Posted by admin on Jun 1, 2014 in |
A study on the effect of methanol extract of red beetroot (MEBV) against CCl4 induced hepatotoxicity in male albino rats of Wistar strain (150 to 180g) was done. Five groups with six rats each were used as positive control, negative control and experimental with an oral dose of CCl4 throughintra-peritoneal (IP) route and MEBV at 100µgm/ml, 200µgm/ml & 300µgm/ml per os, respectively, for 14 days. After 14 days, animals were sacrificed; blood samples were obtained and analyzed. The results showed that CCl4 administration was associated with triple fold increases in the activities of alanine amino transferase (ALT), aspartate amino transferase (AST) and Bilirubin (P<0.05) compared with the respective mean control values. The enzymatic and non-enzymatic antioxidants such as, serum catalase, GSH, Vit. E, Vit.C, & SOD, were lower than the respective mean values of the normal control. The blood parameters have got reduced. The hapatochords and hepatocytes architecture was also observed to be disturbed. However, treatment with MEBV restored the enzyme activities of the liver ALT, AST, Bilirubin, WBC,...
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Posted by admin on Jun 1, 2014 in |
Antioxidants can protect the human body from free radicals and ROS effects. They retard the progress of many chronic diseases as well as lipid peroxidation. The commercially used synthetic antioxidants BHA and BHT have been suspected of being responsible for liver damage and carcinogenesis. Hence, a need for identifying alternative natural and safe sources of antioxidants has been created, and the search for natural antioxidants, especially of marine origin, has notably increased in recent years. The aim of this current study is to find the antioxidant effect of marine invertebrates of molluscan group comprising gastropods, bivalves and echinoderms methanolic extract using different in-vitro assays such as DPPH, reducing power and total antioxidant activity. The present findings imply that, bivalves possess higher antioxidant activity that formed as regular seafood for mankind than other groups investigated; hence, these methanolic extracts can be served as ironic agents of antioxidants and also used as pharmaceutical agents to prevent various degenerative diseases where it demands further research towards purification and mechanisms of...
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Posted by admin on Jun 1, 2014 in |
Chicken anemia virus (CAV) encodes the protein VP3 also named “apoptin” that specifically induces apoptosis in human tumor cells. In this study, the VP3 gene cloned in p VAX1 plasmid vector was transfected in HeLa cells. The expression of CAV VP3 gene was detected using immunoperoxidase test and indirect fluorescent antibody technique. High level of gene expression was observed in HeLa cell culture. Successful expression of recombinant plasmid in mammalian cell lines shows that the plasmid could be further evaluated in field trials and hence useful tool for study of antitumor...
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Posted by admin on Jun 1, 2014 in |
Background: Neuropathic pain is a type of chronic pain that occurs when nerves in the central nervous system become injured or damaged. Currently only a limited number of topical treatments are available to treat it. Hence an attempt was made to alleviate symptoms of neuropathic pain by topical application of a local anaesthetic drug. Results: Microemulsions were formulated using Oleic acid as the oily phase and Tween 80 and ethanol in 1:1 ratio as the surfactant and cosurfactant respectively. The microemulsions thus formed were clear, transparent and aesthetic in appearance and showed low viscosity and near neutral pH. They exhibited electrical conductivity indicating they were o/w microemulsions. The particle size was in the submicron range while the polydispersity index was 0.4 indicating a uniform particle size distribution. A satisfactory and elegant gel was obtained using Xanthan gum as the gelling agent. In-vitro and ex-vivo drug diffusion indicated a sustained release of the drug over 8 hours. Anti-nociceptive activity was tested using hot plate and tail flick tests where the...
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Posted by admin on Jun 1, 2014 in |
The aim of this research work was to develop and characterise bilayer film and to investigate its potential as slow-release wound healing vehicle. The bilayer is composed of an upper layer impregnated with Diclofenac diethyl amine and a drug-free lower layer, which acted as a rate-controlling membrane. Solvent casting technique was employed to prepare transdermal films. Pre formulation studies are performed to determine solubility, melting point, compatibility and partition coefficient. Alginate based bilayer film for wound healing was successfully developed. The in vitro drug release study implied that by using the bilayer films which retain the drug for a longer period of time thus minimise the dressing changing frequency. The method adopted in this research work is genuine can be used as routine analysis of the...
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