Posted by admin on Sep 1, 2014 in |
In the present study, a simple, precise, and accurate method was developed and validated for analysis of Ketorolac Tromethamine in Tablet formulation. A gradient HPLC analysis was performed on Grace C18 column (250 cm × 4.6 mm × 5 µ). The compound was separated with a solvent mixture of Methanol and water in a ratio of 65:35 v/v with 0.1% O-phosphoric acid as the mobile phase at a flow rate of 1ml/min. The UV detection was performed at the ʎ max 245 nm. The retention time was found to be 7.70 min. The system suitability parameters such as theoretical plate count, tailing factor, and percentage relative standard deviation (RSD) between six standard injections was within the limit. The method was validated according to the International conference of harmonization (ICH) guidelines. The linearity was found to be in the concentration range of 5-25 µgm/ml as indicated by correlation coefficient (r2) of 0.999. The robustness of the method was evaluated by deliberately altering the chromatographic condition. The developed method can be...
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Posted by admin on Sep 1, 2014 in |
Inflammation is a primary physiological defense mechanism that helps the body to protect itself from infection, toxic chemicals, or other noxious stimuli. The methanolic seeds extract of Brassica juncea was evaluated for its anti-inflammatory activity, in-vivo methods. Anti-inflammatory drugs with low toxicity and higher therapeutic values. It is a defensive mechanism of the body to remove the injurious stimuli as well as initiate the healing process for the tissue Brassica juncea has been used since ancient times, and it is popularly known as mustard. Present study aimed to evaluate the anti-inflammatory activity of methanolic extract of Brassica juncea against carrageenan-induced paw edema test at different doses (500 and 1000 mg/kg body weight) of the methanolic extract. At the dose of 1000 mg/kg body weight, the extract showed significant anti-inflammatory activity in the carrageenan-induced edema test models in rats showing 65.98% reduction in the paw volume comparable (P<0.05) to that produced by the standard drug indomethacin 81.96% at 5 hours respectively. The results of this study explicate justification of the...
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Posted by admin on Sep 1, 2014 in |
The rise of antibiotic-resistant pathogens leads to the development of new antibiotics and drugs. Natural products capture the attention of the researchers for such drug discovery. Apart from medicinal plants, many microorganisms also play a great role in the discovery of antibiotics. In this context, bacteria from Sea surface microlayer (SML) are also investigated nowadays. The secondary metabolites of bacteria from such extreme environment are expected to produce some effective metabolites which may have potential activity against pathogens. In the present study, B. subtilis was isolated from SML and different crude extracts of the bacteria was tested against human pathogens. The results declared that ethyl acetate extract has efficient activity rather than methanolic extracts. The common opportunistic pathogen E. coli was found highly susceptible to the extract followed by the other pathogens. The present study will pave the way for further studies on purification of crude extract and testify them for other biomedical applications like anticancer...
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Posted by admin on Sep 1, 2014 in |
A series of new Baylis–Hillman derived cinnamyl substituted quinazolinone derivatives have been synthesized by the condensation reaction between Baylis-Hillman bromides and quinazolinones in a simple and efficient manner in less reaction time with high yields. Methyl quinazolinone yields two isomers of Baylis-Hillman derived 3–N-cinnamyl substituted quinazoline-4-one derivatives and 4-O-cinnamyl substituted quinazoline derivatives, and the structures were confirmed by X-ray crystallography. All the newly synthesized compounds were characterized by their spectral data and evaluated their antibacterial and antifungal activity. The compounds (3a, 4a) without any substitution at the aryl group exhibited good antibacterial activity especially on S. epidermidis (gram positive), Replacement of hydrogen atom of the aryl group by CF3 (3c, 4c) or by fluorine (3g) exhibited significant antibacterial activity on both gram-positive (S. epidermidis) and gram-negative (K. pneumonia) organisms compared to other compounds. All the compounds exhibited interesting antifungal activity on C. albicans microorganism exclusively and inactive on the remaining organisms. Compound 3a and 4f exhibited good antifungal activity compared to the other...
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Posted by admin on Sep 1, 2014 in |
Flavones are an important class of flavonoids having a wide range of biological activities like antioxidant, anti-inflammatory, anti-oestrogenic, antimicrobial, anti-allergic, antioxidant, vascular, antitumor, and cytotoxic activities. First cyclin-dependent kinase inhibitor, flavopiridol, which is a flavone is approved as an orphan drug in Europe for the treatment of relapsed chronic lymphocytic leukemia. So, these structural motifs may show potential activity to wide therapeutic conditions. Thereby synthesis of flavone is an important aspect in medicinal chemistry. This article covers the synthetic strategies available in the literature and their usefulness in terms of yield and reaction condition and the biological activity exerted by the natural and synthetic flavone derivatives and their probable mechanism of action. It is expected that this review may be helpful for medicinal chemist in research regarding flavones with new therapeutic...
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