Posted by admin on Aug 31, 2023 in |
Among different neoplastic lesions, hepatocellular carcinoma plays a pivotal role due to the extreme difficulty of treating it in advanced conditions. Research shows that there is a high intractability towards chemotherapy for this disease. Therefore, using medicinal plants can be another approach to fend off this grim scenario and pave a new way to prolong survival time. For this purpose, the ethanolic extracts of leaves and fruits of Cassia fistula (Golden shower) as a probable anticancer drug have been administered in the liver cancer cell line, HEPG2 and the gene expression of cytokines (interleukin-6, interleukin-8, interleukin IL-1β, transforming growth factor-TGF-β1, tumour necrosis factor-TNF-α) against this treatment has been analyzed in the present work. The current study revealed a significant reduction in the expression of pro-inflammatory cytokines by the application of these extracts demonstrating their vital role in suppressing tumour growth and proliferation in liver cancer. It appears that important phytochemicals played a key role in decreasing the expression of these cytokines. The combination of leaves and fruit extracts of Cassia fistula can be...
Read More
Posted by admin on Aug 31, 2023 in |
Plants have been widely adopted as the source of all drugs; this field has gradually broadened in developing countries where traditional medicine is essential in health care. Flaxseed is the seed from the plant Linum usitatissimum, which is an annual plant. This plant has attracted man’s attention for its advantages for human health. Plant materials contain a lot of bioactive phenolics, which demonstrate biological activity, including anti-radical, antioxidant, anti-microbial, and anti-cancer effects. Antioxidants are synthetic or natural compounds that can delay some types of cell damage and quench reactive radical species formed during oxidative reactions in metabolism. Our aim is to collect the seed of Linum usitatissimum L., screen the antibacterial activity of the solvent extracts of this plant against some selected bacteria, and identify the bioactive chemical compounds from the plant extracts using preliminary phytochemical analysis. To investigate the antioxidant activity of methanol, ethyl acetate, and chloroform extracts of Linum usitatissimum...
Read More
Posted by admin on Aug 31, 2023 in |
Objective: The study aims to evaluate the granuloma and associated inflammatory mediators inhibition potential of the chloroform extract of Piper pedicellatum C. DC. Leaves, as plant leaves, are being used traditionally in the mitigation of various inflammatory conditions; however, there is an insufficiency of scientific evidence about the relevant bioactivity of the plant. Method: The cotton pellet induced-granuloma model in rats is used in the study, providing insights into subsequent phases of inflammation. Piper pedicellatum C. DC. Leaf chloroform extract at 200 mg/kg and 400 mg/kg and indomethacin (reference standard, 10 mg/kg) were used during treatment. Results: The chloroform extract at 200 mg/kg and 400 mg/kg dose inhibited exudate formation by 25.23% and 38.43%, respectively, and inhibition of granuloma tissue was found to be 27.62% and 40.33%, respectively. Further significant decrease in serum inflammatory markers CRP and PGE2 and reduced cytokine TNF-α and IL-6 were observed in chloroform extract and indomethacin-treated rats. In contrast, a substantial increase in anti-inflammatory cytokine serum IL-10 levels was noted in treated rats. Conclusion:...
Read More
Posted by admin on Aug 31, 2023 in |
Tuberculosis (TB) is caused by a bacterium called Mycobacterium tuberculosis, identified as a global health emergency by the world health organization. The development of drug-resistance stains due to spontaneous gene mutation has been a major boost to research in the pathogenicity and biochemistry of Mtb. To combat drug resistance to tuberculosis, new drugs and methodologies are emerging. Since, starting itself mycobacterium complex cell wall has been a choice for widely selected targets for anti-TB drugs. Peptidoglycan, arabinogalactan, and mycolic acid are the basic layers supporting cell growth. The current work investigates virtual screening for optimal small molecule inhibitors targeted against selected mycolic acid targets (β-keto acyl ACP synthase, enoyl acyl ACP-reductase and Polyketide synthase 13). A small library of 485 compounds was designed and docked into a selected target core to identify the potential inhibitor. The designed compounds were subjected to docking studies using Glide (Schrodinger). InhA was the most suitable mycolic acid inhibitor target for the designed compounds. Further, the effectiveness of the study was evaluated by comparing the...
Read More
Posted by admin on Aug 31, 2023 in |
Pan-genome analysis can identify the core genome, which is the total number of genes present in all strains of a bacterial species. Further essential genes can be identified in this core set of genes which gives us a minimal number of genes required for the survival of the bacteria. This set of genes can be analyzed to develop new antimicrobial agents against pathogenic, multidrug-resistant bacteria such as Listeria monocytogenes which causes several primary and secondary infections in humans. Pan-genome analysis of 38 strains of Listeria monocytogenes was performed to estimate 2331 genes in core genome, 1056 genes in the dispensable genome, and 152 genes in the strain-specific genome. Essentiality analysis of 2331 core genome proteins predicted 965 essential core-genome families (ECGFs). Furthermore, the identification of host non-homologous proteins using BLASTP with the Homo sapiens proteome resulted in 485 non-homologous essential core-genome families (NH-ECGFs). These putative proteins can be analyzed to identify novel drug targets that could generate broad-spectrum, safe and effective therapeutic agents against Listeria monocytogenes infections in...
Read More
