Posted by admin on Dec 25, 2014 in |
In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method has been developed for the quantification of ibuprofen in rat plasma. The mobile phase consisted of a mixture of acetonitrile and water that was adjusted to pH 2.5 using orthophosphoric acid (70:30). The flow rate was set at 0.5 ml/min and effluent was monitored by using UV detector at a wavelength of 223 nm with retention time of 6.1 min. The chromatographic separation was carried out using a C18 (150 mm × 4.6 mm i.d.) column. The proposed method was validated based on linearity, accuracy and precision. The linearity of ibuprofen was in the range of 0.39-50 µg/ml with mean correlation coefficient of 0.999. The percentage mean recovery was found to be at 99.16%, while the coefficients of variation of within-day and between-day measurements were all found to be less than 5%. The limit of quantification (LOQ) and limit of detection (LOD) of the method were 0.296µg/ml and 0.098µg/ml, respectively. The method was further engaged to identify the...
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Posted by admin on Dec 25, 2014 in |
Aim: To investigate the antiparkinsonism activity of hydro alcoholic root extract of P. zeylanica L (PZE) alone and its combination with aqueous extract of C. sinensis leaves (AECS) in reserpine induced model. Materials and methods: Parkinsonism (PD) was induced by sub cutaneous administration of reserpine (1 mg/kg) for 5 days. The extracts/drugs being tested were administered orally (p.o) 60 min prior to the administration of the reserpine for five days. Behavior parameters were analyzed using catalepsy, open field and stepping test. Result: Repeated administration of reserpine (1 mg/kg; s.c) for a period of 5 days significantly increased catalepsy and decreased the locomotor activity as compared to normal animal. All the groups ie L-dopa + carbidopa (syndopa), PZE alone and its combination with C. sinensis showed significant (P<0.001) reduction in catalepsy, akinesia and increase in locomotor performance.Conclusion: It is concluded that P. zeylanica alone and its combination with C. sinensis have a protective effect against PD, while bi-herbal extracts showed more significant protective effect. Hence may offer a safer therapeutic...
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Posted by admin on Dec 25, 2014 in |
Column chromatography of ethyl acetate of Bauhinia retusa afforded a novel flavonoid identified as [ rhamnetin-3-O-α- L-rhamnopyranosyl-3’-O-(prop-1-enyl)], in addition to kaempferol, isorhamnetin-3-α-rhamnoside, qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside, and quercetin 3,7-di-o- β-D-glucoside have been isolated for the first time. Their structures were established from extensive spectroscopic techniques (UV, MS, 1H and 13C-NMR), chemical studies in addition to comparison with literature data and /or authentic samples. More over,qurcitrin, quercetin 3-O-β-D-glucopyranosyl-β-D-glucopyranoside and ethyl acetate fraction exerted pronounced antioxidant activities (SC50 µg/ml ,13.5 , 14.7 and 18.7 ) respectively, compared with vitamin C (SC50,,13.9 µg/ml ) by DPPH radical scavenging...
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Posted by admin on Dec 25, 2014 in |
Natural medicines and nutraceuticals are good sources of anti-oxidant and are gaining popularity for their capacity to enhance, health-protective effects. The aim of the present study was to investigate the anti-oxidant activity of some natural medicine from the Himalayan region of Nepal. Carefully collected plant materials were subjected to maceration with methanol for about 48 hours. Anti-oxidant activity of methanolic extracts was assessed using DPPH as a free radical in different concentrations (0. 1 µg/ml, 1 µg/ml, 10µg/ml, 100µg/ml). Percent inhibition and effective Concentration (EC50) values were calculated. EC50 of Acacia catechu (0.89µg/ml), Cissus repens (3.44µg/ml) Psidium guajava (3.65µg/ml) were calculated and found to be more effective than ascorbic acid (4.48µg/ml). Drynaria propinque (5.21µg/ml) Eskemukerjea megacarpum (5.24µg/ml) and Nelumbo nucifera (6.76µg/ml) were comparable to standard anti-oxidants whereas Ficus relifiosa, Tinospora sinensis, Elaeocarpus sphaericus, and Cannavis sativa showed poor free radical-scavenging activity. Our results revealed that Acacia catechu (resin) showed greater anti-oxidant activity than other extracts and standard, suggesting that it could be the better anti-oxidant and diseases preventive remedies...
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Posted by admin on Dec 25, 2014 in |
Mycobacterium tuberculosis is the causal agent of tuberculosis which is an infectious disease responsible for more than 2 million deaths per annum worldwide, mainly in developing countries. Cell division of this microbe is very crucial for propagation. Filamenting temperature sensitive mutant Z (FtsZ) is very important player that seems to assemble into a dynamic ring (Z- ring) on the inner surface of the cytoplasmic membrane which involved in stabilizing Z-rings at the place where the division occurs and the formation of the ring is the signal for septation to begin. To control this disease researchers have investigated many naturally occurring bioactive molecules from different medicinal plants. In the present docking study, we screened a total of fifty-one different bioactive molecules found in three medicinal plants namely Justicia adhatoda, Abrus precatoriu sand Dracaena angustifolia to determine the inhibition against M. tuberculosis FtsZ cell divisional protein. No inhibitory effect was observed for the molecule found in D. angustifolia on the other hand, three compounds namely abrectorin, precatorine and gallic acid of...
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