Posted by admin on Dec 1, 2014 in |
Herbal and herbo mineral preparations are being traditionally used in Indian system of Medicine especially in Siddha Medicine. Herbo mineral preparations have longer Shelf life. Rasa Chendooram (RC) is prepared as per classical text book of Prana Rakshamirtha Sindhu, for Vadha diseases. Before conducting clinical trial, Pre clinical study should be undergone as per WHO guidelines .The clinical trial has been approved by IEC (IEC NO-GSMC-CH/1/2013/011).The present preclinical study aims to carry out safety and toxicity of RC (IAEC NO.XXXXIX/07/CLBMCP/2013dated 29.06.2013) Adult both sex of Swiss albino rats weighing 220-250 gm were used. Acute & Sub acute toxicity were carried out as per OECD guidelines 423 and 407. Hematological Parameters, biochemical parameters histopathological study were performed for all animals. The study concludes that on oral administration of 100mg/kg of bodyweight of RC to Swiss albino rats, there was no change in behaviour movements and no characteristic clinical sign of toxicity or mortality...
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Posted by admin on Dec 1, 2014 in |
The combination of Nigella sativa seed oil and ginger extract is used as antiulcer agent and to treat abdominal pain, diarrhoea and flatulence. The paper describes validated simultaneous equation method for the simultaneous estimation of Nigella sativa seed oil and Ginger extract in a combined dosage form. Nigella sativa seed oil and Ginger extract were found to have absorption maxima at 256 and 282 nm respectively in 5% SLS solution. Both these drugs obeyed Beer’s law in the concentration range of 100-500μg/mL. The high values of correlation coefficients (r2) indicated good linearity of calibration curve for both the drugs. The results of analysis have been validated statistically and by recovery study the value of standard deviation ranging from 97-99.21 % for Nigella sativa seed oil and 98-101.6 % for ginger extract were indicative of the accuracy and precision of the proposed method. The proposed method was successfully applied for the determination of nigella sativa seed oil and Ginger extract in the pharmaceutical formulation. This method was found to be...
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Posted by admin on Dec 1, 2014 in |
Chonemorpha fragrans (Moon) Alston is a liana belongs to family Apocynaceae. Due to its ethanobotanical importance, it is used intraditional medicinal systems as a part of various medicinal preparations. In the work presented here the plant has been explored for its antioxidant potential and DNA protection ability. The antioxidant potential of C. fragrans was determined using DPPH, FRAP, Phosphomolybdanu and H2O2 Scavenging Capacity. Phenol and flavonoid content was measured followed byCorrelation analysis between antioxidant activities andphenols and flavonoid content. In addition, DNA protection ability of extracts was evaluated using double stranded plasmid DNA. C. fragrans extracts have shown significant antioxidant potential. Phenol content showed high correlation with Phosphomolybdenum reduction potential where as flavonoid content with Phosphomolybdenum as well as H2O2 radical scavenging potential. All the extracts exhibited an ability to protect the DNA against devastating effects of Fentons reagent except methanol roots, stem bark, in vitro and callus extracts. The extracts have shown significant antioxidant activityand Phenol, flavonoid content was found to contribute towards antioxidant potential of C. fragrans....
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Posted by admin on Dec 1, 2014 in |
Direct Compression is becoming a preferred manufacturing process in order to speed up the manufacturing and reduce costs. Although a major requirement for direct compression process is selection of excipients with the optimum physical characteristics that increase final flowability and compressibility of the ready for compression (RFC) tableting blend. By using combinations of excipients and novel processing techniques, particles / excipients can be engineered to deliver desired properties to be used in direct compression. The resulting engineered excipients are commonly known as “coprocessed,” “high functionality,” “multifunctional,” “high functionality,” “performance” excipients. Prosolv Easytab SP and Panexcea™ MHC 300G are amongst this type of high-functionality excipients, which are used in present study to check their comparative performance on compression of tablets using direct compression technique with use of BCS class– II drugs such as Vardenafil HCl (5 mg). The drug was characterized using Infra-red spectroscopy. Pre-formulation, solubility and drug-excipient compatibility studies were carried for drug substance. While for all individual batches, blend analysis and various IPQC parameters such as tablet hardness,...
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Posted by admin on Dec 1, 2014 in |
Cancer plays crucial role in human life and critical disease to cure. Signaling pathways involved in stem cells development process. Especially, Wnt/β-catenin signaling pathway plays important role in stem cells self-renewal, differentiation and proliferation. Despite, over expression and mutation of Wnt/β-catenin signaling pathway induced variety of cancer development. Anticancer activities of several natural and synthetic agents were used to treat many cancer types. Many synthetic agents were used to cure various cancer but they have their toxicity. So, plant derived chemotherapeutic agents are going to investigate against cancer without side effect. In this paper we evaluate anticancer properties of Tradescantia spathacea medicinal plant and aberrant expression of β-catenin protein in human breast adenocarcinoma cell line (MCF-7). The plant leaves were collected, shade dried and extracted with hydroalcohol using soxhlet. Cytotoxic activities were assayed with MTT colorimetric procedure against MCF-7 cell lines. From the result, plant leaves extracts showed nearly 50 % MCF-7 cell line inhibition at 229.7µg/ml tested...
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