 (150 mm × 4.6 mm i.d.) column. The proposed method was validated based on linearity, accuracy and precision. The linearity of ibuprofen was in the range of 0.39-50 µg/ml with mean correlation coefficient of 0.999. The percentage mean recovery was found to be at 99.16%, while the coefficients of variation of within-day and between-day measurements were all found to be less than 5%. The limit of quantification (LOQ) and limit of detection (LOD) of the method were 0.296µg/ml and 0.098µg/ml, respectively. The method was further engaged to identify the...
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Posted by admin on Dec 25, 2014 in |
Aim: To investigate the antiparkinsonism activity of hydro alcoholic root extract of P. zeylanica L (PZE) alone and its combination with aqueous extract of C. sinensis leaves (AECS) in reserpine induced model. Materials and methods: Parkinsonism (PD) was induced by sub cutaneous administration of reserpine (1 mg/kg) for 5 days. The extracts/drugs being tested were administered orally (p.o) 60 min prior to the administration of the reserpine for five days. Behavior parameters were analyzed using catalepsy, open field and stepping test. Result: Repeated administration of reserpine (1 mg/kg; s.c) for a period of 5 days significantly increased catalepsy and decreased the locomotor activity as compared to normal animal. All the groups ie L-dopa + carbidopa (syndopa), PZE alone and its combination with C. sinensis showed significant (P<0.001) reduction in catalepsy, akinesia and increase in locomotor performance.Conclusion: It is concluded that P. zeylanica alone and its combination with C. sinensis have a protective effect against PD, while bi-herbal extracts showed more significant protective effect. Hence may offer a safer therapeutic...
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Posted by admin on Dec 25, 2014 in |
Column chromatography of ethyl acetate of Bauhinia retusa afforded a novel flavonoid identified as [ rhamnetin-3-O-α- L-rhamnopyranosyl-3’-O-(prop-1-enyl)], in addition to kaempferol, isorhamnetin-3-α-rhamnoside, qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside, and quercetin 3,7-di-o- β-D-glucoside have been isolated for the first time. Their structures were established from extensive spectroscopic techniques (UV, MS, 1H and 13C-NMR), chemical studies in addition to comparison with literature data and /or authentic samples. More over,qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside and ethyl acetate fraction exerted pronounced antioxidant activities (SC50 µg/ml ,13.5 , 14.7 and 18.7 ) respectively, compared with vitamin C (SC50,,13.9 µg/ml ) by DPPH radical scavenging...
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