Posted by admin on Nov 1, 2014 in |
The increasing resistance of microbes to conventional drugs has promoted the scientists to search for new biocides with broad activity. Plant derived essential oils (EOs) have been known to show antimicrobial activity against specific pathogens and therefore could be considered as alternative antimicrobial agents in controlling pathogens. The present study was done to investigate the antimicrobial activity of soybean, rice, mustard, olive, coconut and castor oil against 24 isolates of 6 different types of food borne pathogens. Antibacterial activities of essential oils were determined using agar disc diffusion assay. All EOs showed antibacterial activity against Escherichia coli, Pseudomonas sp., Staphylococcus aureus, Vibrio cholerae, Klebsiella spp., and Salmonella spp. Staphylococcus aureus was found more sensitive to soybean and mustard oils compared to other Gram negative bacteria. Castor oil was effective against most of the bacteria namely Escherichia coli, Staphylococcus aureus, Vibrio cholerae, Klebsiella spp. and Salmonella spp. The antibacterial activity of EOs could be used for treatment of bacterial infection and preservation of...
Read More
Posted by admin on Nov 1, 2014 in |
Halfmer of chicoric acid derivatives in two series were planned to synthesized and evaluated for their capacity to reduce the viral load in the human plasma by ROTOR GENE-Q (5 Plex) Real Time PCR method to check their biological activity. Different substituted hydroxy cinnamic acids has been conjugated with different amino acid in N-series and in K-Series same substituted hydroxy cinnamic acids has been conjugated with thio derivative of same amino acids In Anti-HIV evaluation K series (Containing Sulfur as a linker) was found to be have highest activity in terms of in-vitro viral load. Compound K-11 (2-{[(2E)-3-(4-hydroxyphenyl) prop-2-enoyl]-sulfanyl}propanoic acid) was found to be most active with viral load 72 copies/ml and 91.69% decrease in viral load. Among two series K (Mercapto series) is more active and among all compounds K-11 is most active compounds against HIV-1 virus in terms of viral load. So, the activities of compounds constitute a strong rationale for further...
Read More
Posted by admin on Oct 31, 2014 in |
A new simple and rapid reverse-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous estimation of cefpodoxime proxetil in bulk drugs and pharmaceutical dosage form. The separation was accomplished on a sunfire C-18 (250 × 4.6 mm, 5 μm) column with a mobile phase consisting of Methanol; Water (70:30 v/v) at a flow rate of 0.8 mL/min. The eluents were monitored at 236 nm by using PDA detector. The retention time for cefpodoxime proxetil was found to be 4.5 min, respectively. The cefpodoxime proxetil followed linearity in the concentration range of 5-30 μg/mL (r2 = 0.998). The amount of drug estimated by proposed method was found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision. The LOD and LOQ values were found to be 0.0004 and 0.0012 μg respectively for cefpodoxime proxetil. The proposed method can be used for routine analysis of both these drugs simultaneously in their combined dosage...
Read More
Posted by admin on Oct 1, 2014 in |
Flavonoid isolated from Mimosa pudica was evaluated for its radical scavenging and immunomodulatory activities in-vitro. Aim of this study is to isolate flavonoid from Mimosa pudica and to determine its antioxidant and immunomodulatory properties. Flavonoid was isolated from dried samples of Mimosa pudica using soxhlet extraction in 80% methanol and purified by column chromatography. Various tests were done for the presence of flavonoid, and the isolate was confirmed as flavonoid by Thin Layer Chromatography (TLC) and Fourier Transform Infrared (FT-IR) analysis. Antioxidant activity of the isolated flavonoid was evaluated using assays such as the 1, 1‑diphenyl‑2‑picryl‑hydrazil (DPPH) radical scavenging assay and hydroxyl radical scavenging assay. In-vitro assay of immunomodulatory activity of flavonoid isolated from Mimosa pudica was done by using lymphocytes isolated from a human blood sample. The isolated flavonoid exhibited a dose-dependent enhancement in activity in DPPH assay and hydroxyl radical scavenging assays. In DPPH assay 10μg of ascorbic acid, equivalent activity was shown by 30μg of isolated flavonoid. The isolate exhibited strong DPPH radical scavenging activity with...
Read More
Posted by admin on Oct 1, 2014 in |
Onychomycosis representing about 30% of mycotic cutaneous infections is the fungal infection that affects the nails. Although not life-threatening, it adversely affects the psychology of the patient whose nails become yellow, discolored, brittle, and unsightly. It may also pose serious problems in immunocompromised individuals. The current market of products for the treatment of onychomycosis includes topical antifungal gels, creams, medicated nail lacquers, and oral antifungals in the form of tablets. Other techniques like nail etching, use of microneedles & mesoscissioning are being studied. The authors propose the formulation and evaluation of glycerogelatin for an effective transungual delivery. Glycerogelatin are semisolid dosage forms comprising of glycerine, gelatin, water and drug which upon heating to temperatures slightly above body temperature melt and can be applied over the affected area. These upon cooling harden, cover the area and release the drug. The authors propose the use of HPMC for an effective glycerogetin formulation. This research article is an attempt to bring into the limelight the potential of glycerogelatins for transungual drug...
Read More
