Posted by admin on May 1, 2014 in |
Hypoglycaemic and anti-hyperglycaemic effects of Portulaca oleracea Linn. (POL) was evaluated in experimental rat models in vivo. The aqueous extract of POL was subjected to preliminary toxicity screening by an acute toxicity study in Wister albino rats. The POL was found to be safe in the doses used and there was no mortality up to a dose of 3000mg/kg orally after 24 hours. Hypoglycaemic effects of POL extract with doses 200mg, 400mg and 800mg were evaluated on Wistar albino rats by using a standard protocol. Anti-hyperglycaemic effect of POL extract with the same doses i.e. 200mg, 400mg and 800 mg were also studied in alloxan induced diabetic albino rats. The aqueous POL extract at doses 800mg showed significant hypoglycaemic effects as compared with a standard drug like glibenclamide. The same doses of POL also showed anti-hyperglycaemic properties in alloxan induced diabetic rats. The present study showed that the aqueous POL extract has hypoglycaemic as well as anti-hyperglycaemic...
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Posted by admin on May 1, 2014 in |
The aim of the present study is to develop a nanoparticle formulation as a potential carrier for topical drug delivery. Colchicine loaded Nanoparticles (Colchicine-NPs) were prepared by the double emulsion solvent evaporation method using biodegradable and non biodegradable polymers alone or as binary mixtures. Based on the evaluation of the entrapment efficiency, the particle size and the % drug released over 24 hours, a polymer mixture of poly-D,L-lactic acid (PLA), and Eudragit RL, in the weight ratio 1:1 was selected for further studies. The following formulation variables were optimized: the pH of the external aqueous phase was adjusted to a value of pH 6 in order to decrease the solubility of colchicines; the solvent composed of dichloromethane and acetone 1:1 (v/v) was selected to obtain maximum water miscibility; CaCl2 was selected as a counter ion additive in the external aqueous phase; Span was used in the internal organic phase to increase entrapment efficiency and medium chain triglyceride was found to be most efficient at a concentration of 30% w/w....
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Posted by admin on May 1, 2014 in |
As Antioxidant potential of many plants is mainly due to phenolic components such as phenolic acid, phenolic diterpense therefore total phenolic content and antioxidant potential of cucurbita maxima. (Pumpkin) powder was determined respectively by using Folin-Ciocalteu reagent and diphenyl-picryl-hydrazyl (DPPH) assay. In this method the antioxidants present in the plant extracts reacted with DPPH, which is a stable free radical and converted it to 1,1-diphenyl-1,2-picryl, hydrazine. Total phenolic content of pumpkin was determined using Folin-Ciocalteu reagent and gallic acid as standard, amount of phenolic compound was found to be 6.5 mg/gm powder of the drug. The high fiber content of pumpkin helps to aid proper digestion and in traditional medicine it is used as an anthelmintic, taenicide, diuretics and in the treatment of hemorrhoids so we compared the anti oxidant potential of pumpkin powder with marketed preparation (Pilex tablet). The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a compound in inhibiting biological or biochemical function. IC50 value of pilex tablet = 8.53 µg/ml & IC50 value...
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Posted by admin on May 1, 2014 in |
Phytochemical screening of thirteen medicinal plants used in the treatment of sexually transmitted infections (STI), HIV/AIDS in Benin, showed that mucilage, gallic tannins and anthocyanins were the most common chemical groups. Water-ethanol (HE): 4/6 v/v, aqueous (A) and dichloromethane (DCM) extracts were prepared for each plant and tested for toxicity against shrimp larvae (Artemia salina Leach). Twenty two extracts were found not toxic (LC50 ≥ 0.1 mg / mL) with A13 aqueous extract of Vitex doniana Sweet (LC 50 = 1.35 mg / mL) as the most selective one. Antibacterial tests of extracts showed bacteriostatic activity on both strains tested (E. coli ATCC 25922 and S. aureus ATCC 25923) with the minimum inhibitory concentrations (MIC) ranging from 0.625 mg / mL to 5 mg/mL on E. coli and 0.078 to 1.25 mg/ml on S. aureus. Only the hydroethanolic extract of Acanthospermum hispidum (HE7) showed bactericidal activity against both strains with minimum bactericidal concentrations (MBC) values of 2.5 mg/mL and 0.625 mg/mL on E. coli ATCC 25922 and S. aureus...
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Posted by admin on May 1, 2014 in |
A fast simple sensitive precise, accurate and reproducible RP-HPLC method was developed and validated for the analysis of rivastigmine hydrogen tartrate in transdermal drug delivery system. Rivastigmine tartrate is soluble in water so it was used as solvent. The separation was conducted by using C-18 RP-HPLC column which was maintained at ambient temperature.The mobile phase consist 0.01M ammonium acetate buffer and acetonitrile (70:30 v/v) was delivered at a rate of 1ml/min. The analysis was detected by using UV detector at the wavelength 219nm. The method is validated for its accuracy precision,ruggedness, linearity and range. The method was found to be linear over the concentration range 50-100 µg/ml (r2 = 0.999). The retention time for rivastigmine was found to be 4.40min.The total run time of chromatogram was about 10min.The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. The above method was a rapid and cost-effective quality-control tool for routine analysis of Rivastigmine hydrogen...
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