Posted by admin on Aug 1, 2014 in |
A simple, accurate RP-HPLC method has been developed for simultaneous determination of Lopinavir (LPV) and Ritonavir (RTV) from tablet dosage form on reversed phase Brownlee C18 column. The sample was analyzed using acetonitrile: 10 mM ammonium acetate buffer (pH 4.5): Methanol (40:30:30) as a mobile phase at flow rate of 1.0 ml/min and detection at 210 nm using UV detector. The limit of detection (LOD) was found to be 0.061 µg /ml and 0.083 µg /ml and the limit of quantification (LOQ) was found to be 0.184 µg /ml and 0.25 µg /ml for LPV and RTV, respectively. Linearity was observed in the concentration range of 5-35 µg/ml. Both the drugs were spiked in 5% proportion with human plasma, extracted by plasma protein precipitation by using acetonitrile and analyzed by HPLC. The retention time for RTV and LPV was found to be 10.30 and 12.58 min respectively. The % recoveries of RTV and LPV were found to be 86.38 to 93.24% and 88.56 to 92.34% respectively. The LOD was...
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Posted by admin on Aug 1, 2014 in |
Snake venom is cocktail of various enzymes, toxins. Snake venom components have major role as therapeutics application in current drug industry. Snake venom toxins plays vital role as one of the major component in case of bite and most of the toxins are lethal which are of various types such as cardio-toxins, myotoxins and neurotoxins etc. The Indian Cobra neurotoxins sequences were retrieved form Swissprot Database. The ten neurotoxins were characterised and three were modelled using in silico approach whose structure were not available on Protein Data Bank and protein model portal. The neurotoxins were modelled using homology modelling approach and energy minimisation was carried out for all the three neurotoxins. The stearic hindrance was removed using chiron server. The Ramchandran plot was used to validate the modelled structure which provides idea of the modelled structure conformation and configuration and it was satisfactory. Further the modelled structures will be used to understand docking with the suitable...
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Posted by admin on Aug 1, 2014 in |
In this study, the methanol extract of Cuscuta reflexa Roxb.belonging to the family Convolvulaceae was prepared by successive extraction procedure (petroleum ether and methanol) in soxhlet apparatus. The plant was used traditionally in India, Bangladesh, China, Thailand and other Asian countries for treating various ailments. It is known to exhibit antiepileptic, antitumor, anti-inflammatory, anticancer, antibacterial, antiviral, antioxidant properties. In the present study our aim was to find out its phytoconstituents in the methanol extract of C. reflexa and also to find out its mutagenic and antimutagenic potential by Ames test against known positive mutagens 2-aminofluorine (2-AF), 4-nitro-o-phenylenediamine (NPD) and sodium azide using Salmonella typhimurium TA 98 and TA 100 bacterial strains both in the presence and absence of metabolic activator S9. The phytochemical investigation ofmethanol extract of C. reflexa Roxb. yielded three flavonoids Isorhamnetin, Isorhamnetin-3-O-glucoside and Isorhamnetin-3-O-robinobioside. The structures of the isolated flavonoids were established by detailed spectral analysis including 2D NMR spectral study. The extract exhibited significant antimutagenic activity againstNPD and sodium azide for S. typhimurium TA98 &...
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Posted by admin on Aug 1, 2014 in |
A rapid, precise, accurate, sensitive, simple, fast and reliable spectrophotometric method has been developed for determination of Fluoxetine in bulk and pharmaceutical dosage forms. The solubility of Fluoxetine was also examined in various solvents like distilled water, methanol, ethanol, acetonitrile, HCL, chloroform etc. For the determination of working wavelength different concentrations of Fluoxetine (10-60μg/ml) in water: methanol (9:1) was scanned using UV-VIS spectrophotometer within the wave length region of 200-400 nm against water and methanol mixture as blank. For the calibration curve the prepared concentrations were scanned at 216 nm in UV-VIS spectrophotometer. The R2 value was found to be 0.999. Then the standard deviation was found to be 0.0065. Then the stability study was performed against neutral, acidic and basic condition at 60°C as per ICH...
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Posted by admin on Aug 1, 2014 in |
In this research, a new series of total twenty derivatives (39-58) of titled compound were synthesized with the help of substituted acetophenone which undergoes Aldol condensation reaction with dimethyformamidedimethylacetal (DMFDMA) to form corresponding unsaturated carbonyl compounds such as enaminones. The compound 2-thioxo-1,3-dihydropyridino[2,3-d] pyrimidin-4(1H)-one were obtained by reacting enaminones with 6-amino-2-thioxo-2, 3-dihydropyrimidin-4(1H)-one in the presence of dioxane. In this reaction, 6-amino-2-thioxo-2, 3-dihydropyrimidin-4(1H)-one acts as a nucleophilic reagent. Thus, the titled compounds were obtained by cyclizing an intermediate followed by desulphuration with the liberation of hydrogen sulphide gas in the presence of acetic acid. The homogeneity and purity of synthesized compound was ascertained by physical constant determination and chromatographic methods. The structure of the synthesized derivative was further confirmed by spectral (FTIR, NMR, MS) and elemental (C, H, N) analysis. The anticonvulsant activity of the compounds (39-58) was evaluated against Maximal Electroshock (MES)-induced seizures. The compounds, those found effective, were as well tested against subcutaneous pentylenetetrazole (scPTZ)-induced seizures model in mice. Using the rotorod procedure, the neurotoxicity was assessed. The results...
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