Posted by admin on Sep 1, 2014 in |
Objective: To study the nephroprotective activity of Bauhinia purpurea unripe pods and bark against gentamicin-induced nephrotoxicity. Materials and Methods: Twenty four healthy adult albino rats of either sex (150-200 g) were randomly divided into four groups of six animals each. Group, I served as vehicle control and Group II as the negative control (gentamicin 80 mg/kg/d, i.p). Groups III and IV (test groups) were administered ethanolic extract of Bauhinia purpurea bark (BBE) and unripe pods (BPE) in the dose of 400 mg/kg/day, one hour before gentamicin administration. All the treatments were given for 8 days. On the 9th day, blood and urine samples were used for biochemical analysis and kidneys for histology and assessing anti-oxidant activity. Results: Gentamicin caused nephrotoxicity as evidenced by highly significant (p<0.001) elevation of blood urea, serum creatinine and urine glucose in Group II as compared to Group I. Also administration of Gentamicin in group II caused oxidative stress in rats suggested by significant increase in MDA level and significant decrease in Catalase and Reduced...
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Posted by admin on Sep 1, 2014 in |
The kinetics of oxidation of quinine sulphate [8α, 9R-61– methoxycinchonan- 9 – ol sulphate], (QS) by Chloramine-B (Sodium N-Chlorobenzene Sulphonamide, CAB) has been studied in aqueous hydrochloric acid medium at 303 K. The reaction rate shows a first order with respect to [CAB], fractional order with respect to [HCl] and [QS]. Activation parameters were evaluated from the kinetic data at different temperatures. Negative entropy of activation indicated the involvement of a rigid complex in the activated state. The dielectric constant of the medium has a small effect on the rate. Ionic strength and the reduction product benzene sulphonamide [BSA] do not affect the reaction rate. The solvent isotope effect is studied. The reaction products are identified and characterized by spectral (IR and NMR) data. The proposed reaction mechanism and the derived rate equation are consistent with the observed kinetic...
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Posted by admin on Sep 1, 2014 in |
Overproduction of free radicals can cause oxidative damage to biomolecules (e.g., lipids, proteins, DNA), eventually leading to many chronic diseases, such as atherosclerosis, cancer, diabetes, aging, and other degenerative diseases in humans. Plants may contain a wide variety of free radical scavenging molecules, such as phenolic compounds, nitrogen compounds, vitamins, terpenoids, and some other endogenous metabolites, which are rich in antioxidant activity. In the present study in-vitro antioxidant potential of various fractions of Hibiscus rosa sinensis petal (HRS) ethanolic extract was investigated. Fractions prepared from crude ethanolic extract of HRS were concentrated and used for various in-vitro assays. Ethyl acetate fraction of HRS (EHRS) had a very high content of total phenolics and flavonoids. DPPH radical scavenging activity (IC50-9µg/ml) of EHRS was comparable with standard Quercetin. Superoxide, ABTS, and hydroxyl radical scavenging activities of EHRS were also found higher than other fractions. The total antioxidant activity and reducing power were also high in EHRS. So the study suggests that EHRS may be a potential source of bioactive substances with...
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Posted by admin on Sep 1, 2014 in |
ABSTRACT: Celastrus paniculatus is a known herb used in alternative system of medicines since ancient times. Traditionally seed oil of Celastrus paniculatus has been in various medicinal remedies to cure neuronal disorders, asthma, cough, etc. The constituents found in Celastrus paniculatus reportedly are sesquiterpenoids, triglycerides, triterpenoids. A high-performance thin layer chromatography, which is considered as a sensitive method for accurate quantification method for analysis of compounds. The instrument CAMAG Linomat 5 lamp was used, and the wavelength was 254...
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Posted by admin on Sep 1, 2014 in |
Local traditional healers in the Great Lakes Region used to administer Hypoestes triflora (Roem) leaf decoctions to treat patients with anemia and liver diseases, and also to boost immunity in HIV patients. This study was designed to evaluate, in guinea-pigs, the antianemic and hepatoprotective potentials of this plant. Anemia was induced with phenylhydrazine hydrochloride and hepatotoxicity with paracetamol. Animals were divided into normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced animals treated with H. triflora aqueous extracts and R ill-induced animals treated with an Iron solution for anemic animals or silymarin for hepatitis animals. Dosing was made as 1-day single dose or 7-days repeated dose. In anemic animals, the production rate of RBC was significantly (p<0.001) higher in the T group as compared to the C group. In paracetamol-induced hepatitis animals, the plant extract exhibited about 80-90% protective effect in T group as compared to the C group. The effects were comparable to or higher than R responses. H. triflora leaf extracts have...
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