Posted by admin on Dec 1, 2014 in |
A rapid, selective, precise, accurate, rugged and robust high performance liquid chrmatgraphic (HPLC) method for the simultaneous determination of Candesartan Cilexetil & Hydrochlorthiazide in tablet dosage form .The method was validated according to ICH and FDA guidelines. The chromatography is performed on a Kromasil C8, 150 x 4.6 mm, 5µm, in a gradient mode with a mobile phase of Water, acetonitrile and Trifluracetic acid in different ratios. UV-Visible detector at 285 nm was found to be suitable for detection. Linearity was observed in the range of 70 -130μg/ml with correlation coefficient of 0.9999. Sensitivity, accuracy, range, precision, robustness, ruggedness, stability, specificity, limit of detection, limit of quantification and system suitability parameters were validated for the developed method. The developed method was successfully applied to estimate the amounts in pharmaceutical formulations and for the stability indicating...
Read More
Posted by admin on Dec 1, 2014 in |
In the present work various conventional methods and microwave method for synthesis of substituted quinolone reported. Substituted anilines were taken as a starting material and reacted with ethyl acetoacetate in presence of catalytic amount of acid and cyclized by various cyclisation agents that give the title compounds which differ in its color intensity, reaction time as well in %yield. Based on results we obtain, it has been concluded that microwave method for synthesis of substituted quinolone is more convenient in terms of reaction time and use of...
Read More
Posted by admin on Dec 1, 2014 in |
Cucurbitaceae commonly known as “Guard family” is distributed throughout India. Most of them, are perennial climbers with attractive flowers. The family includes plants having food as well as medicinal values. The species of the family are collectively known as cucurbits. They grow wild as well as cultivated throughout the state in Odisha in general and Similipal Biosphere Reserve (SBR) forest and its adjoining areas in particular. The major cucurbits available in SBR and its adjoining areas are Coccinia grandis L., Cucumis melo L., Lufa acutangula (L.) Roxb., Luffa cylindrica (L.) Roem., Solena amplexicaulis Lam., Mukia madraspatana (L.) Roem., Trichosanthus cucumerina L., Trichosanthus tricuspidata Lour.and Diplocyclos palmatus (L.) Jeffrey. The food values of some of these cucurbits have been assessed. Phytochemical analysis and antibacterial activity of the plant parts have shown potent medicinal values. Present paper highlights the diversity of wild cucurbits (WC) at SBR along with their nutritional and medicinal...
Read More
Posted by admin on Dec 1, 2014 in |
Marine organisms are potentially prolific sources of highly bioactive secondary metabolites that might represent useful leads in the development of new pharmaceutical agents. The Libyan marine biodiversity including macroalgae remains partially unexplored in term of their potential bioactivities. Method: The phytochemical analysis of the alcoholic extracts of some commonly occurring seaweed Cystoseira compressa, Enteromorpha intestinals, Corallina and Ulva lactuca and their evaluated for antibacterial activity by well diffusion assay were studied. Four different solvents namely water, ethanol 99 %, methanol 99 %, and methylated spirit 95 % were used for extraction. Results: The phytochemical analysis revealed the presence of Carbohydrates, Steroids, Tannin & Phenols, Saponins, Proteins, and glycosides. The extracts were subjected for study of antibacterial activity. The zone of inhibition ranged between 8 to 16 mm in aqueous extract and up to 16 mm in methanol extract. The maximum activity (16 mm) was recorded from methanol extract of Ulva lactuca against Staphylococcus aureus and, minimum activity (8mm) recorded by Cystoseira compressa against Staphylococcus...
Read More
Posted by admin on Dec 1, 2014 in |
Objectives: To evaluate the antidepressant activity of fresh leaves of Tinospora cordifolia in albino mice. Materials and Methods: Swiss albino mice weighing 25-30grams of either sex were selected for the study. Six animals were allocated to each experimental group. Animals were divided into 4 groups of 6 mice each. Group 1 – Control group treated with normal saline (0.1ml/10gm), Group 2 received standard group treated with 15mg/kg of imipramine, Group 3 – T1 treated with Tinospora cordifolia fresh leaves (100 mg/kg) and Group 4 – T2 treated with Tinospora cordifolia fresh leaves 50 mg/kg and 10 mg/kg of imipramine. Decrease in total immobility period denoted antidepressant activity in both tail suspension method & forced swim test in albino mice. Results: The test drug that is 50mg/kgp.o of Tinospora cordifolia fresh leaves along with 10mg/kg p.o of imipramine significantly decreased the total immobility period in both forced swim test and tail suspension method in albino mice. In forced swim test the percentage decrease in the immobility period was 38.68% when...
Read More
