Posted by admin on Jun 1, 2014 in |
Solvent evaporation technique is used for the preparation of Polylactic acid microspheres loaded with two antibiotics, Doxycycline and Tetracycline, by introducing different masses for each drug. They are characterized by calculation of drug encapsulation and drug loading, measuring the particle size, examining microspheres morphology, and performing FT-IR, stability and in vitro drug release studies. Moderate drug encapsulation values (38% and 24%) are observed for Doxycycline and Tetracycline respectively. Microspheres of both drugs show homogeneous size distribution varying between 80 and 110 µm. the FT-IR study has helped in verifying that both Doxycycline and the polymer are intact after microencapsulation. In the case of Tetracycline, it hasn’t revealed any significant results due to the low loading values. After 6 months of storage at different conditions, no appreciable difference is observed concerning the degradation of microspheres. In vitro release of drugs from encapsulated Polylactic acid microspheres shows that Tetracycline release is slightly faster and reaches 98% in less than 5 hours, in comparison with Doxycycline which attains the same percentage after...
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Posted by admin on Jun 1, 2014 in |
Rhizophora mucronata Lam. belongs to mangrove plants and antidiabetic activity is one of the traditional uses of R. mucronata. One of the anti-diabetic therapeutic strategies is inhibition of carbohydrate digesting enzymes such as alpha-amylase and alpha-glucosidase. Hence in the present study, 2 extract namely, aqueous and ethanol of Rhizophora mucronata Lam. were evaluated for their effect on alpha-amylase and alpha-glucosidase enzymes using in vitro assays. Ethanol extract showed the highest alpha-glucosidase inhibitory activity (IC50= 9.45 µg/ml) than aqueous extract. Ethanol extract inhibited the enzyme more potently than the standard acarbose. Further, the antioxidant activity of extracts was monitored using DPPH assay, whereas, phenolic content was estimated using Folin-Ciocalteu reagent. The alpha-glucosidase inhibitory activity of R. mucronata correlated to antioxidant activity and phenolic content of extracts. However, these extracts showed no alpha-amylase inhibitory effect. Besides, R. mucronata has shown anti-HIV potential in our previous study. Oxidative stress and anti-HIV drugs are associated with diabetes. Our current and previous studies thus report anti-diabetic, antioxidant and anti-HIV activities of R....
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Posted by admin on Jun 1, 2014 in |
Herbal drugs are emerging as a leading area for new drug discovery and development, and are being looked at seriously for health problem. Plants, especially used in Ayurvedic can provide biologically active molecules and lead structures for the development of modified derivatives with enhanced activity and or reduced toxicity. Exposure of toxicants manifests variety of pathological lesions in various organs depending upon its dose and the period of treatment. Tridax procumbens has been found to possess various significant medicinal properties. Present study is therefore done to study the histological changes on Kidney of albino rats due to effect of ethanolic extract of Tridax procumbens; orally at a single and daily administration of doses at 300mg/kg b.wt and 600mg/kg b.wt for intervals of 7, 14 and 21days. It is quite obvious that its administration may also affect the cellular organization of vital organs. After treatment period there was alterations occurred in histoarchitecture of kidney and it showed remarkable changes in cortex and medullary...
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Posted by admin on Jun 1, 2014 in |
Objective: To analyse and compare pattern of pre anaesthetic medication use in various surgical specialties. Methodology: Data was collected retrospectively from inpatient records of major surgeries in the Department of Surgery, Orthopaedics, Neurosurgery, Paediatric Surgery, Obstetrics and Gynaecology, Plastic surgery, Urology and Vascular Surgery at M.S. Ramaiah Hospitals, during the period of January 2012 to June 2012. Descriptive analysis was used to analyse the data .SPSS version 19 was used for analysis of data. Results: 752 pre anaesthetic records were analysed, the most frequently used pre anaesthetic medications, in the order of frequency among antianxiety drug is alprazolam (15.6%),followed by midazolam (7.6%),among opioid analgesics fentanyl (5.9%) followed by sufentanyl (0.1%),among antiemetic drugs ondansetron (57.3%),followed by ramosetron (22.8%) and metoclopromide (18.8%) , among gastro protective pantoprazole (74.6%),followed by ranitidine (11.9%), rabeprazole (8.3%),esomeprazole (2.9%) and glycopyrrolate was the only anticholinergic used . Conclusion: Our study gives an insight into the current practice pattern of anaesthetists for pre medication in various surgical specialties in a tertiary care hospital. . It also points...
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Posted by admin on Jun 1, 2014 in |
Street foods are very common sources of infectious diseases in Bangladesh. In present study the microbiological quality of three types of ice-creams obtained from street vendors in Dhaka city was evaluated. The microbiological quality of commercially produced Ice-creams (milk based, lolly and kulfi) was identified by examining total viable count (TVC) and association of enteropathogenic bacteria. The TVC and coliform count were determined respectively on Nutrient and MacConkey Agar after 24 hours incubation at 30̊C. Association of Salmonella, Shigella were detected on SS Agar and Vibrio on Thio-sulfate Citrate Bile Salts (TCBS) media .The average TVC for milk based, lolly and kulfi were 2.53×105 cfu/ml, 7.16×103 cfu/ml and 1.26×107 cfu/ml respectively. A total number of 86 strains were isolated from 3 types of Ice-creams. The association of pathogenic bacteria was the main concern though the TVC was apparently not so high. Besides this, the predominant types of bacteria of the family Enterobacteraceae and vibrionaceae were found (E.g. Plesiomonas, Enterobacter, Escherichia coli, Vibrio spp etc. Salmonella spp were isolated from...
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