Posted by admin on Sep 1, 2014 in |
In the present study, the effort has been made to formulate the effervescent bioadhesive vaginal tablet of Metronidazole for localized treatment of Bacterial vaginosis. Among various bioadhesive polymers carbopol 934P, hydroxyl propyl methylcellulose K4M, methylcellulose, and sodium carboxymethyl cellulose were selected. Eight different formulations were made using a single polymer as well as a combination of two polymers in 1:1 ratio by direct compression technique. Formulations containing carbopol 934P alone (F4), carbopol 934P and sodium CMC in 1:1 ratio (F8) and carbopol 934P and hydroxypropyl methylcellulose K4Min 1:1ratio (F6) showed high, medium and low swelling behavior and bioadhesion property respectively and other formulations were found to disintegrate in 1 h time during swelling study as well as during dissolution study. In-vitro drug release was found to be 51.03% for F4, 42.98% for F8, and 34.87% for F6 at 12 h. F4 was found to release half of the total drug in 12 h. Based on swelling behavior, bioadhesion test, and in-vitro drug release, F4 was selected as the best...
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Posted by admin on Sep 1, 2014 in |
The objective of the present study is to develop and validate a method for the estimation of gatifloxacin (GTX) in the tablet dosage form. An isocratic reversed-phase high-performance liquid chromatographic (HPLC) method was developed to estimate gatifloxacin using X terra C18 column (4.6 mm ´ 150 mm I.D., 5µm particle size) and the mobile phase containing phosphate buffer (pH adjusted to 3.5 with dilute phosphoric acid) and acetonitrile (60:40 v/v) at the flow rate of 0.5 ml/min. The UV detection was carried out at 298 nm. The retention time of gatifloxacin was found to be 2.340 min. The developed method was validated concerning accuracy, precession, linearity, selectivity, ruggedness, and robustness. Linearity for gatifloxacin was observed in the concentration range 0.01 to 0.50 µg/ml. Percent recovery was found to be 99.67. The results confirmed that the proposed method is specific, rapid, reproducible, and suitable for the routine determination of...
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Posted by admin on Sep 1, 2014 in |
Swertia chirayita (SC) has been traditionally used for the treatment of several metabolic disorders related to health problems. We have investigated the antidyslipidemic and antioxidant potential of Swertia chirayita (SC) plant, a potent antidiabetic, and its isolated markers. The LC-ESI-MS standardized hot water extracts of different batches from the whole plant of Swertia chirayita, were tested in-vivo for their antidyslipidemic activity in Triton (WR-1339) and high-fat diet (HFD) induced dyslipidemic Charles Foster rats and in vitro for their antioxidant activity. Further solvent fractionation and isolation resulted in the identification of antidyslipidemic Mangiferin (MG) and Amarogentin (AM). Treatment of hyperlipidemic rats with MG and AM at 100 and 200mg/kg doses caused reduction in plasma levels of Low density lipoprotein (LDL) 24, 26% and very low – density lipoprotein (VLDL) 19, 25% as well as increase in levels of High density lipoprotein (HDL) 18, 22% respectively. In rats with HFD, MG and AM significantly reduced levels of VLDL lipids (26% and 27%) as well as Low-density lipoprotein-Total cholesterol (LDL-TC) 27% and...
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Posted by admin on Aug 31, 2014 in |
The present study was designed to evaluate the oculohypotensive effect of enalaprilat in comparison to timolol using an intraocular pressure (IOP) recovery model. The study was carried out in 18 adult albino rabbits of 2.5-3.0 kg which were divided into 3 groups.Group Ireceivednormal saline,group II received enalaprilat (0.1%) and timolol (0.5%) was administered in group III. IOP recovery model was used to assess the effect of drugs on IOP recovery time. Additionally relative percent of IOP (IOPt%) at various time intervals was calculated by equation: IOPt% = (IOPt / IOP-40) x 100 % . Condition of conjunctiva was also examined. There was a significant (p= 0.006) delay of the IOP recovery with both timolol (150 ± 8.65 minutes) and enalaprilat (130 ± 6.83 minutes) when compared to normal saline (110 ± 6.83 minutes). There was also a change in relative percent of IOP at all the time intervals being significant at 80 minutes (p=0.036), 100 (p=0.006) and at 120 minutes (p=0.032). There was no congestion of conjunctiva with both...
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Posted by admin on Aug 1, 2014 in |
Piperine, a major alkaloid in black pepper is one of the most promising bioenhancers till date. Other methods used for its extraction suffer drawbacks such as poor extraction efficiency, tedious and expensive isolation methodology, piperine photodegradation, etc. Hence a simple, rapid and efficient method has been developed for the isolation of piperine from the fruits of Piper nigrum. The methods under study involve extraction of piperine with various solvents such as ethanol, glacial acetic acid and dichloromethane. Then isolation and purification were followed by separate classical methods for respective extracts. Compared to other two methods, the novel method using glacial acetic acid proved to be effective in isolating piperine with higher yield and in higher purity. Hence extract derived using glacial acetic acid was further subjected to column chromatography. Finally raisins removal and crystal formation were facilitated by recrystallization with solvent ether after alkali wash. Then identification of the compound was confirmed by various analytical methods TLC, melting point, UV-visible spectrophotometer, FT-IR, HPLC and compared it with authentic piperine...
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