Posted by admin on Aug 1, 2014 in |
The aerial parts of Scoparia dulcis Linn. belonging to family Scrophulariaceae. Traditionally are used as antiulcer, antibacterial, antifungal, cytotoxic, antimicrobial, antianaemic, analgesic, antidiabetic, hyperlipidaemia, hepatoprotective etc. From literature survey the aerial parts of Scoparia dulcis Linn. are rich source of flavonoids, tannins, phenolic compounds but not yet scientifically investigated for nephroprotective activity. So in order to give scientific background the aerial parts of Scoparia dulcis Linn. was evaluated for nephroprotective activity by gentamicin induced nephrotoxicity. The present study carried out with Pharmacognostic examination like macroscopic, microscopic, proximate values and preliminary phytochemical test evaluation of alcohol and water extract; And their Pharmacological evaluation that gentamicin at dose of 100 mg./kg. BW/day I.P. rout produces significant nephrotoxicity as evidenced by decreased in body weight, urine volume & elevate in kidney weight, serum urea, uric acid, serum creatinine, with renal tubular necrosis. After treatment the alcohol and water extracts each (200 mg./kg. B.W./ day I.P.) of aerial parts of Scoparia dulcis Linn. Show significant decreases (p˂0.001) serum urea, uric acid, creatinine level,...
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Posted by admin on Aug 1, 2014 in |
In the present work, efforts have been made to model the antibacterial activity of 4-thiazolidone derivatives against pathogenic bacteria P. aeruginosa by using QSAR (quantitative structure-activity relationship) methodology. Multivariate analysis gave excellent model which was tested using cross validation using leave one out method. The cross- validation method was applied to the data set in order to prove the predictive power of statistically significant QSAR models, which help to explore some expectedly potent compounds. The best model predicting the antibacterial activity indicated that the 2D auto-correlation, 3D and WHIM parameters such as MATS8p, RDF070u, RDF035e, Mor30v (3D) and E1u (WHIM Parameter) are very effective in describing the antibacterial activities of these compounds. The study revealed that E1u, MATS8p, RDF035e and RDF070u contribute positively whereas contribution of Mor30v contributes negatively to the antibacterial activity. The compounds with improved antibacterial potential can be successfully designed with selected quantitative structure activity relationship...
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Posted by admin on Aug 1, 2014 in |
Micronutrient deficiencies such as potassium, magnesium and calcium are very common in the general population and it is more predominantly present in the individuals with hypertension and cardiovascular patients. These deficiencies will have a massive impact on present and future cardiovascular health outcomes such as hypertension, myocardial infarction, stroke, etc. Recent efforts to reduce the prevalence of hypertension have focused on non-pharmacologic means, specifically diet in order to overcome micronutrient deficiencies. From most of the studies it is evident that an increased intake of minerals such as potassium, magnesium, and calcium by dietary means to reduce blood pressure in patients with hypertension. This article also focuses on how these minerals influence vaso dilation, rennin- angiotension and aldosterone system, natriuresis, sympathetic nervous system nitric oxide production and vascular injury. On the whole, this review will talk about the roles of potassium, magnesium, and calcium in the prevention and treatment of hypertension with specific emphasis on clinical trial evidence, mechanism of action, and recommendations for dietary intake of these...
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Posted by admin on Aug 1, 2014 in |
Biological/Toxicological activity of Cobra venom (CV) is usually estimated in terms of LD50 which varies at each time of test, when stored in liquid condition at non-native environment, as the CV contains most unstable complex proteins in it. Prepared & stored working solution of cobra venom at 5±3ºC in liquid condition for experimentation without any stabilizing agent, it becomes unstable and show huge variations in LD50 test result (performed on Swiss albino mice, weight: 17-22g). Such variations in biological activity of CV have adverse impact on venom dependent potency test of anti-snake venom sera (ASVS) which has direct commercial value. In this study, working stock solution of CV was prepared in the three different diluent sets (B, C and D) of solutions commonly used in laboratories and stored at 5±3ºC, which was tested for LD50 at pre-defined interval, up to 120 days. Obtain results were statistically calculated for variation from mean value in terms of Standard deviation (SD).The study result indicates that if the working stock solution of CV...
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Posted by admin on Jul 31, 2014 in |
Background &Objective: To explore possible protective effect of neem leaf extract against methotrexate induced genotoxicity in the somatic cells (wing primordial) of Drosophila melanogaster. Methods: In the first phase of the experiment, neem leaf extract (NLE) at two doses was tested in the somatic cells (wing primordia) of Drosophila melanogaster (3rd instar trans heterozygous larvae resulting from cross of two bioactive strains) to probe effect on mutagenicity. In the second phase, effect of NLE at two doses (2.5% & 5%) was studied to probe its role in prevention of methotrexate induced genotoxicity. Concurrent negative control was run simultaneously in each experiment, where the larvae were exposed to the solvent (distilled water). The effect was observed and quantified under microscope as the number of spots (Clones) per wing. Results & interpretation: Only higher concentration of NLE (5%) showed significantly higher spontaneous mutation (2.7%) compared to untreated control group. Methotrexate induced 12.01 spots per wing (positive control). NLE at both doses (2.5 % & 5%) when administered concurrently with methotrexate, average...
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