Posted by admin on Dec 1, 2014 in |
Quorum sensing is a bacterial cell communication mechanism mediated through signal molecules such as AHL’s. Many opportunistic pathogens use these quorum sensing circuits to coordinate their virulence gene expression. Wide range of naturally occurring substances particularly plant extracts have been evaluated for their ability to modulate quorum sensing in Gram-negative bacteria. Preliminary screening of popular food spices for QSI activity revealed that Cinnamomum verum (Dalchini) had good QSI activity. Recognizing its medicinal value and dietary consumption, an attempt was made to evaluate QSI activity of Cinnamomum verum on bacterial pathogens like Pseudomonas and Serratia and confirmed by using bioindicator organism Chromobacterium violaceum. Cinnamomum verum extract had more QSI activity than the antibacterial activity in Serratia. In Pseudomonas, the antibacterial activity was more while QSI activity was comparatively less. Interestingly spice extract could reduce swarming nature in both the organisms, which is one of the important virulent factor regulated by QS. The phytochemical components in the crude extract were analyzed by qualitative methods. MS analysis of the treated samples revealed...
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Posted by admin on Dec 1, 2014 in |
A HPLC method has been described for simultaneous determination of Chlorpheniramine Maleate, Phenylpropanolamine Hydrochloride and Paracetamol in formulation. This method is based on HPLC separation of the three drugs on the Thermo Hypersil Gold C18 column(250mm ×4.6mm, 5.0µ), with isocratic conditions and mobile phase containing methanol: 0.01M disodium hydrogen phosphate dihydrate buffer pH 7 adjusted with Ortho Phosphoric Acid (OPA) (60: 40) at a flow rate of 1mL/min using UV detection at 217 nm. This method has been applied to formulation without interference of excipients of formulation. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 0.5-3µg/mL for Chlorpheniramine Maleate, 7-12µg/mL for Phenylpropanolamine hydrochloride, and 0.4-1.4µg/mL for Paracetamol, respectively. The mean values of the correlation coefficient, slope and intercept were 0.999 ± 1.72, 28455 ± 1.01, 26185 ± 1.28 for Chlorpheniramine Maleate, 0.999 ± 0.34, 23604 ± 1.16, 73758 ± 1.49 for Phenylpropanolamine hydrochloride and 0.999 ± 0.80, 51233 ± 1.89, 5560 ± 1.62 for Paracetamol respectively. The method...
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Posted by admin on Dec 1, 2014 in |
Various parts of Lasia spinosa (Linn.) are widely used in North East region to manage wide range of disease but so for no scientific study was done to find out its pharmacological properties which may support its use in traditional medicine. Bioactive fraction (ethyl acetate fraction, LSE) from the methanol – water (80:20) extract of Lasia spinosa rhizome was evaluated for gastroprotective and antioxidant activity. In the safety evaluation, LD50 was found to be more than 2000 mg/kg. High performance thin layer chromatography (HPTLC) finger printing of the total extract was also carried out using gallic acid as markers in an attempt to characterize the constituents responsible for the activities and also to standardize the extract. The bioactive fraction from Lasia spinosa rhizome (LSE; 50 and 100 mg/kg body weight) was administered orally, twice daily for 5 days for prevention from Ethanol (EtOH)-, cold-restraint stress (CRS) – and pylorus ligation (PL)-induced ulcers. Estimation of antioxidant enzymes activity was carried out in CRS-induced ulcer model. LSE showed dose-dependent ulcer protective...
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Posted by admin on Dec 1, 2014 in |
This study focused on an investigation of dissolution property of solid dispersion system consisting of drug, exipients, and carrier. The purpose of this study was to enhance the dissolution of Atorvastatin Calcium, a practically water-insoluble drug by preparation of solid dispersion using hydrophilic polymers and a carrier PEG 6000. Solid dispersion formulations were prepared by using The Melt Solvent and physical mixing method. Physical mixtures (PMs) of atorvastatin and hydrophilic polymer Kollicoat IR and PVK 30 were prepared at 1:0.5, 1:1 and 1:2 ratios. Melt Solvent method was used to prepare solid dispersion of atorvastatin, PVK 30 and kollicoat IR at 1:2 ratio. Solid dispersions were characterized by differential scanning calorimetry, scanning electron microscopy and dissolution tests. Characterization studies revealed that solid dispersion of Atorvastatin prepared by Melt Solvent methods showed better dissolution compare to Physical mixing and pure atorvastatin due to the conversion of atorvastatin into a less crystalline and/or amorphous form. The order of dissolution enhancement was Kollicoat IR > PVK30 in solid dispersions as well as...
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Posted by admin on Dec 1, 2014 in |
Anti-obesity effect of Cinnamaldehyde and Orlistat on High fat diet induced obese rats was investigated. 30 male wistar albino rats were divided into 5 groups: control group fed on normal standard diet; HFD group; Orlistat (HFD+ 50mg/kg body weight; CA (HFD+ 40mg/Kg body weight) and CA (HFD+ 80mg/Kg body weight) for 8 weeks. The anti obesity activity of CA and OR was estimated in terms of Food intake, body weight gain, feed efficiency ratio, lipid profile, AI, CRI and faecal lipids. Obesity induced group of rats showed significant (p< 0.001) increase in body weight, feed consumption, TG, TC, LDL-c, VLDL-c in serum, AI, CRI and decrease with HDL-c level (p< 0.001) compared with the normal group of rats. The BWG and FER were reduced in CA treated rats. CA treatment also resulted in significant (p< 0.001) decreases in serum TC, TG, LDL-c, AI and CRI and increased (p< 0.001) HDL-c concentrations in a dose dependent manner compared with untreated obese rats. The results were comparable with Orlistat, a standard...
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