Posted by admin on Nov 30, 2023 in |
Antibiotic resistance is one of the gravest threats to human health today. Antibiotic resistance has emerged among hazardous bacteria due to the widespread use of drugs for human health, marine life, and agricultural animals. In contrast, bacteriophages are viruses that parasitize other bacteria for sustenance. A host cell is necessary for phage development and may be found practically everywhere in this ecosystem. The emphasis of the research is on the therapeutic application of bacteriophages in the treatment of a Multidrug resistant potential bacterial pathogens isolated from hospital waste and identified as Staphylococcus saprophyticus ATCC 15305, Staphylococcus aureus, Escherichia coli, Pseudomonas sp. populations, as well as determining their MIC90 value against four different antibiotics, are controlled with the use of bacteriophages recovered from waste as well as river water. In addition, the isolated bacteriophages are coated and packed into prepared sodium alginate microsomes. Furthermore, the stability of the microspheres is analyzed artificially under different simulated gastrointestinal conditions (phage titers of 4.7 Log10 PFU/ml to 5.04 Log10 PFU/ml were obtained after...
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Posted by admin on Nov 30, 2023 in |
Consistently the preparation of transition metal complexes of β-diketones has been (and it still is) an area of the highest interest for inorganic and organic chemists, as can be inferred considering the quantity and diversity of research developed in this field. 1,3- Diketones are wide range of valuable molecules including several types of carbocycles and heterocycles hence have high interest. In fact, excessive amount of therapeutical uses are associated with these 1,3- Diketone compounds. The present work is based mainly on the ultrasound irradiation synthesis of various β-diketone ligands and their Fe (III) complexes. At ambient temperature, the clinically active and functionalized various β-diketones has been synthesized from Baker–Venkataraman transformation and its Fe (III) complexes has been prepared and characterized by physical, spectral and analytical data. The different spectroscopic analysis like Mass, 1H-NMR, 13C-NMR, IR, UV and elemental analysis were done. The functionalized beta-diketones showed a certain behaviour and behaved as bidentate ligand and co-ordinate with the transition metal atom through beta-diketo system. The complexes have general formula [ML2]....
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Posted by admin on Nov 30, 2023 in |
Valsartan is a selective angiotensin type I receptor agonist that has been used in the treatment of hypertension disease. The development of a thermodynamically stable and infinitely dilutable nanoemulsion (O/W) of valsartan with surfactant at a low concentration that can enhance its solubility and stability came about as a result of our investigation into the effectiveness of nanoemulsion as a carrier vehicle for Valsartan delivery in this study. Using the water titration methods, by adjusting the ratio of oil, surfactant, and co-surfactant for carrying out the nanoemulsion region, a pseudo-ternary phase diagram was constructed. For each phase, multiple concentrations of oil and surfactant were combined in variable amounts. After gradual titration with water phase utilizing a vortex mixer for each ratio of oil, surfactant, and co-surfactant (Smix), the translucent and transparent nano-emulsions were discovered. The optimization of surfactant and cosurfactant was done using a pseudo ternary phase diagram which were showing large covered area. According to the study, particle size, polydispersity index, zeta potential, physical stability, and shape were...
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Posted by admin on Nov 30, 2023 in |
This article discusses the assessment of kidney function in patients with coronavirus-associated nephropathy through Doppler ultrasound of renal vessels. The study focuses on research groups comprising individuals affected by this nephropathy. Dopplerography studies aim to evaluate the effectiveness of N-acetylcysteine in treating coronavirus-associated nephropathy. Doppler ultrasound helps assess renal blood flow and vascular parameters, providing insights into the functional state of the kidneys. The evaluation of N-acetylcysteine’s effectiveness suggests an exploration of its potential as a therapeutic intervention for coronavirus-associated nephropathy. This research contributes to understanding the renal implications of COVID-19 and potential treatment avenues through the application of Doppler ultrasound...
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Posted by admin on Nov 30, 2023 in |
The quest for new antimitotic drugs is an ongoing process where microtubules act as an attractive target for anti-cancer drugs. In this work, we report on the anti-cancer activity of Amlodipine besylate (AB), a salt derivative of the dihydropyridine groupdrug Amlodipine, a long-acting third-generation calcium channel blocker. Cytotoxicity assay revealed that AB demonstrated cytotoxic effects against cancer cells with greater specificity against cervical cancer cells (IC50 10 μM) whereas the non-cancerous NKE cells remained unaffected at this dose. AB inhibited HeLa cell migration as observed via scratch assay. Docking studies indicated that AB has the highest binding affinity for vinca site compared to Tubulin’s taxol, colchicine, and laulilamide binding domains. AB similarly interacted with tubulin to vinca and established two hydrophobic interactions with VAL353, PRO325; hydrogen bonds with ASN329, VAL175 and THR218 at the binding pocket. The stability of the Tubulin-AB complex was analyzed by molecular simulation studies. Further AB treated cells revealed altered microtubule architecture and drug-induced microtubule depolymerization in HeLa cells. More detailed study might unravel additional...
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