Posted by admin on Dec 1, 2014 in |
An accurate, precise, specific and rapid RP-HPLC method was developed and subsequently validated for the determination of Dapagliflogin in API. Better separation of the drug was achieved on BDS column (250×4.5mm, 5µ) with a mobile phase consisting of a mixture of ortho phosphoric acid and acetonitrile (45:55 v/v) at a flow rate of 1ml/min, with detection at 245nm using Photo Diode Array (PDA) detector. The developed method was validated for different parameters such as linearity, accuracy, precision, limit of detection(LOD), limit of Quantitation (LOQ), robustness and the results were found to be within the limits according to ICH (International Conference on Harmonization) guidelines. The retention time was found to be 2.963 min. The method was found to be linear in the range of 25-150µg/ml with a correlation coefficient (r2) of 0.999. The LOD and LOQ of the method were calculated to be 0.6 and 1.8µg/ml respectively. The Precision was estimated by employing repeatability; intra-day and inter-day studies and the results were calculated as %RSD values and were found to...
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Posted by admin on Dec 1, 2014 in |
Objective: Potato (Solanum tuberosum L.) is a major world food crop next in production to maize, rice and wheat. Potatoes are a source of dietary energy due to their carbohydrate level and also contain a high value of vitamins. The objective of this study was determine the phytochemical constituents and antimicrobial activities of four common potato varieties developed by CPRI (Central Potato Research Institute, Shimla, India) i.e. Kufri Bahar, Kufri Badshah, Kufri Pukhraj and Kufri Puskar. Methods: The antimicrobial activity of selected plant spices against the mentioned microorganisms and their effectiveness were assessed by the presence or absence of inhibition zones and zone diameter by using Agar well diffusion technique and some chemical tests were performed for analysis of phytochemical constituents. Results: The antimicrobial activity of all the potato extracts showed the significant inhibitory activity against all the bacteria and fungi strain. Conclusion: The demonstration of broad spectrum activity of potato varieties may help to discover new chemical classes of antibiotic substances that could serve as selective agents for...
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Posted by admin on Dec 1, 2014 in |
A number of trisubstituted 2-pyrazolines which are known for their biological importance like antimicrobial, anti-inflammatory, antitubercular, analgesic, anticancer, antimalarial, anti-viral, antihelmentic activities are prepared by reacting chalcones with phenyl hydrazine in absolute ethanol in presence of pyridine. In the present investigation an attempt has been made for the synthesis of 2-pyrazoline derivatives and subjected to molecular properties prediction by molinspiration, molsoft and osiris softwares. The synthesized compounds have been confirmed by IR, Mass and 1HNMR spectral data. These compounds were also screened for various biological activities like antifungal, anti-inflammatory and acute toxicity activity by standard methods. The synthesized compounds have shown moderate to good antifungal, anti-inflammatory activity and some of the synthesized compounds have shown significant activity as compared with standard. Compound 5d showed good antiinflammatory activity, compounds 5c, 5d, and 5e showed higher antifungal activity and all the synthesized compounds were found to be in conformity with lipinski’s...
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Posted by admin on Dec 1, 2014 in |
Baccharis trimera is used in traditional medicine in South American countries for treatment of diseases, such as digestive disturbances, rheumatism, diabetes and inflammatory processes. Given its pharmacological potential, the aim was to characterize the vegetal drug B. trimera and attain and standardize its lyophilized extract to use as raw vegetal material in the development of phytotherapeutic medicine. Chemical identification and prospecting, granulometric determination, loss by desiccation, total ash and caffeic acid through High-Performance Liquid Chromatography were carried out. The lyophilized extract obtained was submitted to assays for determining caffeic acids, rheology, hygroscopicity, solubility, superficial area, porosity, particle size through laser granulometry and the thermogravimetric curve. The assays demonstrated that the sample is within the described specifications in literature and, therefore, is adequate to obtain the liquid extract and later dry and standardized in relation to the content of caffeic acids. The lyophilized extract was fine powder, demonstrating the necessity for addition of excipients that make possible the use of the input for the attainment of solid pharmaceutical forms. It...
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Posted by admin on Dec 1, 2014 in |
The interaction of omeprazole with different types of antibiotics was investigated in biological buffer at physiological pH=7.4 by using electrochemical (cyclic and linear sweep voltammetry) method. The reduction processes of omeprazole in the absence of and in the presence of azithromycin, clarithromycin, roxithromycin and doxycyclline are irreversible. Omeprazole interacts in solution with antibiotics structures by a predominantly electrostatic mechanism. From electrochemical data, The 1:2, 1:1, 1:1 and 1:2 complexes of azithromycin-omeprazole, clarithromycin-omeprazole, roxithromycin-omeprazole and doxycycline-omeprazole are formed with the binding constants β = 3.930 (±0.15), 0.985 (±0.02), 0.321 (±0.01) and 8.230 (±0.21) µM-1 respectively. The results show that, the binding affinity of omeprazole increases in the sequence: roxithromycin > clarithromycin > azithromycin >...
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