Posted by admin on Mar 1, 2014 in |
Now day’s formulation research is breaking barriers of conventional methods. Today, active ingredients can be delivered with a level of convenience, performance and bioavailability never seen in the market place. Fast disintegrating or Mouth dissolving tablet (MDTs) is one such novel approach to increase consumer acceptance by virtue of rapid disintegration, self-administration without water or chewing. The objective of this research was to develop the mouth dissolving tablets platform for sildenafil citrate for pediatric patient in pulmonary hypertension. Mouth dissolving tablets platform was developed using mannitol, crosspovidone, low substituted hydroxypropyl cellulose and povidone. Sucralose and orange flavor are used as sweetener and taste masking agent respectively. Spray dried fluid bed processed technology was utilized to prepare granules. Direct compression technology was used to prepare tablets. Response Surface Methodology was utilized to establish relationships between identified independent variables and selected responses. The quality of mouth dissolving tablets was evaluated by evaluation of the in vitro release profiles, as well as friability, crushing and tensile strength, disintegration time, wetting time, in...
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Posted by admin on Mar 1, 2014 in |
A gradient reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the determination of Prasugrel hydrochloride and its related substances. The well chromatographic separation of prasugrel from its seven related substances and degradation products was achieved on Sunfire C18, 5mm (250mm x 4.6mm) column temperature maintained at 45°C with a mobile phase A: 0.1% v/v orthophosphoric acid in water and mobile phase B: 0.1% v/v orthophosphoric acid in acetonitrile. The flow rate was 1.0mL/min, and the detection wavelength was 220nm. The developed method was validated for specificity, forced degradation studies, sensitivity (LOD and LOQ), linearity, precision (system precision, method precision and intermediate precision), accuracy, stability of standard and sample solutions and robustness. The method is linear with a concentration range of 0.085-3.218µg/ml with correlation coefficients more than 0.9997 for prasugrel and its related substances. The method recoveries obtained are ranged between 96.4% -101.1% for LOQ levels and 94.7%-103.3% for remaining levels. The method was found to be specific, linear, sensitive, precise, rugged, accurate, robust and...
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Posted by admin on Mar 1, 2014 in |
Peperomia pellucida L. (H.B.K.)is an arboreal species occurring in the secondary vegetation in Amazonia. This study aims to perform anatomy analysis by light microscopy and microchemistry analysis of leaves and petiole of P. pellucida. It was observed that the cells, on the adaxial surface, are juxtaposed with irregular shape and heterodimensional and straight walls and, the abaxial surface is formed by juxtaposed and irregularly shaped cells with thin and heterodimensional walls. The microchemical tests showed the presence of secondary metabolites, mainly in the palisade tissue, such as alkaloids, flavonoids and starch. Such anatomical and microchemical features are fundamental in the characterization of the...
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Posted by admin on Mar 1, 2014 in |
The study was carried out to assess the antibacterial & cytotoxic activities of extracts from leaf parts of the plant, Averrhoa bilimbi. Ethanol, methanol and water were used as solvents and antibacterial & cytotoxic effects were measured using disc diffusion test & brine shrimp lethality bioassay (BSLB). The susceptibility of the microorganisms to the extracts of this plant was compared with standard antibiotic cefadroxil. Water extract exhibited more potent antimicrobial activity by inhibiting wide range of gram positive and gram negative bacteria in comparison to ethanolic and methanolic extract of leaves of A. bilimbi. On the other hand, the ethanolic extract of A. bilimbi leaves( EEL) was found to be the most toxic to Brine Shrimp nauplii, with LC50 of 3.7 μg/ml whereas anticancer drug vincristine sulfate (VS) proved LC50 value 1.73 μg/ml indicating that the potent cytotoxic compounds in this plant have affinity for non- polar solvents. The spectrum of activity observed in the present study may provide the indication that the plant could be a possible source...
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Posted by admin on Mar 1, 2014 in |
A simple, rapid, accurate, precise and economical RP- HPLC method with UV detection was optimized, developed and validated as per ICH-Q2 guideline for the simultaneous estimation of Esomeprazole magnesium trihydrate (ESO), Itopride hydrochloride (ITO) and Mosapride citrate (MOSA) in laboratory sample. An optimum condition of separation and detection was developed on a reverse-phase Supelco 516 C18 DB column (250mm×4.6mm i.d., 5µ particle size), using a mobile phase composition of phosphate buffer (20mM, pH-7.4 adjusted with sodium hydroxide):acetonitrile:methanol in the ratio of a 20:20:60 (%v/v) at a flow rate of 1.0 ml/min with UV detection at 275 nm within 6 min with retention time of 3.09, 3.89 and 5.19 for ESO, ITO and MOSA respectively. The standard curves were linear over the concentration range of 2-12 μg/mL, 7.5-45 μg/mL and 1.5-9 μg/mL for ESO, ITO and MOSA respectively with R2 more than 0.999. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification. From the validation results it was concluded that proposed method...
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