Posted by admin on Apr 1, 2014 in |
A rapid and sensitive method for the determination of the best conditions that allow us to calibrate the ibuprofen in drug is presented. This approach uses high performance liquid chromatography with DAD detector, which is a separation method most used because it is fast, easy, and maintains the nature of the mixture to be separated. The spectra of ibuprofen were recorded using several mobile phases “58%Na2HPO4 (50mmol/l), 42%ACN” and “60%ACN, 40% water “when the pH values are (pH=2.5 and pH=5); the flow rate values are 0.9ml/min -1.5 ml/min with detection wavelength of 240nm; and two stationary phases using the C18 column and Phenyl urea column (25cm x 4.6mm, 0.5µm). The ibuprofen sample: 1 mg of ibuprofen dissolved in 1 mL of water. For this we have tried through our study aiming relying on foreign...
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Posted by admin on Apr 1, 2014 in |
Zopiclone (ZPC), a cyclopyrrolone derivative, is a short-acting hypnotic agent. Its pharmacological properties include hypnotic, sedative, anxiolytic, anticonvulsant and muscle-relaxant actions. ZPC has a single chiral centre, and its enantiomers are S-zopiclone (S-ZPC) and R-zopiclone (R-ZPC). S-ZPC was adjudge to possess two- fold enhanced pharmacological activity than racemate (ZPC) while its opposite enantiomer R-ZPC appears to have almost zero activity and elevated toxicity. A high performance LC-MS\MS method employing positive electrospray ionization was developed for the simultaneous determination of both S-ZPC and R-ZPC in human plasma samples by using S-Zopiclone d8 as internal standard. Analytes were extracted from plasma samples by employing solid phase extraction technique. After extraction, 5µL of clean sample was injected on to chromatographic system and distinct separation achieved on chiralpak IC-3, 0.46 cm I.D*15 cm L column with the isocratic mobile phase consisting of a mixture of Ammonia in Acetonitrile and Milli Q Water. Detection was performed using API 4000 MS that monitors specific transition per compound in Multi Reaction monitoring mode. Limit of quantitation...
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Posted by admin on Apr 1, 2014 in |
AIDS, a pandemic disease caused by HIV has a high mortality rate. Therapeutic failures have risen due to the emergence of resistant viral strains and adverse side-effects of Anti-HIV drugs. Thus, there arises a need to develop novel and promising anti-HIV drugs. BMS-378806 (BMS-806) inhibits the initial stages of HIV-1 infection by blocking the binding of viral gp120 protein to host-cell CD4 receptor. Protein- Ligand interaction plays a major role in structure-based drug designing. In this study, analogs of BMS-378806 drug for HIV target protein gp120 were virtually designed using Marvin Sketch 4.1.10 and molecular docking studies were carried out using HEX 4.1 docking software. From the results, it was observed that, analog-6 showed better binding affinity to gp120 with Etotal = -102.67 than the original BMS-378806 drug, thus indicating that this compound might be considered as a potential drug candidate against gp120 for controlling HIV-1 infection. Further studies need to be carried out to evaluate the pharmacological efficacy of these...
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Posted by admin on Apr 1, 2014 in |
Niosomes, an emerging class of novel vesicular systems, are non-ionic surfactant vesicles which can entrap both hydrophilic and lipophilic drugs. Niosomes are incorporated into in situ gels for sustained release of drug and to prolong the residence time. The aim of present study is to formulate and evaluate ocular niosomal in situ gels of Linezolid. Linezolid is a potent synthetic oxazolidinone derivative active against a broad range of gram positive and gram-negative aerobic and anaerobic bacteria. Niosomes were prepared using various surfactants (span 20, span 40, span 60 and span 80) in different ratios using thin film hydration technique. They were evaluated for particle size, entrapment efficiency and in vitro drug release. Niosomes prepared using cholesterol and span 60 in the ratio 1:2 showed higher entrapment efficiency and better in vitro drug release. The optimized formulation was formulated as in situ gels using Carbopol 971P and HPMC K4M in different ratios and evaluated for gelling capacity, pH, viscosity, in vitro drug release, drug content, antimicrobial activity and ocular irritation...
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Posted by admin on Apr 1, 2014 in |
Cutaneous leishmaniasis (CL) is a public health problem in Syria call for this parasite’s epidemiological and evolutionary investigation. Evaluation of its population structure by multilocus enzyme electrophoresis (MLEE), current gold standard for classification, has some drawbacks ,such as the lack of discriminatory power below species level, it is labour intensive and time-consuming. In this study , MLEE has been replaced by applying PCR-RFLP of the internal transcribed spacer 1 (ITS1) for identification of leishmania at species level, on 37 samples were obtained from patients with suspected CL referred to Damascus Dermatology Hospital, 19 of them from Damascus and 18 from Aleppo. Intraspecific variation could be detected by the use of 9 hypervariable microsatellite markers. Our results showed that all samples were L. tropica. 26 genotype were detected, of wich 20 were unique to individual strains and 6 were shared by more than sample. Results also showed that there was amoderate genetic differentiation and a clear genetic flow between Aleppo and Damascus. In conclusion, in the present study, the first...
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