Posted by admin on May 1, 2014 in |
Anda leghyam (AL) a herbo-animal Siddha formulation is administered for Gunmam (Acid peptic diseases) by many Siddha physicians popularly. Anti ulcerogenic activity of the AL has been studied using Aspirin-Pylorus ligation induced gastric ulcer methods (four groups/method, n=6, Aspirin 400mg/kg, Omeprazole 10mg/kg, AL 250,500mg/kg) in wistar albino rat models. Animals were sacrificed and their stomachs were subjected to macroscopic and microscopic ulcer index findings. Statistical data were analyzed by one way ANOVA followed by student’s t-test. This study concluded that AL had significant anti-ulcer effects in experimental animals with ulcer induced by aspirin and pylorus ligation; it showed a dose dependent protection against aspirin (400 mg/kg body weight) induced ulcers in rats and it produced a significant reduction of ulcer index in the dose of 500mg/kg bodyweight. AL showed a statistically significant P value < 0.05 and <0.01 at dose level 250 mg/kg and 500 mg/kg respectively as compared to control. Acute toxicity study was carried out as per OECD guidelines and the LD50 was found to be greater...
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Posted by admin on May 1, 2014 in |
A simple and cost effective spectrophotometric method is described for the determination of Meropenem (MP) in pure and pharmaceutical formulations. The method is based on the formation of dark yellow colored chromogen when the drug reacts with 1, 2 naphtho quinone -4-sulphonic acid sodium salt (NQS) reagent in alkalline medium. The method involves the addition of excess NQS of known concentration in the presence of 1.5 mL NaOH and the unreacted NQS is determined by the measurement of the λmax 449 nm, which was found to be the most suitable of several tests. This method was applied for the determination of drug contents in pharmaceutical formulations and enabled the determination of the drug in microgram quantities (0.5 to 3.0 mL). No interference is observed from excipients and the validity of the method was tested against reference method. The colored species has an absorption maximum at 449 nm for MP (Method A) and obeys beer’s law in the concentration range 0.02 – 0.12 mg/mL of MRP. The apparent molar absorptivity...
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Posted by admin on May 1, 2014 in |
The present study deals with the in vitro anti-inflammatory and anti-arthritic activity in methanolic extracts of in vivo (leaf and stem) and in vitro (callus) plant parts of Cocculus hirsutus. The previous phytochemical analysis of methanolic extract of Cocculus hirsutus has indicated the presence of several physiologically active phytochemicals such as phenols, flavonoids, triterpenoids, steroids, alkaloids etc. Since these compounds are of pharmacological interest, coupled with the use of this plant in traditional medicine, prompted us to check all in vivo and in vitro plant parts of Cocculus hirsutus for in vitro anti-inflammatory activity by HRBC (Human Red Blood Cell) membrane stabilization method and anti-arthritic activity by the inhibition of protein denaturation method. The methanolic extracts of all plant parts exhibited notable anti-inflammatory activity and remarkable anti-arthritic action. The membrane stabilization was found to be maximum in leaves (88.8% at a dose of 1000µg/ml) and that of protein denaturation was also found to be maximum in leaves (65.85% at a dose of 1000µg/ml) as compare to other in vivo...
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Posted by admin on May 1, 2014 in |
Flavoxate Hydrochloride is an antispasmodic, mainly used for treating painful urination, urgency of urination and incontinence. The primary objective of the study was to formulate extended release capsules of Flavoxate hydrochloride to reduce dosing frequency and decreasing the associated side effects. The extended release capsules were formulated using extrusion spheronisation process with ethyl cellulose, HPMC polymers. First, pellets (C1 to C6) containing varying amounts of Ethyl cellulose or Microcrystalline cellulose, Dicalcium phosphate and Eudragit NE30D55 were prepared using extrusion spheronisation (C6 was selected). Then the selected pellets were coated with different drug loading solution (DL 1 to DL7) with varying concentrations of Methocel (DL7 was selected).Finally extended release coating was applied to optimized drug loaded pellets and ten batches (E1 to E10) were prepared.. The dissolution profile comparison of the prepared batches E1 to E10and market preparation (Urispass) was done by difference factor (f1) and similarity factor (f2) determination. The formulation E9 (EC: HPMC 1:1 ratio) with a difference factor f1 (5.9) and similarity factor (f2) of 64.6 was...
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Posted by admin on May 1, 2014 in |
BCR-ABL Tyrosine Kinase inhibitor is an ideal pharmacological target for Chronic Myeloid Leukemia (CML). After the effective use of Imatinib as a therapeutic agent for Chronic Myeloid Leukemia, resistance has been observed in much patience with the treatment of Imatinib. The main objective of this study is to develop some new novel BCR-ABL Tyrosine Kinase inhibitor for the treatment of Chronic Myeloid Leukemia by Structure Activity Relationship (SAR) and Pharmacophore based drug design approach with Imatinib as a prototype drug. Moreover to overcome the resistance with Imatinib and get some new innovative BCR-ABL Tyrosine Kinase inhibitor with increased potency. In this study, we have designed some new BCR-ABL Tyrosine Kinase inhibitor and reported them as potentially new BCR-ABL Tyrosine Kinase inhibitor by using molecular docking analysis and various free internet based Insilco tools. The drug properties like toxicity, metabolic site, Binding energy, Inhibition constant, Ligand efficiency and many other parameters are predicted through In-silico...
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