Posted by admin on Jul 31, 2014 in |
Background: Cervical cancer (CC) is the second leading cause of cancer deaths in women, with more than 80% of these occurring in developing countries that have limited access to screening programs. Therefore there is a need to develop diagnostic and prognostic biomarkers which can be quantifiable and help clinical oncologists at the first interaction with the suspected patients. The aim of this study was to correlate the serum markers with the diagnosis of distant metastasis, disease recurrence, therapy monitoring and prognosis of the cervical cancer. Methods: The study group consisted of 50 metastatic cervical cancer patients and 50 benign cervical cancer patients in the age group of 20-80 years. Blood samples (5ml) were collected for analysis after Informed consent was obtained from each patient prior to sample collection. The markers (CEA and CA125) were analyzed by ELFA and sandwich ELISA methods respectively and compared to their reference values. Results and conclusion: Statistical analysis of the results showed that the incidence of the disease showed a direct correlation with the...
Read More
Posted by admin on Jul 31, 2014 in |
Sixteen 1-(substituted) phenyl, 1- thiocarbamoyl -3-phenyl-5-(2′′-Furyl) / Phenyl- ∆1-pyrazoline derivatives were synthesized. The chemical structures were confirmed by IR, H1 –NMR and analysis. The antidepressant activities of the compounds were investigated by Porosolt’s behavioral despair test on albino mice. 3-phenyl-5-(2′′-chlorophenyl)-4,5-dihydro-1H-pyrazole-carbothioamide (2f),1-(2,4-dinitrophenyl)-3-(3′-hydroxy-phenyl)-5-(2′′-methoxy phenyl)-4,5-di- hydro-1H-pyrazole (2k), 1-(2,4-dinitro phenyl)-3-(3′ -hydroxyl phenyl)-5-furyl – 4,5-dihydro-1H-pyrazole (2m) significantly reduced the duration of immobility times by 23.58-25.76% at 25mg kg-1 dose level using imipramine as standard reference. Anticonvulsant activities of the compounds were examined by Maximal Electroshock Seizure (MES) using Phenytoin as standard reference, and neurotoxicity were determined by Rotarod toxicity test on albino mice. 1-(2, 4-dinitrophenyl)-3-phenyl-5-(2′′chlorophenyl)-4,5-dihydro-1H-pyrazole(2e),1-(2,4-dinitro phenyl) -3-(3′ -nitro phenyl)-5-furyl- 4, 5- dihydro-1H-pyrazole(2o), and 3-(3′ -nitro phenyl)-5-furyl-4,5-dihydro-1H-pyrazole–thiocarbamide (2p) had good protection against the Maximal Electroshock Seizure (M.E.S) at 20 mg kg-1 dose levels. These compounds (2f 2k, 2m, 2e, 2o, 2p) did not show any neurotoxicity in the Rotarod test at 20mg kg-1 dose...
Read More
Posted by admin on Jul 31, 2014 in |
The synthesis, characterization and spectroscopic studies of new N-substituted pthalimide analogues 2–7 with analgesic activity were described. The compounds were synthesized using phthalic anhydride with various appropriate amines (Triazolamine, Benzocaine, p-Nitro-aniline, pyrazinamide, Phenazone and glycine) in reflux synthesizer. The purity of the compounds was determined by TLC. With the physical and spectral data the structure of the new synthesized compounds were elucidated. The newly synthesized compounds were subjected for the screening of CNS activity by using standard experimental models. The analgesic activity of the selected compounds 2-7 were evaluated in vivo by ip carboxymethylcellulose (CMC) and acetic acid- induced ‘writhing’ test in mice. Compounds 2-7 were exhibited significant analgesic activity in hot-plate and acetic acid inducer screening test comparable to the control CMC (Carboxymethylcellulose) and...
Read More
Posted by admin on Jul 31, 2014 in |
The present study has been designed to investigate the effect of modulation of tumour necrosis α (TNF-α) on naloxone induced opioid withdrawal syndrome. Chronic morphine (5mg/kg,i.p) administration followed by a single injection of naloxone (8mg/kg, i.p) was used to precipitate opioid withdrawal syndrome in mice. Behavioral observations were made immediately after naloxone treatment. Withdrawal syndrome was quantitatively assessed in terms of withdrawal severity score and frequency of jumping, rearing, fore paw licking and circling. Administration of pirfenidone (200mg/kg, p.o) markedly and dose dependently attenuated naloxone induced morphine withdrawal syndrome. Thus, it is proposed that glial cells activation via toll like receptor (TLR) linked mechanism might be involved in the development of opioid dependence and precipitation of its withdrawal...
Read More
Posted by admin on Jul 31, 2014 in |
Turmeric, curcuma longa L of Zingiberaceae family is a widely cultivated spice in India and other Asian countries. Turmeric is rich in curcuminoids, and recognized for their broad spectrum of biological activities, curcuminoids vary in chemical structures, physico-chemical characteristics as well as the functional properties. This study focused on separation of individual curcuminoids from spent turmeric oleoresin considered as an industrial waste and its purification by column chromatography followed by purity analysis by HPLC. Chromatographic purification of spent turmeric oleoresin was done with Diaion HP 20 resin, fractions were collected. Curcuminoid enriched oleoresin fractions combined & then subjected to hexane precipitation, purified curcuminoid enriched fraction obtained. Purified curcuminoid enriched fraction subjected to silica gel column chromatography to separate the individual curcuminoids with chloroform: methanol at increasing polarity. 10ml each 40 fractions were collected and TLC profile done. Same Rf fractions collected & evaporated. Total curcuminoids of individual fractions were determined by HPLC. Recrystallization of each compound was done using methanol: chloroform (5:1) at 3°C. Curcumin, Demethoxy curcumin, Bisdemethoxy curcumin...
Read More
