Posted by admin on May 1, 2014 in |
Context: Malaria endemic disease remains the most common tropical parasitic disease in the world. According to the World Health Organization (WHO), malaria is the first endemic disease of which humanity is paying a heavy price. Malaria is a major public health problem in Côte d’Ivoire. Objective: To evaluate the in vitro chemosensitivity to Lumefantrine (LUM) and analyze Pfmdr-1gene polymorphism in isolates of Plasmodium falciparum from Abidjan. Materials and Methods: The in vitro activity of Lumefantrine (LUM) was performed using the optical variant of microtest of the World Health Organization (WHO). After extraction, plasmodial falciparum DNA fragments were amplified by PCR method. The study of Pfmdr-1 polymophism was performed after sequencing of amplicons. The analysis of the relationship between the observed mutations and chemosensitivity of isolates was performed using Cohen’s kappa test. Results: Out of 64 Plasmodium falciparum isolates tested, 57 (89%) gave cleare results of in vitro culture. It was found that 67% of them were susceptible to LUM against 33% that were resistant. Molecular study performed on 28...
Read More
Posted by admin on May 1, 2014 in |
Thuja occidentalis L. is a plant widely used therapeutically. Having seen the necessity to standardize the vegetal drug and to evaluate the interference of certain environmental and seasonal factors on the synthesis of metabolites, notably flavonoids, this work considered anatomical and physical-chemical characterization assays for the aforesaid vegetal drug, in addition to phytochemical prospecting and evaluation of the flavonoid content for one year. Characterization demonstrated that a thick powder (484 µm), with loss by desiccation (6.9%), water determination (5.3%), ash content (4.5%) and volatile oils (0.7%) to be acceptable. The average content of total polysaccharides obtained for analysis was 233.2 mg/g. The anatomical evaluation pointed to structures such as spongy parenchyma, secretor channels, heteromorphic cells and ciclocitic stomata. Phytochemical prospecting showed the presence of the majority of the metabolites investigated in all the months of study. Alkaloids and coumarins were absent. The verified results for flavonoid content indicate that T. occidentalis presents a dynamic metabolism with maximum attainment of flavonoids in march/2012, with a suggestive relationship with the verified...
Read More
Posted by admin on May 1, 2014 in |
A new series of thiazolo [3′,2′;1,2] pyrimido [5,4,e] pyrimidines and thiazolo [3′,2′;1,2] [1,2,4] triazolo [3”,4”-c]pyrimido [5,4-e] pyrimidines linked to 2-pyrazolin-5-one moiety were prepared by derivatization of the hydrazine functionality of the heterocyclic hydrazine with various reagents. The newly synthesized compounds were characterized by elemental analyses and spectroscopic techniques. The antimicrobial, anti-inflammatory, and analgesic activities of the synthesized compounds were evaluated using agar diffusion method, carrageenan-induced paw edema, and writhing assays, respectively. The finding of the study showed that, compounds 1a, b, 3a, b, 7b and 15a, b have excellent antimicrobial potency. In the anti-inflammatory activity, compounds 2a and 2b showed remarkable activities (30% and 32%, respectively). For the analgesic activity, the same compounds 2a and 2b gave significant activities (27% and 29%,...
Read More
Posted by admin on May 1, 2014 in |
Rapid expansion of supercritical solution (RESS) has provided a promising alternative to produce ultrafine particles of heat-sensitive materials. Stearic acid is a good lipid for development of solid lipid nanoparticles (SLNs) in drug delivery systems. Therefore, currently, much research on the micronization of stearic acid is going on. In this study, formation of submicron stearic acid was reported by using RESS process. Thanks to high solubility of stearic acid in supercritical CO2, RESS can be used to produce stearic acid nanoparticles and so in this process, extraction temperature and pressure are a bit more than critical point of SC-CO2. The unprocessed and processed stearic acid powders were characterized by means of scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and Fourier transform infrared spectrophotometry (FT-IR). FT-IR analysis and XRD pattern of processed stearic acid showed that the degree of crystallinity was reduced without any chemical structural change. DSC analysis showed a 2.7◦C decrease in the melting point from that of bulk stearic acid. Also, the RESS...
Read More
Posted by admin on May 1, 2014 in |
Citrullus colocynthis is a wild common plant in arid and semi arid areas of Algeria which are characterized by high incidence of scorpion envenomation. It is used in Algeria to treat many diseases. In order to optimize and develop new therapeutic strategies for scorpion envenoming, a phytotherapeutic approach has been investigated. Pharmacological properties of Citrullus colocynthis were evaluated as new approach based on their local and systemic beneficial effects after scorpion envenomation mainly Androctonus australis hector venom (Aah). Local action (paw edema) and systemic effects (inflammatory, metabolic parameters, oxidative stress and hyperglycemia) were studied in pretreated mice with Citrullus colocynthis (50 mg/kg), 30 min before injection of sublethal dose of Aah venom (10 µg/20 g). Obtainedresults showed thatinjected Citrullus colocynthis before envenomation is able to protect animals against the toxicity of the venom. It significantly reduced paw edema, cell migration, exudation, hyperglycemia, and MDA. Citrullus colocynthis decreased also some inflammatory markers (MPO and EPO activities, CRP and C3) and maintain the level of CPK, ASAT and ALAT. Citrullus colocynthis...
Read More
