Posted by admin on Nov 1, 2014 in |
Asthma is a multi factorial disease characterized by airflow obstruction, wheezing and dysonea and for this many genes are responsible. In this study, an attempt has been made to identify genes along with the proteins encoded by them which are responsible for Asthma along with clinical trials. In the practice of Ayurveda, it’s well established that shirish (Albizia lebbeck) can be used as a treatment of Asthma. However, how the compounds of shirish bark functions at the molecular level is yet unknown. The present study shows an insilico molecular interaction studies and clinical trials as well for the compounds of shirish bark. The results show how the compounds of shirish bark affect the target proteins of asthma which actually will aid in the design of putative inhibitor. Among the screened 9 (nine) compounds of shirish, all the compounds are giving good binding affinity to the selected proteins and among them lebbecacidin interacts potentially and is also showing lowest binding energy with IL-5, one of the target proteins. Lebbecacidin has...
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Posted by admin on Nov 1, 2014 in |
Nifedipine loaded mcoadhesive micropshres were prepared by o/w/o emulsification cross linking method using natural polymer chitosan. Microsphres with particle size range b/n 50-300 µm were obtained by this method. The prepared microspheres were evaluated for its drug loading, surface properties, in-vitro release profile. The diffusion and permeation characteristic of the drug is studied by using cellophane membrane and excised porcine intestinal mucosa respectively. The mucoadhesion properties of the microsphers were determined by using modified USP disintegration test apparatus model. In vivo animal studies were studies using rabbit model indicated a controlled blood level profile in comparison with pure nifedipine. IR studies indicated no appreciable drug excipient...
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Posted by admin on Nov 1, 2014 in |
Surgeries like vasectomy, tubectomy, and medication (contraception) for the population control are not fully restraining the population growth. Medicine available to control birth rate carrying side effects. The attempt of exploring the importance of Ayurveda the present work was carried out. The methanol extract of the plant Elytraria acualis was prepared by maceration technique. Extract affects fertility by reducing the sperm count and motility in the albino rats. The male albino rats were divided into three groups of eight animals in each. The first group was considered as control and Group II, III was treated with dose 200mg/kg and 300mg/kg extracts respectively. The treatment of the extract was carried out by gastric gavage for 21 days through oral. On 22nd day all rats were sacrificed to study sperm count and motility. The blood samples were centrifuged to separate serum. The serum samples used for the serological parameters. The reduced levels of vital reproductive organs weights and sperm count, motility were observed in the treated rats compared to control. The...
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Posted by admin on Nov 1, 2014 in |
To synthesize structurally modified novel Glycogen Synthase Kinase-3βinhibitors to overcome insulin resistance in type 2 diabetes mellitus and to overcome associated health problems with reducing morbidity, mortality and economic costs of diabetes. Methods. Nineteen Phenylmethylene hydantoin analogs were designed and docked in the ATP binding site of GSK-3β by Molegro Virtual Docker 2006.1.5. The synthesis of proposed six hydantoin analogs were performed based on docking results by two approaches, Knoevenagel condensationand Steglich Esterification reaction, keeping the hydantoin ring and different ester substitution at benzylidene ring system can afford potent and selective GSK-3β inhibitors involved in the control of glycogen metabolism. The antidiabetic activity and liver glycogen content were also determined against Streptozotocin induced diabetic rat model. Results. The synthesized compounds H-a, H-c and H-e increased hepatic glycogen content in range 410-420 mg/gm liver weight while compounds H-b, H-d and H-f cause increased in hepatic glycogen content in range of 357-370 mg/gm liver weight as compared to control having 242.01 mg/gm liver weight. Conclusion. The synthesized compounds decreased blood glucose...
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Posted by admin on Nov 1, 2014 in |
The inhibitory effect of Eucalyptus globulus oil was evaluated against the E. coli and the minimum inhibitory concentration (MIC) value of Eucalyptus globulus oil was calculated by agar dilution assay, which was found to be 1µl/ml. The E. coli biofilm was formed on catheter surface and quantified by crystal violet staining system. The crystal violet quantification method showed maximum biofilm after 48h. The effect of Eucalyptus globulus oil was evaluated against E. coli biofilm and was found to inhibit the biofilm by 62%. To evaluate the effect of oil containing silica nanoparticle against biofilm system, the E. globulus oil was loaded in the silica nanoparticle. The synthesized nanoparticle (SNP) was characterized using scanning electron microscopy (SEM), and data showed the average size of silica nanoparticle 1000 nm. The application of oil loaded silica nanoparticle was done against E coli biofilm system and biofilm quantification assay showed 81% reduction in biofilm. The inhibition was further confirmed using light microscopic analysis of biofilm after staining with 0.5% crystal violet. The study...
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