Posted by admin on Dec 1, 2014 in |
An attempt was made to study the anti MRSA and antitubercularactivity of Streptomyces sp. BCA1 isolated from Borra Caves soil. In agar plug method, strain BCA1 showed 12-17 mm inhibition against Gram positive and Gram negative bacteria, including MRSA. Crude pigment produced from strain BCA1 by agar surface fermentation showed 15 – 17 mm inhibition in disc diffusion method against bacterial pathogens. In luciferase reporter phage (LRP) assay the crude pigment showed more than 80% reduction against standard strain M. tuberculosis H37Rv, drug sensitive and multi drug resistant (MDR) clinical isolates of M. tuberculosis. Active pigment was separated by TLC and its activity was confirmed by bioassay guided fractionation. The MIC value of the purified pigment against bacterial pathogens ranged between 6.25 and 50µg/ml. MIC value for M. tuberculosis H37Rv and drug sensitive clinical isolate was found to be 6.25 µg/ ml while that of MDR M. tuberculosis isolate was 25 µg/ ml. The solubility of purified pigment in acid and alkali as well as the appearance of absorption...
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Posted by admin on Dec 1, 2014 in |
Twenty two endophytic fungi were isolated from anti-diabetic plants Momordica charantia and Trigonella foenum-graceum. Ethyl acetate extracts of all fungal isolates were tested for inhibition of aldose reductase and carbohydrate metabolizing enzymes. Ethyl acetate extracts of nine endophytic fungi were found to be positive for α- amylase and α- glucosidase inhibitors. Crude extracts of fungal isolates PTFL005 and PTFL006 showed promising inhibition activity on α- amylase with an IC 50 value of 15.48 and 13.48μg/mL respectively. Whereas, the control acarbose had 22.38 μg/mL of IC 50 value for α- amylase at similar experimental condition. Fungal isolates PTFL006 and PTFL011 were found to have potent α- glucosidase inhibitors with an IC 50 value of 17.37 and 10.71μg/mL which was close to the standard acarbose (6.53μg/mL). The only one fungal isolate PMCF003 showed reasonably better inhibition for aldose reductase with an IC50 value of 40μg/mL as compared to standard control quercetin (15μg/mL). All the active endophytic fungal isolates were identified by DNA sequencing method. The most active isolates identified asTrichoderma atroviride...
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Posted by admin on Dec 1, 2014 in |
The objective of the present study is to develop a rapid HPTLC method for the identification, separation and quantification of some amino acidsfrom two species of Marchantia. Three different mobile phases were used for the separation and estimation of eight amino acids (alanine, glycine, methionine, threonine, valine, leucine, histidine and isoleucine). TheHPTLC analysis of Marchantia palmata and M. nepalensis, showed the presence of all the eight amino acids. The content of all the amino acids in Marchantia nepalensis were higher than M. palmata. Of all the amino acids, the glycine content (0.417 mg/g dw in Marchantia nepalensis and 0.287 mg/g dw in M. palmata) was found to be maximum and valine content (0.053 mg/g dw in Marchantia nepalensis and 0.031 mg/g dw in M. palmata) was found minimum. The presence and content of amino acids in the liverworts has been for the first time evaluated by a modern technique like HPTLC. This method provides an important tool for the quantitative evaluation of important amino acidsandgives more accurate results than...
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Posted by admin on Dec 1, 2014 in |
The synthesis of nanoparticles has become the matter of great interest in recent times due to its various advantageous properties and applications in various fields. Though physical and chemical methods are more popular for nanoparticle synthesis, the biogenic production is a better option due to eco-friendliness. Morinda coreia leaf ethanol extracts was screened for the synthesis of Silver nanoparticle using 0.1mM concentration of silver nitrate. The peak of 482 nm in UV-Vis Spectroscopy confirmed the presence of silver nanoparticle synthesis. Biosynthesized silver nanoparticles characterized by Scanning Electron Microscope (SEM), Fourier Transform Infra Red Spectroscopy (FT-IR) and Energy-dispersive X-ray spectroscopy (EDX). The silver nanoparticles around 90 nm were formed. Biologically synthesized silver nanoparticles were further examined for antimicrobial activity against both Gram-negative and Gram-positive bacteria. It was observed that a clear zone of growth inhibition was against in E. coli, Vibrio cholerae, Staphylococcus aureus, Bacillus sps, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serratia marcescens, Proteus vulgaris, Staphylococcus mutans and Salmonella typhi confirms the antibacterial property of biologically synthesized...
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Posted by admin on Dec 1, 2014 in |
Metallothionein (MT) is a cysteine-rich protein that binds to heavy metals, and it is induced by them. The objective of this study to identify the MT protein induction/expression and localization in liver and kidney tissues (treated with 20 mg/L of CdCl2 for 72 hours) by immunohistochemical techniques and Western Blotting methods in the fresh water catfish, Clarias gariepinus. MT expression (MT-I and MT-II) levels were analyzed by Western blot method (using Mouse Monoclonal Anti-Metallothionein primary and Horseradish Peroxidase (HRP) secondary antibody). MT bands were detected using enhanced chemiluminescence system (West Femto Super Signal detection kit, Thermo Scientific Inc. USA) method. Antibodies showed a positive cross reactivity with MT proteins, MT immune activity was high in the cytoplasm of hepatocytes in Cd exposed fish liver and also detected in the nephrocytes present in the proximal tubules of the nephron and more precisely in their basal labyrinth in kidney. Western blotting showed increased MT expression levels in Cd treated tissues when compared with control tissue. The differential induction/localization of MTs in...
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