Posted by admin on Nov 1, 2014 in |
The present study was aimed to evaluate in-vitro anti-cancer activity of ethanolic extract of Crataeva nurvala Buch. Ham. The method used for determining the anticancer activity was the MTT bio-assay using A549(Human Lung Carcinoma) cell line, Hela (Human Cervix)cell line and MDA-MB(Human Adenocarcinoma, Mammary Gland)cell line. The ethanolic extract of Crataeva nurvala bark at different concentration (10µg, 20 µg and 30 µg) was used as test for this bioassay. Vincristine30µg/ml (anticancer drug) was used as reference standard and blank as control. Optical density and % cell lysis was estimated at the end of the study. Extract showed promising anticancer effect. % Cell lysis was found to be in the range nearer to the reference standard for all the cell lines and optical density at 492nm is (0.575to 1.191) almost equal to that of reference standard i.e. 1.151 and always more than blank. The IC50 values were found to be less than 10μg against A549 cell line, the activities against HELA cell line were found to be 13μg and IC50...
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Posted by admin on Nov 1, 2014 in |
Plant derived natural products such as flavonoids, terpenes, alkaloids and polysaccharides have received considerable attention in recent years due to diverse pharmacological properties including cytotoxic and cancer chemo preventive effects. From a long period of time medicinal plants or their secondary metabolites have been directly or indirectly playing an important role in the human society to combat diseases. Oxidative stress results from an excessive production of reactive oxygen species beyond the body’s antioxidant capacity. The over production of reactive oxygen species can lead to damage to cellular biomolecules, which is implicated in the development of many diseases including cell death. The reactive oxygen species can be eliminated /deactivated by a number of antioxidants which include enzymic and non-enzymic antioxidants. The plant selected for the present study is Cucumis melo (Family: Cucurbitaceae) commonly known as Musk melon.Cucumis melo helps in increasing appetite, weight loss, urinary tract infections, constipation, acidity and ulcers. The goat liver was selected as the mammalian tissue to determine the antioxidant effect of ethanol extract in the...
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Posted by admin on Nov 1, 2014 in |
Accurate and sensitive assay methods for quantitative determination of Rutoside trihydrate (rutin), Trypsin and Bromelain in tablet formulation based on UV-spectrophometric analysis were developed and validated. This three drug combination has anti-inflammatory properties and hence is used for treatment of edema and inflammations. Rutin was analyzed directly by UV-Vis spectrophotometer at 257nm because Trypsin and Bromelain are not UV-active therefore they don’t interfere in the analysis of rutin. Trypsin and Bromelain being proteolytic enzymes, Casein was used as substrates for their analysis. Casein was standardized according to Bradford assay using Bovine serum albumin as a standard. Casein was hydrolyzed to amino acid tyrosine by both the proteolytic enzymes. Tyrosine formed was further reacted with Folin-Ciolcalteu (F-C) reagent and absorbance was recorded at 785nm. Trypsin was inhibited by specific trypsin inhibitor Nα-p-tosyl-L-lysine chloromethyl Ketone (TLCK) and specific proteolytic activity of Bromelain was analysed using casein. Bromelain was inactive towards N-a-benzoyl-DL-arginine p-nitroaniline (BApNA) therefore; BApNA was used as a specific substrate for analysis of Trypsin. BApNA was hydrolyzed to pNA by...
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Posted by admin on Nov 1, 2014 in |
The pH-sensitive microcapsules were prepared by interfacial polymerization, in which caffeine was used as a model drug and hyperbranched poly L-lysine (HBPL) and terephthaloyl chloride as wall-forming materials. The microcapsules were characterized by Malvern particle size analysis, scanning electron microscopy, Fourier transforms infrared spectrophotometry (FTIR) and UV-vis spectrophotometer. Triton X-100 was used as the emulsifier of interfacial polymerization. The effects of the emulsifier content on the particle size distribution, loading content, encapsulation efficiency and drug release property of microcapsules were discussed in detail. The release behaviors of caffeine in different phosphate-buffered saline (PBS) pH were investigated systematically. The FTIR analysis of caffeine-containing microcapsules demonstrated that caffeine was successfully encapsulated in the wall-forming materials. The resultant microcapsules had narrower particle size distribution, smoother surface, and higher drug release amount with the percentage weight of emulsifier being 1.5 wt%. The drug-release behavior of caffeine from microcapsules was evaluated as a function of pH. More than 87.1% caffeine was released into pH 7.6 PBS after 100 min, whereas only 37.0% caffeine was...
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Posted by admin on Nov 1, 2014 in |
The uses of plant medicines are very common in Asia and developing countries. Phytochemicals has been used to treat chronic as well as infectious diseases. Cardiospermum halicacabum Linn. (Sapindaceae) is an herbaceous climber, commonly found throughout India, used as vegetable as well as traditional medicine for rheumatism, lumbago and nervous diseases. Many pharmacological studies have been done with various parts of C. halicacabum plant to demonstrate its medicinal effect. The present work was aimed to isolate the active compounds from the leaf extract of C. halicacabum. The hydro alcoholic leaf extract (80% methanol + 20% distilled water) was subjected to isolation procedures for identification of active compounds. Eleven compounds were got and in vitro anti oxidant analysis were done with the isolated compounds.Compound 1 and 8 selected for structural elucidation. The NMR spectrum showed characteristic peaks of compound 1 and 8 and the active phytoconstituents were identified as Kaempferol- 3- o- α- l- rhamnoside and Apigenin – 7- o- β – d- glucuronide by structural elucidation. The isolated compounds...
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