Posted by admin on Dec 25, 2014 in |
A simple, rapid, RP-HPLC method developed for the simultaneous estimation of paracetamol and zaltaprofen in commercial pharmaceutical formulation by applying Derringer’s desirability function. The dependent variables such as Capacity factor of first peak, α value and retention time of tR3 as the responses with three important independent factors of organic phase composition, pH of mobile phase and flow rate, were used to design mathematical models. The experimental responses were fitted into a second order polynomial and the three responses were simultaneously optimized. The optimum assay conditions were: Methanol-Water (pH 3.5 adjusted with 10 % O-phosphoric acid) (60:40 % v/v)as the mobile phase and at a flow rate of 0.98 ml/min. while using this optimum condition, baseline separation with a minimum resolution of 2.0 and a run time of less than 10 min were achieved. This method showed good agreement between the experimental data and predictive value throughout the studied parameter space. The optimized assay condition was validated according to ICH guidelines to confirm specificity, linearity, accuracy and...
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Posted by admin on Dec 25, 2014 in |
Phytoestrogens are the xenoestrogens which are derived from the plant. This is the first report providing the deeper inside into the mechanism involved in restricting breast cancer progression with the help of docking simulation by phytoestrogen. Docking experiment bring to light that phytoestrogens prevents the binding of oestradiol with its receptor, thereby down regulating the signalling pathway and also acts as inhibitor of enzymes involved in the biosynthesis of endogenous oestradiol. In the present study, thirty phytoestrogen reported from A. racemosus were selected as ligand along with reference compounds using Maestro 9.3. Rutin, shatavarin I, 3, 6, 4′ – trimethoxy-7-O-β-D-glucopyranosyl [1→4]-O-α-D-xylopyranoside glucopyranpsyl, 8-methoxy-5,6,4-trihydroxyisoflavone-7-O-β-D-glucopyranoside,shatavarin X, racemoside A, immunoside showed better interactions with their targets indicated from their respective dock...
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Posted by admin on Dec 23, 2014 in |
In the present work , novel 2-Amino-N’-(2-oxoindolin-3-ylidene) benzo[d]oxazole-5-carbohydrazides were synthesized and evaluated for In-vivo anti-inflammatory activity by carragenan induced rat paw edema method and were known to exhibit promising anti-inflammatory activity at a dose of 30mg/kg bw. Compounds D2, D3, D5 and D8 were found to be comparatively potent with the standard...
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Posted by admin on Dec 1, 2014 in |
Rheumatoid arthritis is a major ailment among autoimmune disorders. A large number of herbal extracts were used for the treatment of various types of rheumatoid disorder. The leaf and root of Justicia gendarussa belonging to Acanthaceae family, commonly known as Nilinirgundi is traditionally used for chronic rheumatism. The present study deals with in-vitro anti-arthritic activity in pharmacological models were studied such as inhibition of protein denaturation and trypsin (proteinase) inhibitory activity. Different fractions and isolated compound from methanolic extract of leaf and root of J.gendarussa with different concentrations (10,100,1000µg/ml ) were studied and results were compared with standard drug Indomethacin. The methanolic extract of leaf and root of J. gendarussa showed dose dependent activity which was found comparable to that of standard drug Indomethacin. Isolated compound-La of leaf of J. gendarussa inhibited the activity of trypsin with IC50 values 34.85 µg/ml as compared to standard drug Indomethacin with IC50 value 11.57 µg/ml. Compound-La of leaf of J. gendarussa showed protein denaturation inhibition at the IC50 value of 24.74 µg/ml...
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Posted by admin on Dec 1, 2014 in |
This work involves synthesis of pyrazolyl thiazine derivatives from different chalcones [1(a-b)] synthesized from substituted acetophenones and different aromatic aldehydes in basic medium by Claisen-Schmidt reaction. These chalcones [1(a-b)] on condensation with different hydrazides [2(a-b)] and isothiocyanates [3(a-b)] when irradiated with microwaves (20%, 140watts) in scientific microwave oven, give different 2-phenyl-2,5-dihydro-pyrazole-1-carbothioic acid phenyl amide derivatives [4(a-g)] which on further MW assisted condensation with substituted acetophenones and different aromatic aldehydes, furnish pyrazolyl thiazine derivatives [7(a-j)]. Library of such pyrazolyl thiazine derivatives has been generated and the structures were subjected to PASS for their probabilities of being active biologically. QSAR study of the library was done to find out most active...
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