Posted by admin on Jul 1, 2014 in |
The aim of the present study was to develop suitable controlled release injectable vancomycin thermoreversible chitosan/ β-glycerophosphate hydrogel system for treatment of orthopedic infections. The effect of different formulation variables on the gelation temperature was studied. The prepared vancomycin loaded Chitosan/β-glycerophosphate thermoreversible hydrogels were characterized regarding gelation temperature, in-vitro vancomycin release and enzymatic degradation rate in presence of lysozyme. Antimicrobial activity of the released vancomycin was tested against the gram-positive methicillin resistant Staphylococcus aureus (MRSA) by agar diffusion method. The results showed that chitosan/ β-glycerophosphate solution were able to form gels at body temperature depending on chitosan degree of deacetylation, concentration, pH and β-GP concentration. The prepared thermoreversible hydrogels sustained vancomycin release for 21 days. Released vancomycin concentrations ranged from 3 to 95 folds the reported minimal inhibition concentration (MIC 90) for MRSA at all release study time points. Chitosan/β-glycerophosphate thermoreversible hydrogels were enzymatically degradable and 17.57% of the gel weight remained after 21 days. The preserved antimicrobial activity found after release of the antibiotic from the hydrogel elected...
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Posted by admin on Jul 1, 2014 in |
Alizarin [1, 2 di hydroxy anthraquinone] is the major pigment, extracted from Rubia Cordifolia L. The aim of the present work is to extract manjith dye and alizarin pigment from Rubia Cordifolia L., and use it in the film coating of triphala guggle ayurvedic (TGA) tablet. The optimum extraction conditions for manjistha dye are 600C and 60 minute stirring with methanol (80%) and M:L ratio(1:10) with particle size 0.50mm. Separation and purification of alizarin was carried out by column chromatography. The purified alizarin was characterized by UV-Vis, and FT-IR, techniques. Acetylation of alizarin has been carried out with acetic acid in presence of sulphuric acid. Coating offers many benefits namely, improving asthetic qualities of dosage forms, masking unpleasant odour or taste, easy ingestion, and improving product stability. In the present study core tablets of triphla guggul are coated with manjistha extract, manjistha dye, pure alizarin, and acetylated alizarin. After coating, the stability of tablets were evaluated at regular intervals. The purpose of the stability testing is to provide proof...
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Posted by admin on Jul 1, 2014 in |
Trichosanthes cucumirina L., locally known as “Chachindra” among the tribal and rural mass of Similipal Biosphere Reserve (SBR) forest. Plant parts are used as ethnomedicine among inhabitants of SBR. They use the fruits, leaves and roots against various diseases and traditional practices are continuing since long. Leaves are used against microbial infections, roots against diabetic and fruits against killing of stomach worm. Young fruits are used as vegetables as well as medicine. Keeping this traditional practice in view, assessment of food values and antibacterial properties were done. Fruits are rich with carbohydrate (26.24 %), lipid (2.20 %), protein (1.50 %), fiber (1.96 %) and good amount of moisture. Plant parts showed diverse bioactive compounds such as tannin, saponin, and flavonoid present in most of extracts of leaves, fruits and roots of T. cucumirina L. The antibacterial activity of plant extracts showed excellent against MTCC 1252, MTCC 1457, MTCC 3906, MTCC 1926, MTCC *497. Presents study highlights the nutritional, ethnobotanical and antibacterial activity of the plant and emphasis upon the...
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Posted by admin on Jul 1, 2014 in |
To evaluate the protective effect of fenugreek (Trigonella foenum graecum L.) on alloxan induced diabetic mice, 15 male albino mice weighing 26-30 gm were used. They were divided into 3 groups. Control group received subcutaneous injection of 0.15 M acetate buffer for 15 days. Alloxan induced group received subcutaneous injection of alloxan 150 mg/ kg body weight to induce diabetes. Recovery group received subcutaneous injection of fenugreek seed extract 15 mg/kg body weight per day for 15 days. At the end of experiment histological and ultrathin sections of pancreas prepared. In diabetic mice disturbances in pancreatic arrangement is observed. While in electron microscopic study pyknotic nuclei, vacuolation and morphological changes in mitochondria, endoplasm reticulum and degranulation of β cells were observed. After treatment with fenugreek repair in pancreatic arrangement especially in β cells was clearly observed. In conclusion fenugreek seed extract could normalize the diabetes and provides protection to pancreatic tissues from damaging effect of...
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Posted by admin on Jul 1, 2014 in |
Chronic Kidney diseases are increasingly recognized as a problem worldwide. Incidence of chronic kidney diseases is doubled in last fifteen years. The kidney is both cause and victim of hypertension. Hypertension is a serious risk factor for chronic kidney disease. The purpose of this study was to measure serum creatinine level in hypertensive patients as an indicator of kidney functioning and compared it with normal healthy controls. This study was carried out in Shanti Prapann Sharma Government Hospital, Rishikesh. In present study, the volunteers were selected from General Medicine department of Government hospital Rishikesh from May 2009 to May 2010. A total of 120 volunteers were recruited for this study. Out of them 70 were hypertensive subjects (38 males and 32 females) and 50 were normotensive subjects (27 males and 23 females). Hypertension was defined as per the recommendations of JNC7th Report. After 12 hours fast the blood samples were collected with the help of trained laboratory technician from all individuals without anticoagulant and centrifuged at 3000 rpm for...
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