Posted by admin on Feb 29, 2024 in |
Background: Bauhinia purpurea L. is a flowering plant belongs to family Leguminosae, also known as purple orchid plant. All parts of this plant including leaves, stem bark, flower and pods are edible and used for various medicinal purposes. Detailed pharmacognostic profiling of various parts has been performed except plant Pod, thus pod was selected for this study. Objective: The purpose of this work was to enrich the research on B. purpurea L. Pod in respect of pharmacognostical, physicochemical, qualitative identification of secondary metabolites by HPTLC and ethno-botanical profiling. Material and Methods: Entire pod and powder were subject to macro and microscopic study, different physico-chemical parameters including ash, extractive, moisture and pH determined through standard methods with analytical grade chemical and standard equipments. Quantitative estimation of phenolic content was performed by well-known Folin-Ciocalteu method and HPTLC developed through Vision cat software. Result: Fresh pod is green, elongated with sub-orbicular seeds. TS show different parenchymatous arrangement with yellow brown tannin content and Ca-oxalate crystals. Alkaloids, tannin, phenolic and flavonoids were reported...
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Posted by admin on Feb 29, 2024 in |
The quantitative measurement of Axitinib and Avelumab has been created using a simple, quick, precise, sensitive, and reproducible reverse-phasehigh-performance liquid chromatography (RP-HPLC) method. It is more difficult to analyse varying amounts of pharmaceutical active medicinal ingredients in dose forms without any interferences. Therefore, the objective of the current work is to estimate Axitinib and Avelumab simultaneously by adopting an Analytical Quality by Design (AQbD) arotatable central composite-based technique using RP-HPLC-based method development and validation. Axitinib and Avelumab were separated by chromatography using a Hyperclone 5µ BDS C18 130A (150 x 4.6 mm, 5μ) column and a mobile phase made up of Acetonitrile: 0.1% TEA pH-2.5/OPA in a ratio of 45:55 v/v. The flow rate was 1.2 ml/min, and a Photodiode Array Detector operating at room temperature was used to detect absorption at 219 nm. ICH criteria have been used to validate the offered techniques’ linearity, accuracy, precision, and other attributes. The degradation study’s findings showed that the medications deteriorated in high-stress situations. The chemical and pharmaceutical sectors might easily...
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Posted by admin on Feb 29, 2024 in |
Medication Adherence is defined as “the extent to which a person’s behavior in taking medication, lifestyle management, diet, corresponding with an agreed recommendation from a healthcare provider”. Hypertension also called high blood pressure is defined as persistency with an elevation in systolic blood pressure ≥140 mmHg and /or diastolic pressure ≥ 90 mmHg. A cross-sectional study was carried out in order to measure medication adherence, Morisky Green Levine-Medication Adherence Questionnaire developed by Donald Morisky was used to assess medication adherence assessment, which consists of 4 questions (items) whose wording questions are formulated to avoid answering ‘yes’ or ‘no’ and three levels of medication adherence: high, medium, and low were measured based on the scores 0, 1-2,3-4 respectively. The study carried out an evaluation of medication adherence among hypertensive patients. Among 245 respondents, 5.7% were found to be highly adherent, 37.6% were found to be medium adherent and 56.7% were found to be low adherent. A significant association between a family history of hypertension, diabetes, and smoking behavior was observed....
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Posted by admin on Feb 29, 2024 in |
C. splendens is a medicinal plant used for various ailments within the West-African Community. This study sought to investigate the pharmacognostic characteristics, mycobactericidal, and mutagenic activities of the hydro-ethanolic leaves extract C. splendens. The organoleptic, microscopic, macroscopic and physicochemical characteristics were investigated according to WHO, 2011 quality control methods for herbal materials. The mycobactericidal and mutagenic activities were investigated by the agar well diffusion assay and the Ames test (Muta-ChromoPlateTM) respectively. Present in C. splendens are tannins, flavonoids, glycosides, saponins, steroids and alkaloids which are comparable to published literature. The leaves of had anomocytic stomata, and starch granules, while calcium oxalate crystals were visibly absent. The 50% ethanol extract of C. splendens was not active against M. smegmatis at the highest concentration of 100 mg/mL. C. splendens was also non-mutagenic. Though the extract exhibited no mycobactericidal activity at the highest tested concentration, the documented pharmacognostic characteristics can be used for the purposes of authentication. Also, the non-mutagenic potential indicates a lack of toxicity in that...
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Posted by admin on Feb 29, 2024 in |
Aspartame is the most common artificial sweetener which is known to induce oxidative stress. Our goal was to determine whether Boeravinone B and Chebulinic acid protect HepG2 cells against Asp-induced oxidative stress in HepG2 on a multifarious level. In this investigation we have addressed ameliorative role of BB and CA in due course of Asp exposure by evaluating biochemical and molecular parameters. We found that Aspartame induced oxidative stress in HepG2 cells through elevation of ROS and RNS which was further attenuated by BB and CA. Associated levels of SOD, CAT, GPx, Protein carbonyls, Lipid peroxides and Caspase 3/7 in Asp treated cells were found to be risen but BB and CA supplementation restored these back to normal. It was revealed from fluorescence microscopy as well as flow cytometric analysis that BB and CA reversed the proportion of apoptotic, necrotic as well as autophagic cells which was found to be triggered during Asp exposure. mRNA expression of marker genes associated with Apoptosis, Necrosis and Autophagy was observed to be...
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